Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,514,455	A→G	I202T (ATC→ACC)	rpe ←	D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,514,455	0	A	G	100.0%	268.9 / NA	80	I202T (ATC→ACC)	rpe	D‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/36);  total (44/36)

AGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGT  >  NC_000913/3514309‑3514600                                                                                                                                                   |                                                                                                                                                  agcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGc                                                                                                                                               <  1:762869/151‑1 (MQ=255)  gcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGcc                                                                                                                                              >  1:1108116/1‑151 (MQ=255)  gcagcaAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGcc                                                                                                                                              <  1:1361399/151‑1 (MQ=255)       aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAAcc                                                                                                                                         >  1:349947/1‑151 (MQ=255)       aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAAcc                                                                                                                                         >  1:698342/1‑151 (MQ=255)       aaGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAAcc                                                                                                                                         <  2:378328/151‑1 (MQ=255)        aGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCg                                                                                                                                        <  2:144501/151‑1 (MQ=255)        aGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCg                                                                                                                                        <  2:1197484/151‑1 (MQ=255)        aGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCg                                                                                                                                        >  2:235056/1‑151 (MQ=255)              ggAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGcgacg                                                                                                                                  <  1:1203944/151‑1 (MQ=255)               gAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGcgacga                                                                                                                                 <  2:1306119/151‑1 (MQ=255)                      gTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACatat                                                                                                                           <  2:1119541/150‑1 (MQ=255)                           ccAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCgcgc                                                                                                                     <  2:377533/151‑1 (MQ=255)                                 tACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCccgccg                                                                                                               >  1:1285147/1‑151 (MQ=255)                                   ccATCAAGATAAAAAGCGCCGCCGCAAATATCTTCAAACTTATTCATGCCTTCCCTTTGCCATTTCACTGCGCATTTCATCAATGCCTTTTTTGTGGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACAAACATATCCGCGCCCGCCGCa                                                                                                             <  2:217187/151‑1 (MQ=255)                                     aTCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGc                                                                                                           >  1:103553/1‑151 (MQ=255)                                     aTCAAGATCAAAAGCGACGCCGCAAATATCTACAAACTTATTCATGACTTCCCTTTGCCATTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGCCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGc                                                                                                           <  2:95529/151‑1 (MQ=255)                                           aTCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTc                                                                                                     <  1:605326/151‑1 (MQ=255)                                            tCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCg                                                                                                    >  2:1044324/1‑151 (MQ=255)                                              aaaaGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGcc                                                                                                  >  2:1159913/1‑151 (MQ=255)                                                aaGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCa                                                                                                 >  2:877432/1‑150 (MQ=255)                                                     aCGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgttg                                                                                           >  1:1213177/1‑151 (MQ=255)                                                      cgccgcGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgttgt                                                                                          >  1:317550/1‑151 (MQ=255)                                                      cgccgcGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAAtgttgt                                                                                          <  1:1300308/151‑1 (MQ=255)                                                        ccgcGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTc                                                                                        <  1:1113926/151‑1 (MQ=255)                                                            gAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCCCTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTG‑CGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCtt                                                                                   <  1:376392/151‑1 (MQ=255)                                                             aaTATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCtt                                                                                   <  1:1152433/151‑1 (MQ=255)                                                                  cTTCAAACTTATTCCTGACTTACCTTTTCCAGTTCACTGCGTATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTTCCGAATTTGTGATGTACTTTTCCTCTCCCTCTTCTTCTATGTCGCCAATGTTGTTCACCTTCACGc                                                                              >  1:952467/1‑151 (MQ=255)                                                                  cTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGc                                                                              >  2:502687/1‑151 (MQ=255)                                                                   ttCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGcc                                                                             >  1:283623/1‑151 (MQ=255)                                                                    tCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCAc                                                                                <  1:1079390/147‑1 (MQ=255)                                                                          ttATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTcc                                                                      >  1:406312/1‑151 (MQ=255)                                                                            aTTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCAc                                                                    >  1:309415/1‑151 (MQ=255)                                                                            aTTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCAc                                                                    >  1:8572/1‑151 (MQ=255)                                                                            aTTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCAAATGTTGTTCACCTTCACGCCACCGTCCAc                                                                    >  2:211584/1‑151 (MQ=255)                                                                             ttCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACt                                                                   >  1:641390/1‑151 (MQ=255)                                                                               cATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACtt                                                                  <  1:542239/150‑1 (MQ=255)                                                                                  gACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCt                                                                >  2:912476/1‑149 (MQ=255)                                                                                       aCCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGa                                                          >  1:677056/1‑150 (MQ=255)                                                                                        ccTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAAt                                                        >  2:208105/1‑151 (MQ=255)                                                                                         cTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATg                                                       >  2:622226/1‑151 (MQ=255)                                                                                            tGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTc                                                     <  1:1014150/150‑1 (MQ=255)                                                                                               cAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAg                                                 <  1:1067865/151‑1 (MQ=255)                                                                                                aGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGc                                                >  1:1290187/1‑151 (MQ=255)                                                                                                 gTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGcc                                               >  1:485248/1‑151 (MQ=255)                                                                                                   tCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGcc                                               >  2:774826/1‑149 (MQ=255)                                                                                                   tCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCa                                              <  1:672091/150‑1 (MQ=255)                                                                                                      cTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACt                                          >  1:674891/1‑151 (MQ=255)                                                                                                        gcgcATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACtcg                                        >  2:501854/1‑151 (MQ=255)                                                                                                        gcgcATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACt                                          <  2:1122378/149‑1 (MQ=255)                                                                                                         cgcATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGACCCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACtcgt                                       <  2:762743/151‑1 (MQ=255)                                                                                                          gcATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACtcgtc                                      >  2:1218228/1‑151 (MQ=255)                                                                                                          ccATTTCATCAATGCCTTTTTTGTAGTCTGGCTGGTGGAAGGTGGCCGAACCGGCGACGAACATACCCGCCCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACtcgtc                                      <  2:1237597/150‑1 (MQ=255)                                                                                                           cATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACtcgtcg                                     >  1:201834/1‑151 (MQ=255)                                                                                                              ttCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGAt                                   >  2:581013/1‑150 (MQ=255)                                                                                                              ttCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGAt                                   >  2:801246/1‑150 (MQ=255)                                                                                                               tcatcaATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATAc                                 >  1:1199719/1‑151 (MQ=255)                                                                                                                catcaATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACg                                <  1:560170/151‑1 (MQ=255)                                                                                                                   caATGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGc                              <  2:479635/150‑1 (MQ=255)                                                                                                                    aaTGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCg                             >  1:1340759/1‑150 (MQ=255)                                                                                                                     aTGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGAc                           >  2:290460/1‑151 (MQ=255)                                                                                                                     aTGACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGAc                           >  2:413538/1‑151 (MQ=255)                                                                                                                       gACTTTTTTGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACg                          <  2:406296/150‑1 (MQ=255)                                                                                                                           tttttGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACt                      <  1:1276367/150‑1 (MQ=255)                                                                                                                             tttGTAGTCTGGCTGGTCGAAGGTTGCCGACCCGGCGACGAACATTCCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTc                    <  2:416093/150‑1 (MQ=255)                                                                                                                             tttGTAGTCTGGCTGGTCGAAGGTTGCCGACCCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTc                    <  2:876987/150‑1 (MQ=255)                                                                                                                              ttGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTcgc                  >  1:301240/1‑151 (MQ=255)                                                                                                                              ttGTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTcgc                  >  2:57551/1‑151 (MQ=255)                                                                                                                                gTAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTcgcg                 <  2:1357965/150‑1 (MQ=255)                                                                                                                                 tAGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCgcgc                >  1:987901/1‑150 (MQ=255)                                                                                                                                  aGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCgcgc                >  2:464074/1‑149 (MQ=255)                                                                                                                                  aGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCgcgc                >  2:83860/1‑149 (MQ=255)                                                                                                                                  aGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCgcgc                >  1:742866/1‑149 (MQ=255)                                                                                                                                  aGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCa               <  2:930392/150‑1 (MQ=255)                                                                                                                                  aGTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAg              <  1:143166/151‑1 (MQ=255)                                                                                                                                   gTCTGGCTGGTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGt             >  1:612882/1‑151 (MQ=255)                                                                                                                                       ggctggTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTAt         <  1:867666/151‑1 (MQ=255)                                                                                                                                           ggTCGAAGGTTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATcc       >  2:1013103/1‑149 (MQ=255)                                                                                                                                           ggTCAAAGGTTGCCGACCCGGCGACGAACATACCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAg     <  2:964992/151‑1 (MQ=255)                                                                                                                                              ggAAGGTTGCCGACCCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAgtgt  <  1:550702/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  AGCAGCAAGACCAGGAGCACTGTCGACCAGCGTACCATCAAGATCAAAAGCGACGCCGCGAATATCTTCAAACTTATTCATGACTTACCTTTGCCAGTTCACTGCGCATTTCATCAATGACTTTTTTGTAGTCTGGCTGGTCGAAGATTGCCGAACCGGCGACGAACATATCCGCGCCCGCCGCAGCGATTTCGCCAATGTTGTTCACCTTCACGCCACCGTCCACTTCTAGTCGAATGTCAAAGCCAGACTCGTCGATACGGCGACGTACTTCGCGCAGTTTATCCAGTGT  >  NC_000913/3514309‑3514600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑