Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913366,5260TG59.3% 26.6 / 36.9 27T329P (ACC→CCC) lacIlactose‑inducible lac operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/11);  new base G (16/0);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 7.67e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.18e-06

AGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGC  >  NC_000913/366379‑366675
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aGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCAggggggg                                                                                                                                                     >  2:365868/1‑150 (MQ=255)
 gCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCCGGGGGGtt                                                                                                                                                    >  2:60315/1‑151 (MQ=255)
     ggggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCAGGGGGGgttttt                                                                                                                                                >  2:178724/1‑150 (MQ=255)
      gggTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGCGCCAGGGGGGttttt                                                                                                                                                 >  2:106567/1‑149 (MQ=255)
         tGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGGGCAGGGGGGTttttctttt                                                                                                                                            >  1:100357/1‑151 (MQ=255)
          gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGGGCAGGGGGGGtttttttttt                                                                                                                                           >  2:187071/1‑150 (MQ=255)
           ccTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGCATCGGCCAACGCGCGGGGAGAGGCGGATTTCGGCGTGAACGCCAGGGGGGGGTTTTTTTTCa                                                                                                                                          >  1:43848/1‑151 (MQ=255)
              aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGGGGTTTTTTTTTTTACCa                                                                                                                                       >  2:296763/1‑151 (MQ=255)
                 gagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCCGGGGGGGTTTTTTTTTCACCAGTg                                                                                                                                    >  2:375759/1‑151 (MQ=255)
                      agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGCGGCCGGGGGGGTTTTCTTTTTACCCGTGAGGCg                                                                                                                               >  1:438517/1‑151 (MQ=255)
                                caTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGGGGGTTTTCTTTTTACCAGTGAGACGGGCAACAg                                                                                                                       >  2:282943/1‑149 (MQ=255)
                                   tAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTGGGCGCCCGGGGGGGTTTTTTTTTTACCCGGGAGGCGGGCAACAGCG‑GAt                                                                                                                 >  1:148832/1‑151 (MQ=255)
                                    aaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GAtt                                                                                                                <  1:156369/151‑1 (MQ=255)
                                              cgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCAc                                                                                                       <  2:448133/150‑1 (MQ=255)
                                                    cTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGGGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTgg                                                                                                >  2:41107/1‑151 (MQ=255)
                                                       cccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGccc                                                                                             <  1:258473/151‑1 (MQ=255)
                                                             ttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTgagag                                                                                       <  1:311971/151‑1 (MQ=255)
                                                             ttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTgagag                                                                                       <  2:386361/151‑1 (MQ=255)
                                                                                    ccAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt                                                                <  1:106006/151‑1 (MQ=255)
                                                                                        cTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGtt                                                            <  2:402018/151‑1 (MQ=255)
                                                                                          gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCGGATTGGGGGCCAGGGGGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCCCCTGGCCCTGAGAGAGTTGCACCAAGCGGTCCACGCTGGGTTg                                                          >  2:204924/1‑151 (MQ=255)
                                                                                          gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGGGGGTTTTCTTTTCACCAGGGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGGTGCAGCAAGCGGGCCACGCTGGTTTg                                                          >  2:60571/1‑151 (MQ=255)
                                                                                             ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGGGTATTGGGGGGCAAGGGGGGTTTTTTTTTCACCAGAGGGAAGGGGAGCAGATGGATGCCCCTCCACCG‑CTGGCCCTGGGGGAGGTGGAGCAAGCGGGCCGCCGTGGGTTGcc                                                        >  1:152087/1‑150 (MQ=255)
                                                                                                  gAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCGCCCGGGGGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagca                                                  >  2:425060/1‑151 (MQ=255)
                                                                                                                             gTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt                        <  1:368938/150‑1 (MQ=255)
                                                                                                                              tttGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTaa                      <  1:209204/151‑1 (MQ=255)
                                                                                                                                  cGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg                   <  2:60426/150‑1 (MQ=255)
                                                                                                                                             ccAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACa       <  1:351325/151‑1 (MQ=255)
                                                                                                                                                 ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACAt      <  1:178721/148‑1 (MQ=255)
                                                                                                                                                   tggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGc  <  1:209721/150‑1 (MQ=255)
                                                                                                                                                   tggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGc  <  1:471888/150‑1 (MQ=255)
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AGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT‑GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGC  >  NC_000913/366379‑366675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑