Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,237	0	C	T	46.4%	96.0 / 25.4	84	S64L (TCG→TTG)	lsrD	autoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base C (11/34);  new base T (39/0);  total (50/34)
Fisher's exact test for biased strand distribution p-value = 2.79e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTAA  >  NC_000913/1604088‑1604387                                                                                                                                                      |                                                                                                                                                       gCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTc                                                                                                                                                        <  1:954988/150‑1 (MQ=255) gCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCg                                                                                                                                                       <  1:616705/151‑1 (MQ=255)    cGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGttt                                                                                                                                                    >  1:1359809/1‑151 (MQ=255)    cGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGttt                                                                                                                                                    <  2:859388/151‑1 (MQ=255)    cGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGtt                                                                                                                                                     <  2:916594/150‑1 (MQ=255)    cGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGt                                                                                                                                                      <  2:1330254/149‑1 (MQ=255)     gTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTg                                                                                                                                                   <  2:801180/151‑1 (MQ=255)       tATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTgg                                                                                                                                                  <  1:1225502/150‑1 (MQ=255)         ttGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTGGTTc                                                                                                                                               >  1:1230297/1‑151 (MQ=255)            agaTTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTGTTTTGTTTGAc                                                                                                                                            >  2:370089/1‑151 (MQ=255)                   cGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTCGACCATTGGc                                                                                                                                     >  1:319025/1‑151 (MQ=255)                             gCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTGGACCATCGGCCCCTGCGccc                                                                                                                           >  2:909559/1‑150 (MQ=255)                              cAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTTGTTTGTTTGGCCATCGGGCTCTGGGccct                                                                                                                          >  2:762709/1‑149 (MQ=255)                              cAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCcat                                                                                                                          <  1:1179169/151‑1 (MQ=255)                              cAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCcat                                                                                                                          <  1:1367895/151‑1 (MQ=255)                                 ttAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCcattgc                                                                                                                       >  1:1323058/1‑151 (MQ=255)                                   aaCCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTTTTTTGTTTGTCCAACTGCCCCTGCGCcctttcat                                                                                                                     >  2:915943/1‑151 (MQ=255)                                    aCCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATTGGCCTCTGCGCcattgcatt                                                                                                                    <  1:350132/151‑1 (MQ=255)                                     cccGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCcattgcattg                                                                                                                   <  2:265141/151‑1 (MQ=255)                                        gcgAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGc                                                                                                                <  1:1086542/151‑1 (MQ=255)                                         cgAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCg                                                                                                               <  2:1172679/151‑1 (MQ=255)                                           aaTGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGTTTGGTATTTTGTTTTTTTTAACCATCTGCCGCTTCGCCCGTTAATTGGGCggg                                                                                                             >  1:435673/1‑149 (MQ=255)                                             tGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTTGGCCATCGGCCTCTTCGCCATTGCATTGGGGGTACt                                                                                                           >  1:162722/1‑151 (MQ=255)                                              gTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACt                                                                                                           <  2:1192130/150‑1 (MQ=255)                                              gTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTg                                                                                                          <  2:1007149/151‑1 (MQ=255)                                                 aGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGGGGACTGttt                                                                                                       >  1:931141/1‑151 (MQ=255)                                                 aGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGttt                                                                                                       <  2:994784/151‑1 (MQ=255)                                                  gATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTTGGCCATCGGCCCCTGCGCCCTTGCCTTTGGCGGACTGtttt                                                                                                      >  2:265792/1‑150 (MQ=255)                                                   aTCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGGCGCTATTTTGTTTTGTTCGACCATCCGCCCCTGCGCCCCTGCATTGGGCGGACTGttttt                                                                                                     >  2:735089/1‑149 (MQ=255)                                                      tcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTTTTTTGTTTGACCAACGGCCCCTGCGCCCTTGCATTGGGGGTGCTGtttttaa                                                                                                   >  2:1195138/1‑146 (MQ=255)                                                          tatGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTTGTTTGGTTCGACCATCGGCCTCTGCGCCCTTGCATTGGGGGTACTGTTTTAAAggggg                                                                                              >  1:148109/1‑150 (MQ=255)                                                           atGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTCGGCCATCCGCCTCTGCGCCCTTGTCTTTGGGGTACTGTTTTAAAgcggtg                                                                                             >  2:275686/1‑151 (MQ=255)                                                           atGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAgtggt                                                                                              <  1:68053/150‑1 (MQ=255)                                                              ttGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGGCGGTATTTCTTTTGGTTTGACCCTTGGCCTCTTCGCCCTTGCATTTGGCGTACTGTTTCAAAGAGGTGTGc                                                                                          >  1:1112365/1‑151 (MQ=255)                                                              ttGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGGCCCCTGCCCCATTGCATTGGGCGTAATGTTTCAAAGAGGtgtgg                                                                                          >  1:1300830/1‑150 (MQ=255)                                                              ttGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCAACCGCCTCTGCGCCCTTGCATTGGGCGTGCTGTTTTTAAGTGGtgtgg                                                                                          >  2:199887/1‑150 (MQ=255)                                                               tGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTTAAAGTGGGGTGcc                                                                                         >  1:988351/1‑151 (MQ=255)                                                                  tGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTGATATTTTTTTTTGTTCGACCATCGGCCCCTGCGCCCTTGCATTGGGCGTACTGTTTTCAAGTGGGGTGCCggt                                                                                      >  2:1188005/1‑149 (MQ=255)                                                                     tCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGGCCTCTGCGCCCTTGCATTGGGCGTACTGTTTTAAAGTGGGGTGCCGATGcc                                                                                   >  1:173354/1‑151 (MQ=255)                                                                       aGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGc                                                                                 <  2:1124648/151‑1 (MQ=255)                                                                         cACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATTTTTTGTTTTGTTCGACCCTCGGCCCCTGCGCCATTGCATTGGGCGTGCTGTTTCAAAGTGGTGTGccgctgccgctg                                                                               >  2:10120/1‑151 (MQ=255)                                                                         cACCAGTGAC‑TTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTTGACCATCGGCCTCTGCGCCCTTGCATTGGGCGGACTGTTTTCAAGGGGTGTGGCGATGCCGCCgg                                                                              >  1:1248062/1‑151 (MQ=255)                                                                           ccAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCCTCCGCCTCTGCGCCATTGCATTGGGCGGACTGTTTCAAAGTGGGGTGCCGATGCCCCTggg                                                                             >  1:305868/1‑150 (MQ=255)                                                                            cAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCACCGGCCCCTGCGCCATTGCATTGGGCGGACTGTTTTAAAGCGGGGTGCCGATGCCGCCGGCg                                                                            >  1:1359074/1‑151 (MQ=255)                                                                             aGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTGATATTTCGTTTTGTTCGCCCATCGGCCTCCGCGCCATTTCCTTTGGGGTACTGTTTTAAAGGGGGGTGGCGAGGCCGCTGGCgg                                                                           >  2:681601/1‑150 (MQ=255)                                                                               tGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTTGACCATCGGCCTCTGCGCCATTGCATTGGGGGGACTGTTTCAAAGAGGTGTGCCGATGCCGCCGGCggt                                                                          >  1:146232/1‑148 (MQ=255)                                                                                gACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTTGGCCATCCGCCCCTCCGCCATTGCATTTGGGGTACTGTTTTTAAAGGGGGTGCCGGTGCCGCCGGCggtc                                                                         >  2:835372/1‑147 (MQ=255)                                                                                gACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTTGACCATCGGCCTCTGCGCCATTGCATTGGGCGGACTGTTTCTAAGTGGGGTGCCGATGCCGCCGGCGGTAc                                                                        >  1:1216326/1‑151 (MQ=255)                                                                                gACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGGCCTCTGCGCCATTGCATTTGGCGTACTGTTTTAAAGTGGTGTGCCGATGCCGCTGGCGATAc                                                                        >  1:483733/1‑151 (MQ=255)                                                                                 aCTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCCTTTTGGTCGACCATCGGCCCCTGCGCCATTGCATTGGGGGGACTGTTTCCAAGTGGTGTGCCGATGCCGCTGGCGGTACt                                                                       >  1:1081131/1‑151 (MQ=255)                                                                                  cTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTTGTTTGACCATCGGCCTCTGCGCCCTTGCATTTGGCGGTCTGTTTTTAAATGGGGTGGCGGTGCCGCTGGCGGTACTc                                                                      >  2:1019570/1‑151 (MQ=255)                                                                                     tATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTTGTTCGACCATCGGCCCCTGCGCCATTTCATTTGGGGTACTGTTTTAAAGTGGGGTGCCGATGCCGCTGGCGGTACTCCTg                                                                   >  1:218410/1‑151 (MQ=255)                                                                                        cTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGCTATTTTTTTTTGTTCGGCCATCGGCCCCCGCCCCCCTGGATTTGGCGGACTGTTTTAAAGAGGTGTGCCGGTGCCCCCGGCGCGACTCCCGAcc                                                                >  2:1008697/1‑151 (MQ=255)                                                                                         tGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGCCCATCGGCCTCTGCGCCATTGCATTTGGCGTACTGTTTTTAAGAGGGGTGCCGATGCCGCTGGCGGTACTCCTGACCt                                                               >  2:1340092/1‑151 (MQ=255)                                                                                          gcattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTTGACCATCGGCCTCTGCGCCATTTCATTGGGCGGACTGTTTTCAAGTGGGGTGCCGATGCCGCTGGCGATACTCCTGACCtt                                                              >  1:1164775/1‑151 (MQ=255)                                                                                          gcattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGAc                                                                 <  1:1037587/148‑1 (MQ=255)                                                                                          gcattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCtt                                                              <  1:43772/151‑1 (MQ=255)                                                                                                 cattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTc                                                       <  1:1136406/151‑1 (MQ=255)                                                                                                  attgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGGCCATCGGCCTCCGCGCCATTGCATTGGGCGGACTGTTTCAAAGTGGGGTGCCGGTGCCGCTGGCGGTACTCCTGACCTTTCTGCTCg                                                      >  1:444657/1‑151 (MQ=255)                                                                                                    tgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTGGTATTTTGTTTTGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGGACTGTTTCTAAGTGGGGTGCCGATGCCGCTGGCGGTAATCCTGACCTTTCTGCTCGgc                                                    >  2:609974/1‑151 (MQ=255)                                                                                                    tgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCgg                                                     <  1:43757/150‑1 (MQ=255)                                                                                                        gCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTTGTTTTGTTTGACCCTCGGCCCCTTCGCCCTTGCATTGGGGGTACTGGTTCTAAGTGGGGTGGCGGTGCCGCCGGCGGTACTCCCGACCCTTCTGCTCGgcgcct                                                >  2:1179587/1‑149 (MQ=255)                                                                                                         cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGAACGATATTTTGTTTTGTTCGACCATCGGCCTCTGCGCCCTTTCATTGGGGGGACTGTTTTTTAGTGGGGTGCCGCTGCCGCTTGCGATACTCCTGACCTTACTGCTCGGCGCAtt                                               >  1:784640/1‑151 (MQ=255)                                                                                                          ccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCCTTGCATTGGGCGTACTGTTTTAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATtg                                              >  1:271494/1‑151 (MQ=255)                                                                                                          ccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATtg                                              <  2:172638/151‑1 (MQ=255)                                                                                                           cTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATAATTCGTTTTGTTTGACAATCAGCCTCTGCGGCCTTGGCTTTGGGGGACTGTTTCTAAATGGGGGGGCGCTGCCGCTGGCGGTACTCCTGACCCTAATGCTCGGCGCATtgt                                             >  2:1026382/1‑151 (MQ=255)                                                                                                                gCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGGTTTGTTCGACCATCGGCCTCTGCCCCCTTGCATTGGGCGTACTGTTTTAAAGTGGGGGGCCGGTGCCGCTGGCGGTACTCCTGACCCTACTGCTCGGCGCATTGTGCggg                                        >  2:493233/1‑151 (MQ=255)                                                                                                                 cTAACGATGGTGATTGTCAGTGGCGGGGTCCATATTTTTTTTTGTTTGACCATCCGCCCCTGCGCCCCTTCATTTGGGGGTCTGTTTTAAAATGGGGGGGCCATGCCCCCGGGGTGACCCCTTACCTTAATTCTCGGGGCCTTGTGCgggg                                       >  2:623782/1‑150 (MQ=255)                                                                                                                  tAACGATGGTGATTGTCAGTGGCGGGGTCGCTATTTTTTTTTGTTTTACCAACTGCCCCTTCGCCCCTTCATTTGGGGTTCTGTTTTTAAGTGGGGTGCCGATGCCCCCTGGGATACTCCCGACCCTACTGCTCGGCGCGCTTTGCGGGcg                                      >  2:866369/1‑150 (MQ=255)                                                                                                                   aaCGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGc                                       <  2:1306414/149‑1 (MQ=255)                                                                                                                   aaCGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGc                                       <  2:417173/149‑1 (MQ=255)                                                                                                                     cGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTCGACCACCGGGCCCTGCGCCCTTTCCTTTGGCGGACTGTTTTAAAATGGGGTGCCCGTGCCGCCGGCGGTACCCCTGCCCTTTCTTCTCGGCGCATTGTGCGGGGTggt                                   >  2:405244/1‑149 (MQ=255)                                                                                                                         ggTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCaa                                <  1:772139/150‑1 (MQ=255)                                                                                                                          gTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACg                              <  1:736756/151‑1 (MQ=255)                                                                                                                             aTTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGAACCTCGGGCTCTGCGCCCTTGCATTTGGGGGAATGTTTTTAAGTGGTGTGGCGGTGCCGCCGGGGGTACTCCCGCCCTTTCTTCTCGGCGCATTGTGGGGGCTGGTCAACGCCg                           >  2:98129/1‑151 (MQ=255)                                                                                                                                gTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGat                        <  2:506777/151‑1 (MQ=255)                                                                                                                                  cAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATACCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGatt                       <  2:1245486/150‑1 (MQ=255)                                                                                                                                    gTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGattaat                    <  1:867056/151‑1 (MQ=255)                                                                                                                                      ggCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGattaatta                  <  1:1034473/151‑1 (MQ=255)                                                                                                                                       gCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTAt                 >  1:1214792/1‑151 (MQ=255)                                                                                                                                        cGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATc                >  1:1160772/1‑151 (MQ=255)                                                                                                                                          ggATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCta              <  1:1352086/151‑1 (MQ=255)                                                                                                                                              cGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATAc           <  1:357127/150‑1 (MQ=255)                                                                                                                                               gATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCa         <  1:268928/151‑1 (MQ=255)                                                                                                                                                  atTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCaaa       <  2:1359482/150‑1 (MQ=255)                                                                                                                                                      cGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTaa  <  2:1172265/151‑1 (MQ=255)                                                                                                                                                      cGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTa   >  1:897714/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTAA  >  NC_000913/1604088‑1604387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑