Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,388,912	A→C	N153K (AAT→AAG)	mscM ←	mechanosensitive channel protein, miniconductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,388,912	0	A	C	100.0%	42.4 / NA	13	N153K (AAT→AAG)	mscM	mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (10/3);  total (10/3)

TGGCGGTTATTGGCAGACAGCTGCGCCAGCTCCAGTTCATCAACGAGCGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAG  >  NC_000913/4388769‑4389040                                                                                                                                                |                                                                                                                                 tGGCGGTTATTGGCAGACAGCTGCGCCAGCTCCAGTTCATCAACGAGCGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACt                                                                                                                           <  2:180982/151‑1 (MQ=255)        tATTGGCAGACAGCTGCGCCAGCTCCAGTTCATCAACGAGCGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGAcggcg                                                                                                                    >  2:225860/1‑151 (MQ=255)                        cgcCAGCTCCAGTTCATCAACGAGCGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTg                                                                                                        >  1:53004/1‑147 (MQ=255)                                                 gcCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCgg                                                                           >  2:116451/1‑151 (MQ=255)                                                  ccTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCgg                                                                           >  1:8336/1‑150 (MQ=255)                                                           gTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTcgcg                                                                 >  2:16286/1‑151 (MQ=255)                                                           gTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTcgcg                                                                 >  2:76981/1‑151 (MQ=255)                                                                gagTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGgcg                                                             >  1:197453/1‑150 (MQ=255)                                                                               gcgAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCCGCCCTTg                                               >  2:160475/1‑149 (MQ=255)                                                                                    aTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGcc                                        <  1:142467/151‑1 (MQ=255)                                                                                    aTTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGcc                                        <  1:142581/151‑1 (MQ=255)                                                                                                        gAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCaa                    >  2:198906/1‑151 (MQ=255)                                                                                                                          ttCCCAGGCGGCGCTCGATCTCCTTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAAACTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGgag  >  2:201038/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                 TGGCGGTTATTGGCAGACAGCTGCGCCAGCTCCAGTTCATCAACGAGCGCCTTAAGACGTGCAGAGTCAGACTGCAACGCGAAATTTTGTGCCTGATTGAGCGGAGTATTGCCGGTAAGCGTTCCCAGGCGGCGCTCGATCTCATTTAACTGACGGCGGGCGTCGGTTTGCTGTTGCGGCAGTTGATTCAGCGAATCGGCAATCTCGCGGGCGCGCTCCTGCTCTTGCTGGGCCTGACGGCTTTTATCCAGCAACTGGCTGCTGACCTGGAG  >  NC_000913/4388769‑4389040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑