Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	184,675	A→C	L132* (TTA→TGA)	yaeI ←	phosphodiesterase with model substrate bis‑pNPP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	184,675	0	A	C	100.0%	38.6 / NA	12	L132* (TTA→TGA)	yaeI	phosphodiesterase with model substrate bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/6);  total (6/6)

GCATTGTCCGGCCCATAAGTCACCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAA  >  NC_000913/184554‑184820                                                                                                                          |                                                                                                                                                  gCATTGTCCGGCCCATAAGTCACCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGa                                                                                                                      >  1:190179/1‑151 (MQ=255)                                   aGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGca                                                                                     >  2:50252/1‑150 (MQ=255)                                                                               aaaCAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAAtt                                         >  1:261564/1‑150 (MQ=255)                                                                                  caacaCCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAg                                      >  1:188927/1‑150 (MQ=255)                                                                                    acacCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGa                                   >  1:11740/1‑151 (MQ=255)                                                                                               cccGCTGATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAaat                         <  2:298447/150‑1 (MQ=255)                                                                                                ccGCTGATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAaataa                       <  2:218061/151‑1 (MQ=255)                                                                                                    tGATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACg                   <  1:209464/151‑1 (MQ=255)                                                                                                     gATTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGt                  >  2:314890/1‑151 (MQ=255)                                                                                                      aTTTCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGt                  <  1:161612/150‑1 (MQ=255)                                                                                                        ttCAACGTCTCGCCAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAAt               <  1:67322/151‑1 (MQ=255)                                                                                                                     cAATTCAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTaa  <  1:89934/151‑1 (MQ=255)                                                                                                                          |                                                                                                                                                  GCATTGTCCGGCCCATAAGTCACCAGTGCCTACCAGTTCGAATTGCCTGTTCGGCGTGGCGATCACCGTAGCCTGGTTAAACAACACCGTGATGCCCGCTGATTTCAACGTCTCGCCAATTAAGTGATTTTTTTCTGTACCAACAGGGCGATCGTGGTTGCCGAAGCAGGCAAACGTCGGCGCACATTCGGCAAGGGGGGAGAGTACGTCACTAAACGCCGAAAAATTCAGCGACATATCAAATAATACGTAATCGCCGCCCAGTAA  >  NC_000913/184554‑184820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑