Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,988,430	C→T	V213I (GTA→ATA)	hemX ←	putative uroporphyrinogen III methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,988,430	0	C	T	100.0%	61.7 / NA	18	V213I (GTA→ATA)	hemX	putative uroporphyrinogen III methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/8);  total (10/8)

GTAATGAAGTTGTCCATAAAGTTCTGCCAGCTTTTTTGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGC  >  NC_000913/3988291‑3988564                                                                                                                                            |                                                                                                                                       gTAATGAAGTTGTCCATAAAGTTCTGCCAGCTTTTTTGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGaaa                                                                                                                             >  1:245017/1‑151 (MQ=255)  tAATGAAGTTGTCCATAAAGTTCTGCCAGCTTTTTTGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGaaa                                                                                                                             <  1:214858/150‑1 (MQ=255)  tAATGAAGTTGTCCATAAAGTTCTGCCAGCTTTTTTGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGaaa                                                                                                                             <  1:193424/150‑1 (MQ=255)                                   tttGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATc                                                                                           <  1:57331/151‑1 (MQ=255)                                           ttGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCg                                                                                   >  2:170598/1‑151 (MQ=255)                                           ttGATACGACATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCg                                                                                   >  2:91780/1‑151 (MQ=255)                                                  gCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTg                                                                             >  2:51720/1‑150 (MQ=255)                                                   ccATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCa                                                                           >  1:234965/1‑151 (MQ=255)                                                    cATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAg                                                                          <  2:294392/151‑1 (MQ=255)                                                                gAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGa                                                              >  1:185356/1‑151 (MQ=255)                                                                gAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGa                                                              >  1:94723/1‑151 (MQ=255)                                                                                  ccGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGcc                                             >  1:156680/1‑150 (MQ=255)                                                                                  ccGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGACTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGcc                                             >  1:113590/1‑150 (MQ=255)                                                                                     tCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCcgac                                         <  1:55132/151‑1 (MQ=255)                                                                                        gAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGaa                                      >  1:157344/1‑151 (MQ=255)                                                                                                                tCATTATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGtcattc               <  1:236967/150‑1 (MQ=255)                                                                                                                    tATCGGCCAGACGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATc          <  1:117915/151‑1 (MQ=255)                                                                                                                              aCGCAGGTTATCTATCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGc  <  2:318591/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       GTAATGAAGTTGTCCATAAAGTTCTGCCAGCTTTTTTGCAGATTGATACGCCATTCGCTGATGGAACTGGAAAGCTCTTCACCGTCTGAATCCATCGGCGAACCATCGCTGTCATTATCGGCCAGACGCAGGTTATCTACCTGATTTGAAAGCTGATTAAGCTTAAGGATGATGCCGTCATAATCCACCTGCGATACTGCAGAAAGGCTGGCGATATCATCGGTAATTGCCCGACGAACGGTAATCAGACTCGGGTCATTCATATCCGCCAGGC  >  NC_000913/3988291‑3988564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑