Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,364	A→T	L193Q (CTG→CAG)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,364	0	A	T	100.0%	128.0 / NA	34	L193Q (CTG→CAG)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/22);  total (12/22)

GTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAG  >  NC_000913/2907233‑2907510                                                                                                                                    |                                                                                                                                                   gTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGa                                                                                                                                 >  1:165672/1‑151 (MQ=255)   tCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAc                                                                                                                               <  2:370977/151‑1 (MQ=255)     aTAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAccc                                                                                                                             <  1:464346/151‑1 (MQ=255)               gcTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTTGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAc                                                                                                                               >  2:44981/1‑139 (MQ=255)               gcTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTTGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAAc                                                                                                                               <  1:44981/139‑1 (MQ=255)                             tgCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGtt                                                                                                     >  2:153809/2‑151 (MQ=255)                              gCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGttt                                                                                                    >  2:395185/1‑151 (MQ=255)                                 aTAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTcg                                                                                                 >  2:406888/1‑151 (MQ=255)                                     cAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCgcgc                                                                                              >  1:295543/1‑150 (MQ=255)                                           gagCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCgg                                                                                       >  2:342407/1‑151 (MQ=255)                                             gCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCgg                                                                                       >  1:204139/1‑149 (MQ=255)                                                                 gTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTgg                                                                    <  2:305123/148‑1 (MQ=255)                                                                      gcgcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCaag                                                            >  2:419111/1‑150 (MQ=255)                                                                       cgcATAACCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCaagg                                                           <  2:309982/151‑3 (MQ=255)                                                                        gcATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTCCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGgt                                                          >  1:189396/1‑151 (MQ=255)                                                                               cACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTc                                                   <  1:244383/151‑1 (MQ=255)                                                                               cACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTc                                                   <  2:112258/151‑1 (MQ=255)                                                                                 ccTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAg                                                 <  1:342398/151‑1 (MQ=255)                                                                                     cGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTg                                             <  2:463657/151‑1 (MQ=255)                                                                                         aCCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTca                                         >  2:369300/1‑151 (MQ=255)                                                                                           cAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTcac                                        >  2:9462/1‑150 (MQ=255)                                                                                                 cagTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTcaccac                                     <  2:365698/147‑1 (MQ=255)                                                                                                    tgtTCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtg                                <  2:277953/149‑1 (MQ=255)                                                                                                    tgtTCATGCCTTTCGCTTTCAGAACGTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtg                                <  2:19051/149‑1 (MQ=255)                                                                                                      ttCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtgatga                            <  1:219518/151‑1 (MQ=255)                                                                                                      ttCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtgatga                            <  2:183057/151‑1 (MQ=255)                                                                                                      ttCATGCCTTTCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTtgatga                            <  2:71531/151‑1 (MQ=255)                                                                                                              tttCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtcatc                    <  2:330468/151‑1 (MQ=255)                                                                                                              tttCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTtcatc                    <  2:125452/151‑1 (MQ=255)                                                                                                                tCGCTTTCAGAACTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTcatcat                  <  2:434022/151‑1 (MQ=255)                                                                                                                            cTTTTGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCAt      <  1:436849/151‑1 (MQ=255)                                                                                                                                tGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATagag  <  2:349655/151‑1 (MQ=255)                                                                                                                                tGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATagag  <  2:400468/151‑1 (MQ=255)                                                                                                                                tGCCTGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAAGGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATagag  <  2:143800/151‑1 (MQ=255)                                                                                                                                    |                                                                                                                                                   GTTCATAACCAGCAGCTTTAACAGCTTCAGCGATAACAGCCAGAGCTTCAGCGTTGGAACCCAGGTTCGGCGCATAGCCACCTTCGTCACCAACAGCAGTGTTCATGCCTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAG  >  NC_000913/2907233‑2907510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑