Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,578,143	0	A	C	53.3%	8.1 / 13.8	15	L79V (TTA→GTA)	maeB	malic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base C (0/8);  total (4/11)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02

AATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAG  >  NC_000913/2578018‑2578293                                                                                                                              |                                                                                                                                                       aaTAAATTTGCCGGGGTCGAGTTCGTCACCTTCAATGTCAAATACACCAACCCCGGCAAATTTCTAAAACAGACCCCCCTTCCCTCCCACCCCCGGTTTGCCTGCCGGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTagag                                                                                                                               <  1:298889/151‑1 (MQ=255)                    atttCGTCACCTTCAATGTAAAATCCATCAACCCCGGCGAATTTCTTAAACAGACCGCCCTCCCCTCCCACCACCGTTTTCCCTGCCAGCGCGCCAATTTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTAc                                                                                                            <  1:108097/149‑1 (MQ=255)                      ttcttcACCTTAAATGTAAAATACACCAACCCCGCCAAATTTCTAAAACAGACCCCCTTTCCCTCCCACCCCCGGTTGCCCTCCAAGCCCCCCATTGTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCt                                                                                                          <  1:397562/151‑1 (MQ=255)                                 aaaTGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtata                                                                                               <  2:266903/150‑1 (MQ=255)                                     ttaacttccATCACCCCCCGCAATTTTCTAAAAGAGCCCCCCCTTGCCTTCCATCCCCGTTCTGCCTGCCAGCGCCCCAATGTTCCCTACCCCCAGCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAGTTg                                                                                           <  1:295384/143‑1 (MQ=255)                                     ttaaaaTACATCAACCCCGGCAAATTTCTAAAACAAAACGCCCTTCCCTTCCATCCCCGTTTTGCCTGCCAGCGCGCAAATTTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg                                                                                           <  2:461880/148‑1 (MQ=255)                                     gTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg                                                                                           >  1:403285/1‑151 (MQ=255)                                     gTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg                                                                                           >  2:193348/1‑151 (MQ=255)                                      taaaaTACATCAACCCCGGCGAATTTCTTAAACAGACCGCCCTTGCCTTCCATCCCCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg                                                                                           <  2:170618/148‑1 (MQ=255)                                                                   aaaCAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa                                                             >  1:82571/1‑151 (MQ=255)                                                                                              cGGTTCGCCTGCCAGCGCGCCAATGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGCGAg                                  <  1:374921/151‑1 (MQ=255)                                                                                                      ctgccAGCGCGCAAATGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAg                          <  2:98566/151‑1 (MQ=255)                                                                                                              ccgcCAATGTCCCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGAt                  <  1:80707/150‑1 (MQ=255)                                                                                                              cccccAATGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGAt                  <  1:214038/148‑1 (MQ=255)                                                                                                                        tGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTgtgt        >  1:422145/1‑151 (MQ=255)                                                                                                                              aCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCag  >  2:391223/1‑151 (MQ=255)                                                                                                                              |                                                                                                                                                       AATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCCAG  >  NC_000913/2578018‑2578293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑