Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,375,330	A→C	V154V (GTA→GTC)	dcuD →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,375,330	0	A	C	100.0%	47.1 / NA	15	V154V (GTA→GTC)	dcuD	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/4);  total (11/4)

GTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAGCGCCTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTAAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTC  >  NC_000913/3375186‑3375463                                                                                                                                                 |                                                                                                                                      gTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAGCGCCTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAgtcgt                                                                                                                                 >  1:220330/1‑151 (MQ=255)                      gTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGTCGCAGTTTATTACCAGCGCTTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTAt                                                                                                             >  1:102553/1‑150 (MQ=255)                        atatTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAGCGCCTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCg                                                                                                           >  1:82394/1‑150 (MQ=255)                                                                      gTTTATTACCAGCGCCTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCAtt                                                            >  1:79144/1‑151 (MQ=255)                                                                      gTTTATTACCAGCGCCTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCAtt                                                            >  2:151812/1‑151 (MQ=255)                                                                                    ccTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGt                                              >  1:119399/1‑151 (MQ=255)                                                                                     cTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTa                                             <  1:164437/151‑1 (MQ=255)                                                                                       ccGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGc                                           <  1:132038/151‑1 (MQ=255)                                                                                              gggTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATg                                    >  1:313665/1‑151 (MQ=255)                                                                                                  aTGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGaaaa                                >  1:35668/1‑151 (MQ=255)                                                                                                     ttGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTGCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTg                             >  1:72721/1‑151 (MQ=255)                                                                                                                  ccTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATActtctt                >  1:161133/1‑151 (MQ=255)                                                                                                                  ccTTATTTCCGACGCTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATActtctt                >  2:278557/1‑151 (MQ=255)                                                                                                                                cTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTc  <  1:94496/151‑1 (MQ=255)                                                                                                                                cTGGTGAGTCTGGGAGTCAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTc  <  2:104355/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                      GTTAAAACTCATTCGCTCGCCGTATATTATTCTGTCGGCAACTTACGTCATCGGCCAAATCATGGCGCAGTTTATTACCAGCGCCTCCGGTCTGGGTATGTTGCTGATGGTCACCTTATTTCCGACGCTGGTGAGTCTGGGAGTAAGTCGTCTCTCTGCGGTGGCAGTTATCGCAACCACGATGTCCATTGAGTGGGGGATTCTGGAAACGAACTCCATTTTTGCTGCCCAGGTAGCGGGAATGAAAATTGCCACATACTTCTTCCACTACCAGCTTC  >  NC_000913/3375186‑3375463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑