Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,060,146	C→A	Q132K (CAG→AAG)	argP →	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,060,146	0	C	A	100.0%	75.3 / NA	21	Q132K (CAG→AAG)	argP	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/11);  total (10/11)

ATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCC  >  NC_000913/3060000‑3060271                                                                                                                                                   |                                                                                                                              aTGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAgg                                                                                                                            <  2:463344/150‑1 (MQ=255) aTGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAgg                                                                                                                            <  1:214146/150‑1 (MQ=255)             gTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGcgccg                                                                                                               >  2:335617/1‑151 (MQ=255)                      cGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGt                                                                                                      >  1:196258/1‑151 (MQ=255)                        ctgctgctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTgg                                                                                                    <  2:356976/151‑1 (MQ=255)                               tttCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGgcg                                                                                              >  2:374132/1‑150 (MQ=255)                                           tCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTc                                                                                  <  1:36680/150‑1 (MQ=255)                                               cGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCCCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACAt                                                                             <  2:169088/151‑1 (MQ=255)                                                        tCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGgc                                                                        <  1:374122/147‑1 (MQ=255)                                                            gCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGa                                                                >  1:324002/1‑151 (MQ=255)                                                               aCGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGt                                                              <  2:410538/150‑1 (MQ=255)                                                                       gCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGttgtcttgt                                                      >  1:424140/1‑150 (MQ=255)                                                                              gCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATaaa                                               >  1:105818/1‑150 (MQ=255)                                                                                   ggCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGt                                         >  2:146993/1‑151 (MQ=255)                                                                                          gtgtTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCg                                  >  2:129339/1‑151 (MQ=255)                                                                                          gtgtTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCg                                  >  1:240763/1‑151 (MQ=255)                                                                                             ttGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGAc                                <  1:461155/150‑1 (MQ=255)                                                                                                  tGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAAAGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTg                          <  1:262499/151‑1 (MQ=255)                                                                                                       cGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGt                     >  1:337429/1‑151 (MQ=255)                                                                                                                         tGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGc   <  2:190417/151‑1 (MQ=255)                                                                                                                           cAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGcc  <  2:172159/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                              ATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCC  >  NC_000913/3060000‑3060271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑