Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,413	T→A	S359R (AGT→AGA)	thrA →	Bifunctional aspartokinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,413	0	T	A	100.0%	279.1 / NA	71	S359R (AGT→AGA)	thrA	Bifunctional aspartokinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (42/29);  total (42/29)

CCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGT  >  NC_000913/1265‑1551                                                                                                                                                     |                                                                                                                                           ccAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGatt                                                                                                                                          <  2:740142/151‑1 (MQ=255)       tGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGt                                                                                                                                    >  2:893529/1‑151 (MQ=255)         aaTAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTc                                                                                                                                  >  2:95850/1‑151 (MQ=255)         aaTAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTc                                                                                                                                  >  1:551674/1‑151 (MQ=255)          aTAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTcc                                                                                                                                 <  1:526929/151‑1 (MQ=255)          aTAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTcc                                                                                                                                 >  1:347096/1‑151 (MQ=255)           tAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTcc                                                                                                                                 >  2:1370935/1‑150 (MQ=255)            aaCATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCcac                                                                                                                               <  1:947204/151‑1 (MQ=255)             aCATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCcac                                                                                                                               >  1:1143362/1‑150 (MQ=255)               aTGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACaa                                                                                                                             >  1:878638/1‑150 (MQ=255)               aTGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACaa                                                                                                                             >  1:1024088/1‑150 (MQ=255)                   cAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGa                                                                                                                        <  2:231815/151‑1 (MQ=255)                        ttCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACtgtg                                                                                                                   >  2:290161/1‑151 (MQ=255)                         tCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACtgtgt                                                                                                                  >  1:62694/1‑151 (MQ=255)                         tCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACtgtg                                                                                                                   <  1:486355/150‑1 (MQ=255)                          cAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACtgtgt                                                                                                                  <  2:887093/150‑1 (MQ=255)                           aGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGc                                                                                                                >  2:1069483/1‑151 (MQ=255)                           aGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGc                                                                                                                <  1:414267/151‑1 (MQ=255)                              gTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAg                                                                                                             <  1:911376/151‑1 (MQ=255)                                    ggTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAAc                                                                                                       <  2:219308/151‑1 (MQ=255)                                        cGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGc                                                                                                   <  2:734190/151‑1 (MQ=255)                                            gATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATg                                                                                               <  1:55564/151‑1 (MQ=255)                                             aTGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc                                                                                              >  2:313023/1‑151 (MQ=255)                                                aaaGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg                                                                                           <  1:378273/151‑1 (MQ=255)                                                aaaGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg                                                                                           >  1:1358391/1‑151 (MQ=255)                                                      aTGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGt                                                                                     <  1:410971/151‑1 (MQ=255)                                                      aTGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGt                                                                                     <  1:1267091/151‑1 (MQ=255)                                                             gCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCt                                                                              <  1:1119660/151‑1 (MQ=255)                                                                tGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTgg                                                                            >  1:1045204/1‑150 (MQ=255)                                                                     gcgcgcGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTg                                                                       >  1:121735/1‑150 (MQ=255)                                                                     gcgcgcGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGa                                                                      <  1:966134/151‑1 (MQ=255)                                                                      cgcgcgTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGa                                                                      >  2:296950/1‑150 (MQ=255)                                                                       gcgcgTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGaa                                                                     >  2:1018404/1‑150 (MQ=255)                                                                        cgcgTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAaag                                                                   <  2:879370/151‑1 (MQ=255)                                                                          cgTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAaagaa                                                                 <  2:11035/151‑1 (MQ=255)                                                                             cTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGc                                                              >  2:289633/1‑151 (MQ=255)                                                                               ttGCAGCGATGTCACGCGCCCGTATTGCCGTGGTTCTGATTGCGCAATCATCTTCCGAATACAGCAACAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACTGGCAATGCAGGAAGAGTGCTACCTGGAACTGAAAGAAGGCt                                                             <  2:711008/150‑1 (MQ=255)                                                                                  cAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACt                                                         <  1:296904/151‑1 (MQ=255)                                                                                      gATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGACAGAAGGCTTACTGGac                                                     >  2:237026/1‑150 (MQ=255)                                                                                       aTGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGc                                                    >  2:333814/1‑151 (MQ=255)                                                                                       aTGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGc                                                    <  1:1080293/151‑1 (MQ=255)                                                                                         gTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCg                                                  <  1:457232/151‑1 (MQ=255)                                                                                         gTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCg                                                  <  2:170700/151‑1 (MQ=255)                                                                                              gcgcCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGc                                             >  1:990389/1‑151 (MQ=255)                                                                                              gcgcCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGc                                             <  1:549363/151‑1 (MQ=255)                                                                                                  ccGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTg                                         >  2:516705/1‑151 (MQ=255)                                                                                                      aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACg                                      <  2:912089/150‑1 (MQ=255)                                                                                                       tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACgg                                     >  1:222481/1‑150 (MQ=255)                                                                                                        ttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGa                                    >  2:446277/1‑150 (MQ=255)                                                                                                         tCCGTGGTGCTGATTACGCAATCGTCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAAc                                  >  1:446044/1‑151 (MQ=255)                                                                                                          ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAAc                                  <  2:895172/150‑1 (MQ=255)                                                                                                              ggtgCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACggat                              <  2:551654/150‑3 (MQ=255)                                                                                                              ggtgCTGATTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTg                             >  1:226059/1‑151 (MQ=255)                                                                                                                     aTTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCAtt                       >  1:385933/1‑150 (MQ=255)                                                                                                                     aTTACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCAtt                       >  2:702134/1‑150 (MQ=255)                                                                                                                       tACGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTAt                     <  1:95853/150‑1 (MQ=255)                                                                                                                         cGCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTAtctc                  >  2:1356889/1‑151 (MQ=255)                                                                                                                          gCAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCg                 >  2:874173/1‑151 (MQ=255)                                                                                                                           cAATCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCgg                >  1:587486/1‑151 (MQ=255)                                                                                                                            aaTCATCTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCggt               >  1:354239/1‑151 (MQ=255)                                                                                                                               catcTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCggtgg             <  1:773365/150‑1 (MQ=255)                                                                                                                                atcTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCggtggt            >  1:1092261/1‑150 (MQ=255)                                                                                                                                 tcTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGGACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCggtggt            >  1:1277815/1‑149 (MQ=255)                                                                                                                                 tcTTCCGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCggtggt            >  1:230933/1‑149 (MQ=255)                                                                                                                                     ccGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTg       <  1:794239/150‑1 (MQ=255)                                                                                                                                      cGAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGAt     >  1:790779/1‑151 (MQ=255)                                                                                                                                       gAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATg    >  2:284968/1‑151 (MQ=255)                                                                                                                                       gAATACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGCGATg    >  1:847335/1‑151 (MQ=255)                                                                                                                                        aaTACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATgg   >  2:121825/1‑151 (MQ=255)                                                                                                                                         aTACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGt  >  2:226195/1‑151 (MQ=255)                                                                                                                                         aTACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGt  >  2:151735/1‑151 (MQ=255)                                                                                                                                          tACAGCATCAGATTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGt  >  1:519021/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                           CCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGT  >  NC_000913/1265‑1551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑