Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	933,022	A→G	D143G (GAC→GGC)	lrp →	leucine‑responsive global transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	933,022	0	A	G	100.0%	77.5 / NA	22	D143G (GAC→GGC)	lrp	leucine‑responsive global transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

CGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACC  >  NC_000913/932879‑933164                                                                                                                                                |                                                                                                                                               cGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACgg                                                                                                                                         <  1:389513/151‑1 (MQ=255)  gCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGa                                                                                                                                        >  1:236460/1‑151 (MQ=255)   cTGTAGAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACAACCTGTTGAAAACACGCGTTCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAACCCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGa                                                                                                                                        <  1:229183/150‑1 (MQ=255)              cTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTa                                                                                                                            <  2:147871/151‑1 (MQ=255)              cTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTa                                                                                                                            <  2:102158/151‑1 (MQ=255)                     aaaTTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGgaaga                                                                                                                     <  2:36903/151‑1 (MQ=255)                     aaaTTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGgaaga                                                                                                                     >  1:356298/1‑151 (MQ=255)                       aTTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGaagaag                                                                                                                   >  1:148239/1‑151 (MQ=255)                                                    tttCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAg                                                                                      <  1:342468/151‑1 (MQ=255)                                                              cTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTaa                                                                             >  2:144978/1‑150 (MQ=255)                                                                  tGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACgg                                                                        <  1:125379/151‑1 (MQ=255)                                                                                ccGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAAt                                                          <  1:258827/151‑1 (MQ=255)                                                                                      atGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGt                                                    >  1:446104/1‑151 (MQ=255)                                                                                        gTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTAt                                                  >  1:211886/1‑151 (MQ=255)                                                                                                aCCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTa                                          >  1:400580/1‑151 (MQ=255)                                                                                                           cTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTa                               <  1:156661/151‑1 (MQ=255)                                                                                                               gggAAACCCTGCTGCGTCTGCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATc                            >  1:96880/1‑150 (MQ=255)                                                                                                                                  gCCTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTggg        >  1:212204/1‑151 (MQ=255)                                                                                                                                   ccTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTgggg       <  1:249755/151‑1 (MQ=255)                                                                                                                                   ccTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTgggg       <  1:392186/151‑1 (MQ=255)                                                                                                                                   ccTGGCGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTgggg       <  2:223661/151‑1 (MQ=255)                                                                                                                                        cGTCAATGGCACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAAcc  <  1:445153/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               CGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTATTTTGATTACACTCCTGTTAATCCATACAGCAACAGTACTGGGGTAACC  >  NC_000913/932879‑933164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑