Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,471,274	C→A	G19V (GGC→GTC)	tufA ←	translation elongation factor EF‑Tu 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,471,274	0	C	A	100.0%	166.2 / NA	44	G19V (GGC→GTC)	tufA	translation elongation factor EF‑Tu 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/22);  total (22/22)

CGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAT  >  NC_000913/3471128‑3471420                                                                                                                                                   |                                                                                                                                                   cgggagaAGTGTTGATGGTGATACCACGGTCTTTTTCTTCCGGCGCGTTATCGATCTGGTGGAATGCTGGGGCAGCACCGGCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGAt                                                                                                                                                <  1:37872/148‑1 (MQ=14)   tGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAg                                                                                                                                              <  2:373929/151‑1 (MQ=255)         gtgtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCaa                                                                                                                                        >  1:39099/1‑151 (MQ=255)          tgtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCa                                                                                                                                         <  1:55359/149‑1 (MQ=255)                                                               aaTGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTc                                                                                   <  1:95024/150‑1 (MQ=255)                                                                 tGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCtt                                                                                >  2:227584/1‑151 (MQ=255)                                                                  gCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTACCGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTa                                                                               >  1:318673/1‑151 (MQ=255)                                                                         cagcaCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAg                                                                         <  1:216088/150‑1 (MQ=255)                                                                           gcaCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTg                                                                       >  2:257662/1‑150 (MQ=255)                                                                            caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  2:246043/150‑1 (MQ=255)                                                                            caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  1:350843/150‑1 (MQ=255)                                                                            caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  1:385267/150‑1 (MQ=255)                                                                                 ccgTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTccc                                                                <  1:153590/151‑1 (MQ=255)                                                                                       gTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAg                                                          >  2:81495/1‑151 (MQ=255)                                                                                        ttttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                         <  2:10040/151‑1 (MQ=255)                                                                                        ttttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                         <  1:322682/151‑1 (MQ=255)                                                                                          ttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                       <  1:438736/151‑1 (MQ=255)                                                                                          ttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                       <  2:239059/151‑1 (MQ=255)                                                                                          ttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgag                                                       <  1:65838/151‑1 (MQ=255)                                                                                                 gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                 >  1:107537/1‑150 (MQ=255)                                                                                                 gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                 >  2:217978/1‑150 (MQ=255)                                                                                                 gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                 >  1:403328/1‑150 (MQ=255)                                                                                                  tACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGc                                                  <  1:277598/148‑1 (MQ=255)                                                                                                      gtggtgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGa                                            >  2:189334/1‑150 (MQ=255)                                                                                                          tgATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTg                                       >  2:463589/1‑151 (MQ=255)                                                                                                              tGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTAt                                   <  1:276040/151‑1 (MQ=255)                                                                                                                   cGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAcc                              <  1:282159/151‑1 (MQ=255)                                                                                                                   cGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAc                               <  2:186801/150‑1 (MQ=255)                                                                                                                         gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  1:53257/1‑151 (MQ=255)                                                                                                                         gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  1:215788/1‑151 (MQ=255)                                                                                                                         gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  2:359365/1‑151 (MQ=255)                                                                                                                         gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  2:289214/1‑151 (MQ=255)                                                                                                                         gagTAGGTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  1:7713/1‑151 (MQ=255)                                                                                                                          agtagtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAg                         <  2:180527/149‑1 (MQ=255)                                                                                                                           gtagtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  2:63220/1‑149 (MQ=255)                                                                                                                             agtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTa                    >  2:315933/1‑151 (MQ=255)                                                                                                                                    ccGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCa             <  1:467251/151‑1 (MQ=255)                                                                                                                                      gTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACg           >  2:163440/1‑151 (MQ=255)                                                                                                                                        ggTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg         >  2:242502/1‑151 (MQ=255)                                                                                                                                             aCGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCa    >  2:151637/1‑151 (MQ=255)                                                                                                                                              cGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGCGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCaa   >  2:321844/1‑151 (MQ=255)                                                                                                                                               gTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAt  >  2:166235/1‑151 (MQ=255)                                                                                                                                                tGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAt  <  2:355305/150‑1 (MQ=255)                                                                                                                                                tGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAt  <  1:238281/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAT  >  NC_000913/3471128‑3471420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑