Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,792,236	T→G	I106L (ATT→CTT)	kbl ←	glycine C‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,792,236	0	T	G	100.0%	94.6 / NA	27	I106L (ATT→CTT)	kbl	glycine C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/10);  total (17/10)

CTTTGCACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAG  >  NC_000913/3792110‑3792376                                                                                                                               |                                                                                                                                             cTTTGCACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAg                                                                                                                        <  1:179172/149‑1 (MQ=255)          gACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGttt                                                                                                             >  1:210440/1‑151 (MQ=255)          gACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGttt                                                                                                             >  2:302974/1‑151 (MQ=255)          gACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGttt                                                                                                             >  1:249994/1‑151 (MQ=255)                  ccATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCaa                                                                                                     >  1:185562/1‑151 (MQ=255)                  ccATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCaa                                                                                                     >  1:262017/1‑151 (MQ=255)                  ccATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCaa                                                                                                     >  2:128065/1‑151 (MQ=255)                  ccATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCaa                                                                                                     >  1:61673/1‑151 (MQ=255)                  ccATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCaa                                                                                                     >  2:1114/1‑151 (MQ=255)                  ccATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCaa                                                                                                     >  1:83708/1‑151 (MQ=255)                    aTCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAg                                                                                                    >  2:236883/1‑150 (MQ=255)                    aTCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAg                                                                                                    >  2:168913/1‑150 (MQ=255)                      cAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGct                                                                                                  >  1:144919/1‑150 (MQ=255)                         taataGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCttt                                                                                              >  2:95657/1‑151 (MQ=255)                         taataGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCttt                                                                                              >  1:119211/1‑151 (MQ=255)                               aCGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGc                                                                                         <  2:214708/150‑1 (MQ=255)                               aCGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGc                                                                                         <  2:319814/150‑1 (MQ=255)                                  cgTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATgg                                                                                          >  1:29241/1‑146 (MQ=255)                                        tCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGt                                                                               <  2:306831/151‑1 (MQ=255)                                            tgaggcGGAGATAATGGCGTCTTCCGCGCCCAGAAGCGTTTCAAACAGGCCCCCGTTATCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCTGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGc                                                                             <  2:90335/144‑1 (MQ=255)                                                          tGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCAc                                                             <  1:53526/151‑1 (MQ=255)                                                          tGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCAc                                                             <  1:41742/151‑1 (MQ=255)                                                                 ttCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGcc                                                      <  2:72419/151‑1 (MQ=255)                                                                                               aCCGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGcc                        <  1:246848/151‑1 (MQ=255)                                                                                                ccGTTAGCATCAAAGCAGGAAGAGTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGc                         >  2:41696/1‑149 (MQ=255)                                                                                                               cAGGAAGAGTAGAGAAGCGCATCTTCCACCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc         <  2:25217/150‑1 (MQ=255)                                                                                                                     gagTAGAGAAGCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcagatcag  >  1:66402/1‑151 (MQ=255)                                                                                                                               |                                                                                                                                             CTTTGCACAGACGCACACCATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAG  >  NC_000913/3792110‑3792376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑