Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,491,346	T→G	N394H (AAC→CAC)	idnT ←	L‑idonate and D‑gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,491,346	0	T	G	100.0%	73.1 / NA	23	N394H (AAC→CAC)	idnT	L‑idonate and D‑gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/11);  total (12/11)

TTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATGGCCGCGACGGTGGCAGAGCCCAGAGCGATACGCAACAG  >  NC_000913/4491198‑4491490                                                                                                                                                     |                                                                                                                                                 ttATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTa                                                                                                                                                <  2:139033/151‑1 (MQ=255)  tATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTAc                                                                                                                                               <  2:99150/151‑1 (MQ=255)     tCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACgtg                                                                                                                                            <  2:23730/151‑1 (MQ=255)       tctCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAAAACATAAGAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTACACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGCCGTGCACgtgag                                                                                                                                          >  2:135280/3‑149 (MQ=255)            gTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGAcgcg                                                                                                                                     >  1:42895/1‑151 (MQ=255)                                  aaCACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTg                                                                                                               <  2:279377/151‑1 (MQ=255)                                         ccAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTAc                                                                                                        <  2:274714/151‑1 (MQ=255)                                                aCCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTaaa                                                                                                 >  1:73661/1‑151 (MQ=255)                                                 cccATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAg                                                                                                >  2:310159/1‑151 (MQ=255)                                                           aaaTAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCgg                                                                                       >  2:172278/1‑150 (MQ=255)                                                                   gTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTaa                                                                              >  1:16643/1‑151 (MQ=255)                                                                     ttCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACg                                                                            >  2:139484/1‑151 (MQ=255)                                                                               gTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCg                                                                  >  1:124169/1‑151 (MQ=255)                                                                                            aCGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAacaccacacc                                                     <  2:241732/151‑1 (MQ=255)                                                                                                 tCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCgg                                                <  1:313638/151‑1 (MQ=255)                                                                                                         cGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATg                                        <  2:284198/151‑1 (MQ=255)                                                                                                         cGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATg                                        <  1:149098/151‑1 (MQ=255)                                                                                                                ttAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATGGCCGCGa                                 >  1:15385/1‑151 (MQ=255)                                                                                                                                   gCCAGAAGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATGGCCGCGACGGTGGCAGAGCCCagag               >  2:25653/1‑150 (MQ=255)                                                                                                                                        aaGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATGGCCGCGACGGTGGCAGAGCCCAGAGCGATAc         <  2:112642/151‑1 (MQ=255)                                                                                                                                         aGCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATGGCCGCGACGGTGGCAGAGCCCAGAGCGATACg        >  2:191103/1‑151 (MQ=255)                                                                                                                                          gCCAGGGTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATGGCCGCGACGGTGGCAGAGCCCAGAGCGATACg        >  2:43563/1‑150 (MQ=255)                                                                                                                                                gTCGTGTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATGGCCGCGACGGTGGCAGAGCCCAGAGCGATACGCAACAg  <  1:217194/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 TTATTCTTTCAGTGCAATACGGCGTTAATGGCTAACACGCCCAGCAAACCCATAATAGAAATAAGGGTTTCCATCACCGTCCAGGTACGCAACGTTTCACCAACCGTCAGATTAAAATACCCTTTAAATAGCCAGAAGCCAGGGTCGTTTACGTGTGACGCGATCACGCTGCCCGCACCGGTTGCCAGTACCATTAAAGCGGGATCGGCATGGGTAACGTTGATAATCGGCAACACCACACCCGCGGTGGTAATGGCCGCGACGGTGGCAGAGCCCAGAGCGATACGCAACAG  >  NC_000913/4491198‑4491490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑