Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,835,453	G→A	G159G (GGC→GGT)	yicJ ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,835,453	0	G	A	100.0%	128.8 / NA	37	G159G (GGC→GGT)	yicJ	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/25);  total (12/25)

CAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCAT  >  NC_000913/3835305‑3835598                                                                                                                                                     |                                                                                                                                                  cAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATAcc                                                                                                                                                 <  1:137098/151‑1 (MQ=255)   aCGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCt                                                                                                                                                >  1:218317/1‑150 (MQ=255)          cTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGt                                                                                                                                        >  2:243159/1‑151 (MQ=255)                        aaaCAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAAc                                                                                                                          <  2:175340/151‑1 (MQ=255)                               aTGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGa                                                                                                                   >  1:221616/1‑151 (MQ=255)                                          atGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCgaga                                                                                                        <  1:77621/151‑1 (MQ=255)                                           tGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGAt                                                                                                       <  2:71906/151‑1 (MQ=255)                                                    ccacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGt                                                                                              >  1:118275/1‑151 (MQ=255)                                                    ccacGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGt                                                                                              >  2:290638/1‑151 (MQ=255)                                                        ggAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCggg                                                                                          >  1:86759/1‑151 (MQ=255)                                                                   cgATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGAt                                                                               >  1:7799/1‑151 (MQ=255)                                                                     aTACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGAtt                                                                              <  1:44370/150‑1 (MQ=255)                                                                        ccGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTacac                                                                          <  1:174671/151‑1 (MQ=255)                                                                           cccTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTAcaccac                                                                       <  2:118160/151‑1 (MQ=255)                                                                             cTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCAccc                                                                     <  1:16227/151‑1 (MQ=255)                                                                                  aaCCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATgc                                                                <  1:166824/151‑1 (MQ=255)                                                                                          ggTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAg                                                        >  2:86660/1‑151 (MQ=255)                                                                                            tttATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAggg                                                      <  2:272242/151‑1 (MQ=255)                                                                                              tattatCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGat                                                    <  1:270485/151‑1 (MQ=255)                                                                                              tattatCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGat                                                    <  1:129479/151‑1 (MQ=255)                                                                                                  atCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTg                                                <  1:218812/151‑1 (MQ=255)                                                                                                  atCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTg                                                <  2:276168/151‑1 (MQ=255)                                                                                                   tCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTga                                               <  1:102266/151‑1 (MQ=255)                                                                                                          cAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGt                                        >  2:269111/1‑151 (MQ=255)                                                                                                                aattaaCCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAg                                  <  1:263135/151‑1 (MQ=255)                                                                                                                 attaaCCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGGGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAgt                                 <  1:105793/151‑1 (MQ=255)                                                                                                                 attaaCCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAgt                                 <  2:305132/151‑1 (MQ=255)                                                                                                                 attaaCCAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAgt                                 <  2:95825/151‑1 (MQ=255)                                                                                                                      ccAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAgtaaggt                            <  1:175207/151‑1 (MQ=255)                                                                                                                      ccAGTGGCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAgtaaggt                            <  1:114228/151‑1 (MQ=255)                                                                                                                           ggCATCATCAGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTACAAAGTAAGGTAAGTa                       >  2:112274/1‑151 (MQ=255)                                                                                                                                 atcaGAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGgtgt                 <  2:212313/151‑1 (MQ=255)                                                                                                                                     gAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTgtaagt             >  1:294497/1‑151 (MQ=255)                                                                                                                                     gAACAGTAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTgtaagt             >  1:101546/1‑151 (MQ=255)                                                                                                                                           tAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAAt          <  2:112184/148‑1 (MQ=255)                                                                                                                                           tAGAAAGCATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATtgc       <  1:159935/151‑1 (MQ=255)                                                                                                                                                  cATACCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCAt  <  2:211926/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CAACGCGTTCTTTAGTGGTGAAGAAACAAAATGCCAGCATCATGAATGCCACCACGGAAAGGACCGCGATACCGCCCTGGAAACCGAGTGGTTTATTATCACCGCCAATTAAATTAACCAGTGGCATCATCAGAACAGTAGAAAGCATGCCTCCCGCGGTCGCCAGCACAAAACGCCAGGATTGCAGCGAGATACGCTGAGTCGGGTCATTGGTGATTACACCACCCAATGCGCAGTAAGGGATATTGACGACGGTATAAAGTAAGGTAAGTAGGGTGTAAGTAATTGCTGCAT  >  NC_000913/3835305‑3835598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑