Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,318	T→G	H258P (CAT→CCT)	glpF ←	glycerol facilitator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,318	0	T	G	100.0%	66.5 / NA	20	H258P (CAT→CCT)	glpF	glycerol facilitator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/13);  total (7/13)

CGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCG  >  NC_000913/4117170‑4117462                                                                                                                                                     |                                                                                                                                                 cgGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAggg                                                                                                                                                <  1:85752/151‑1 (MQ=255)     aGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGa                                                                                                                                             >  2:167942/1‑150 (MQ=255)             gtgCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAg                                                                                                                                     <  2:245687/150‑1 (MQ=255)             gtgCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAg                                                                                                                                     <  2:145276/150‑1 (MQ=255)                         aGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGc                                                                                                                         <  1:15263/150‑1 (MQ=255)                                       ttttttttCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAAt                                                                                                          <  2:20035/151‑1 (MQ=255)                                         ttttttCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATcg                                                                                                        <  1:20092/151‑1 (MQ=255)                                                   cATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGAt                                                                                              <  2:313771/151‑1 (MQ=255)                                                              gTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGaaaa                                                                                   >  1:67274/1‑151 (MQ=255)                                                                      gTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCAc                                                                            >  1:148717/1‑150 (MQ=255)                                                                        cATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAg                                                                         >  1:292159/1‑151 (MQ=255)                                                                         atatTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAgg                                                                        >  1:186142/1‑151 (MQ=255)                                                                            ttACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAg                                                                     >  1:308770/1‑151 (MQ=255)                                                                                  cGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGa                                                               <  2:120782/151‑1 (MQ=255)                                                                                    aaGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGaa                                                              <  2:47416/150‑1 (MQ=255)                                                                                       cTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGGTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGtc                                                          <  2:196358/151‑1 (MQ=255)                                                                                         ttttGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGtctc                                                        <  1:133905/151‑1 (MQ=255)                                                                                                          tgtgGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCg                                       <  1:245144/151‑1 (MQ=255)                                                                                                               tttCCTTTTCTTCCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACAt                                   <  1:16530/150‑1 (MQ=255)                                                                                                                        cttcCACAACACAGATATCGCAAGGCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGcc                         <  2:309929/151‑1 (MQ=255)                                                                                                                                               ggCAAAGGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCg  >  1:211289/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGACCAATCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCG  >  NC_000913/4117170‑4117462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑