Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,874,610	T→G	D184A (GAT→GCT)	dgoR ←	D‑galactonate catabolism operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,874,610	0	T	G	100.0%	106.6 / NA	31	D184A (GAT→GCT)	dgoR	D‑galactonate catabolism operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/15);  total (16/15)

GTCATGTGATTTCCTTTAACCTTCGTGTCGAGCTGGCGATCATGGTAAGCGCCGCCTGCTCTGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCATCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAATATCCGCTTCGTTAAACGCTTCGCGGTCCTGATT  >  NC_000913/3874470‑3874755                                                                                                                                             |                                                                                                                                                  gTCATGTGATTTCCTTTAACCTTCGTGTCGAGCTGGCGATCATGGTAAGCGCCGCCTGCTCTGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCa                                                                                                                                         >  1:147949/1‑151 (MQ=255) gTCATGTGATTTCCTTTAACCTTCGTGTCGAGCTGGCGATCATGGTAAGCGCCGCCTGCTCTGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCa                                                                                                                                         >  2:212124/1‑151 (MQ=255) gTCATGTGATTTCCTTTAACCTTCGTGTCGAGCTGGCGATCATGGTAAGCGCCGCCTGCTCTGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCa                                                                                                                                         >  1:92665/1‑151 (MQ=255)     tgtgATTACCTTTACCCTTCGTGTCGAGCTGGCGATCATGGTAAGCGCGGACTGCTATGCCGCATCGACGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGGTTGCGGCATATTGGCATCAGGTCCCATCCAggat                                                                                                                                     <  2:162451/151‑3 (MQ=255)        gATTTCCTTTAACCTTCGTGTCGAGCTGGCGATCATGGTAAGCGCCGCCTGCTCTGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGgttcgt                                                                                                                                  >  2:272205/1‑151 (MQ=255)                                                   gccgccTGCTCTGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGtt                                                                                       >  1:305650/1‑151 (MQ=255)                                                    ccgccTGCTCTGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTg                                                                                      >  2:318235/1‑151 (MQ=255)                                                             ctGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAacac                                                                             <  2:92665/151‑1 (MQ=255)                                                             ctGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAacac                                                                             <  1:191135/151‑1 (MQ=255)                                                             ctGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAacac                                                                             <  1:286120/151‑1 (MQ=255)                                                             ctGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAacac                                                                             <  1:71600/151‑1 (MQ=255)                                                               gCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCg                                                                           >  2:296237/1‑151 (MQ=255)                                                                   cATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGt                                                                        <  2:293057/150‑1 (MQ=255)                                                                         cGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCAc                                                                 <  1:122765/151‑1 (MQ=255)                                                                         cGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCAc                                                                 <  1:297900/151‑1 (MQ=255)                                                                          gTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCAc                                                                 >  1:12536/1‑150 (MQ=255)                                                                                aTGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTg                                                           >  2:44820/1‑150 (MQ=255)                                                                                     gTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTgcagca                                                      >  2:158174/1‑150 (MQ=255)                                                                                      tATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCAc                                                     <  2:57003/150‑1 (MQ=255)                                                                                      tATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCAc                                                     <  2:228146/150‑1 (MQ=255)                                                                                      tATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCAc                                                     <  2:233852/150‑1 (MQ=255)                                                                                      tATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCAc                                                     <  2:242741/150‑1 (MQ=255)                                                                                          gCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGc                                                  >  2:201867/1‑149 (MQ=255)                                                                                          gCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGc                                                  >  1:126882/1‑149 (MQ=255)                                                                                                       ccTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAat                                   >  2:26300/1‑151 (MQ=255)                                                                                                         ttATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAatat                                 >  1:248851/1‑151 (MQ=255)                                                                                                          tATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAatat                                 >  2:64060/1‑150 (MQ=255)                                                                                                                       cGTTTGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAATATCCGCTTCGTTAAAc                   <  1:205040/151‑1 (MQ=255)                                                                                                                         tttGCGGCATGTTGGCCTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAATATCCGCTTCGTTAAACGc                 <  2:109529/151‑1 (MQ=255)                                                                                                                                 aTGTTGGCCTCAGCGCCCACCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAATATCCGCTTCGTTAAACGCTTCGCGGt         >  2:125394/1‑151 (MQ=255)                                                                                                                                        ccTCAGCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAATATCCGCTTCGTTAAACGCTTCGCGGTCCTGAtt  <  1:173956/151‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  GTCATGTGATTTCCTTTAACCTTCGTGTCGAGCTGGCGATCATGGTAAGCGCCGCCTGCTCTGCCGCATCGCCGTCCTGATGTCGTATCGCATCGAACAGCGCCTTATGTTCCTGGAGCGTTTGCGGCATGTTGGCCTCATCGCCCATCCAGGTTCGTTCAAAAACCGCCCGCTGCAGCGAACTGATCGCAATGCTAAGTTGCTGTAACACCGGGTTATGCACCGACTGCAGCACCGCCTCGTGGTAGCGAATATCCGCTTCGTTAAACGCTTCGCGGTCCTGATT  >  NC_000913/3874470‑3874755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑