Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,599,861 (T)8→7 intergenic (‑101/‑45) dapA ← / → gcvR dihydrodipicolinate synthase/transcriptional repressor, regulatory protein accessory to GcvA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,599,8540T.100.0% 252.8 / NA 54intergenic (‑94/‑52)dapA/gcvRdihydrodipicolinate synthase/transcriptional repressor, regulatory protein accessory to GcvA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (35/19);  total (35/19)

GACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGT  >  NC_000913/2599704‑2599998
                                                                                                                                                      |                                                                                                                                                
gacaTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGt                                                                                                                                                  >  2:694281/1‑151 (MQ=255)
  caTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGttt                                                                                                                                                >  2:546661/1‑151 (MQ=255)
          ttttCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGtttttttatgc                                                                                                                                        <  2:119965/151‑5 (MQ=255)
          ttttCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGtttttttatgc                                                                                                                                        <  1:572841/151‑5 (MQ=255)
                    tcGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAg                                                                                                                             >  2:81137/1‑151 (MQ=255)
                       gAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGaa                                                                                                                           >  1:11732/1‑150 (MQ=255)
                       gAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGaa                                                                                                                           <  1:245008/150‑1 (MQ=255)
                       gAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGaa                                                                                                                           >  2:151520/1‑150 (MQ=255)
                       gAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGaa                                                                                                                           >  2:736465/1‑150 (MQ=255)
                             cAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCa                                                                                                                    >  2:499159/1‑151 (MQ=255)
                                 cgcgACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCAcccc                                                                                                                <  1:226935/151‑1 (MQ=255)
                                        aTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAgg                                                                                                         <  2:279642/151‑1 (MQ=255)
                                                   gAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGtt                                                                                              >  2:37752/1‑151 (MQ=255)
                                                   gAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGtt                                                                                              >  2:405824/1‑151 (MQ=255)
                                                   gAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGtt                                                                                              >  2:429891/1‑151 (MQ=255)
                                                    aaCATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGttt                                                                                             >  2:153058/1‑151 (MQ=255)
                                                       aTGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGa                                                                                           >  1:611303/1‑150 (MQ=255)
                                                        tGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGaca                                                                                         >  2:789557/1‑151 (MQ=255)
                                                         gggCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGaca                                                                                         >  2:312605/1‑150 (MQ=255)
                                                          ggCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGacac                                                                                        <  1:165106/150‑1 (MQ=255)
                                                           gCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGaca                                                                                         >  2:773197/1‑148 (MQ=255)
                                                                ccTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCAt                                                                                  >  2:569334/1‑150 (MQ=255)
                                                                   ctGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCa                                                                              <  2:553006/151‑1 (MQ=255)
                                                                     gtgCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCaa                                                                             >  1:322983/1‑150 (MQ=255)
                                                                     gtgCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCaa                                                                             >  1:20144/1‑150 (MQ=255)
                                                                              aGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTgg                                                                   >  2:779381/1‑151 (MQ=255)
                                                                                tgtCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTgg                                                                   <  2:86460/149‑1 (MQ=255)
                                                                                tgtCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTgg                                                                   <  2:326335/149‑1 (MQ=255)
                                                                                  tctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGa                                                                <  1:151520/150‑1 (MQ=255)
                                                                                  tctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGAt                                                               >  2:718487/1‑151 (MQ=255)
                                                                                  tctcAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGAt                                                               >  2:259703/1‑151 (MQ=255)
                                                                                              gTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTggg                                                   >  2:661824/1‑151 (MQ=255)
                                                                                              gTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTggg                                                   >  2:615820/1‑151 (MQ=255)
                                                                                              gTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTggg                                                   >  2:532989/1‑151 (MQ=255)
                                                                                                   gTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCg                                              >  1:89059/1‑151 (MQ=255)
                                                                                                      tGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTAAGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATc                                           <  1:643360/151‑1 (MQ=255)
                                                                                                         cATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGc                                         >  1:835280/1‑150 (MQ=255)
                                                                                                          aTTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGccc                                       <  2:523764/151‑1 (MQ=255)
                                                                                                              aaaGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGa                                   >  2:572245/1‑151 (MQ=255)
                                                                                                              aaaGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGa                                   >  2:548767/1‑151 (MQ=255)
                                                                                                               aagcaagcAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGaa                                  <  2:718783/151‑1 (MQ=255)
                                                                                                                  catgcaGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTTCGTAAGAACAACTAACCCCTTAAAGGAACAACCAGTTTGACACTGTCATCGCAACATTATCTGGGGATCACTGCGTTGGGTGCCGATCGCCCTGGatg                                 <  1:182337/146‑3 (MQ=255)
                                                                                                                  caagcaGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAAt                                 <  2:453854/149‑1 (MQ=255)
                                                                                                                  caagcaGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAAt                                 <  1:504690/149‑1 (MQ=255)
                                                                                                                      cagacagaACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGa                            <  2:730443/150‑1 (MQ=255)
                                                                                                                            gaACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCAt                     >  2:578583/1‑151 (MQ=255)
                                                                                                                            gaACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCAt                     >  2:580437/1‑151 (MQ=255)
                                                                                                                            gaACCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCAt                     >  2:439829/1‑151 (MQ=255)
                                                                                                                             aaCCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAacac                        <  1:677969/147‑1 (MQ=255)
                                                                                                                             aaCCGTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAacac                        <  2:767198/147‑1 (MQ=255)
                                                                                                                                 gTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCAcc                 >  2:301059/1‑150 (MQ=255)
                                                                                                                                 gTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCAcc                 >  1:276664/1‑150 (MQ=255)
                                                                                                                                 gTTCTGATTGTTGTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCAcc                 >  1:452684/1‑150 (MQ=255)
                                                                                                                                        ttgttgTATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGt         >  2:452370/1‑151 (MQ=255)
                                                                                                                                             gtATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTCAAGGAATAACCAGTTTTACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagta    >  2:394885/1‑151 (MQ=255)
                                                                                                                                             gtATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGt                                                       >  1:361028/1‑100 (MQ=255)
                                                                                                                                             gtATGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGt                                                       <  2:361030/100‑1 (MQ=255)
                                                                                                                                               aTGCATGTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagtagt  <  2:128708/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
GACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGT  >  NC_000913/2599704‑2599998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: