Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,818,103	A→T	V133E (GTA→GAA)	chbF ←	phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,818,103	0	A	T	100.0%	73.9 / NA	20	V133E (GTA→GAA)	chbF	phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/14);  total (6/14)

CACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAG  >  NC_000913/1817986‑1818247                                                                                                                      |                                                                                                                                                 cacacGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGAc                                                                                                                 <  2:227511/151‑1 (MQ=255)       ccGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaa                                                                                                           <  2:178498/151‑1 (MQ=255)             aaGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTaaacaaac                                                                                                     <  1:304164/151‑1 (MQ=255)                 gTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAAccgc                                                                                                  >  2:85155/1‑150 (MQ=255)                  tttAAATCCGGTAGGACGATAAACGGCTTCATTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAAccgcc                                                                                                 <  1:342672/150‑1 (MQ=255)                            gTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTgg                                                                                      >  2:84906/1‑151 (MQ=255)                              aTGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGtt                                                                                    <  2:305182/151‑1 (MQ=255)                                  cGATAAAAGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTc                                                                                  >  1:237835/1‑149 (MQ=255)                                                           ccgccgGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATgg                                                       <  1:461996/151‑1 (MQ=255)                                                           ccgccgGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATgg                                                       <  2:26598/151‑1 (MQ=255)                                                           ccgccgGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATgg                                                       <  2:422950/151‑1 (MQ=255)                                                                 ggTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATAc                                                  <  2:56544/150‑1 (MQ=255)                                                                   ttAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGt                                                <  1:84906/150‑1 (MQ=255)                                                                           gTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGt                                       >  1:394482/1‑151 (MQ=255)                                                                                  aCCCATGCATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCAcgcg                                <  1:221770/151‑1 (MQ=255)                                                                                         cATTCGGACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTaa                         <  1:119973/151‑1 (MQ=255)                                                                                               gACAAAGTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGc                    >  2:345282/1‑150 (MQ=255)                                                                                                     gTTCTTCGACATCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAAcgcg             >  2:131238/1‑151 (MQ=255)                                                                                                                tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag  <  1:23116/151‑1 (MQ=255)                                                                                                                tCTTTTTCGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGgtag  <  2:187247/151‑1 (MQ=255)                                                                                                                      |                                                                                                                                                 CACACGCCGATAAAGCGTTTAAATCCGGTATGACGATAAACGGCTTCAGTGACCATTCCCGCCGGGTTAGTGAAGTTAATCACCCATGCATTCGGACAAAGTTCTTCGACATCTTTTACGATGTCAAAAATCACCGGAATGGTACGCAGACCTTTAAACAAACCGCCCGCGCCGTTGGTTTCCTGACCAAGATAACCATGACTTAATGGAATACGTTCATCCAGTTCACGCGCCGGTAATTGGCCAACGCGCAGTTGGGTAG  >  NC_000913/1817986‑1818247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑