Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,236,154 T→G intergenic (+6/‑108) yjbE → / → yjbF extracellular polysaccharide production threonine‑rich protein/extracellular polysaccharide production lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,236,1540TG100.0% 78.7 / NA 22intergenic (+6/‑108)yjbE/yjbFextracellular polysaccharide production threonine‑rich protein/extracellular polysaccharide production lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/5);  total (17/5)

AACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATT  >  NC_000913/4236007‑4236299
                                                                                                                                                   |                                                                                                                                                 
aaCGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAggatg                                                                                                                                                <  2:239711/151‑1 (MQ=255)
                                   gCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTgg                                                                                                             >  2:277262/1‑151 (MQ=255)
                                   gCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTgg                                                                                                             >  2:156854/1‑151 (MQ=255)
                                    cGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTgg                                                                                                             >  1:248704/1‑150 (MQ=255)
                                            ccGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTa                                                                                                    >  2:4955/1‑151 (MQ=255)
                                                              gtgtCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATtaaccataa                                                                                  >  1:258320/1‑151 (MQ=255)
                                                               tgtCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATtaaccataac                                                                                 <  1:76645/151‑1 (MQ=255)
                                                                    cgcTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCacac                                                                            <  2:319780/151‑1 (MQ=255)
                                                                                       cGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTg                                                          >  1:110518/1‑150 (MQ=255)
                                                                                               gTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTgg                                                 >  1:47836/1‑151 (MQ=255)
                                                                                                ttCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGga                                                >  2:77097/1‑151 (MQ=255)
                                                                                                      tACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAagag                                          >  2:162686/1‑151 (MQ=255)
                                                                                                      tACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAagag                                          <  2:237566/151‑1 (MQ=255)
                                                                                                      tACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAagag                                          >  2:250102/1‑151 (MQ=255)
                                                                                                               caccacAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAg                                  >  2:258715/1‑150 (MQ=255)
                                                                                                                 ccacAACCACGACCACCAGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGa                               >  1:145337/1‑151 (MQ=255)
                                                                                                                              caccaGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCAtt                   >  1:314965/1‑150 (MQ=255)
                                                                                                                              caccaGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCAtt                   >  1:192910/1‑150 (MQ=255)
                                                                                                                               accaGTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCt                 <  1:147731/151‑1 (MQ=255)
                                                                                                                                   gTACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTAt              >  2:1328/1‑150 (MQ=255)
                                                                                                                                    tACCCAGTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATc             <  1:181842/150‑1 (MQ=255)
                                                                                                                                          gTAATAAAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGc          >  1:264594/1‑147 (MQ=255)
                                                                                                                                              taaAGGATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGttatt  >  2:150293/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCGCTTGCGGCAACCGGTGGCGGTGATGGTTCTAATACCGGGACCACCACAACCACGACCACCAGTACCCAGTAATAAAGTATGTATCCCCAAAATAATTCGAGTCATTGCATCTGTGGCTAGAAGTATGAAGGGATTAACCATAACCACACTCCGGTGTGGTTATTCTGCCCCTCTGGAGAAGAGTCGTGAAGCGACCTGCACTCATTCTTATCTGCCTGTTATT  >  NC_000913/4236007‑4236299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: