Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,492,323	IS1 (–) +9 bp	coding (924‑932/1251 nt)	frc ←	formyl‑CoA transferase, NAD(P)‑binding

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	37 (0.800)	30/296	0.3	97.2%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 2492331	1 (0.020)					coding (924/1251 nt)	frc	formyl‑CoA transferase, NAD(P)‑binding
*	?	NC_000913	= 1979270	NA (NA)	46 (0.990)	31/296	0.2	97.7%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	2492323 =	1 (0.020)					coding (932/1251 nt)	frc	formyl‑CoA transferase, NAD(P)‑binding

TCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCA  >  NC_000913/1978483‑1978633                     |                                                                                                                                   gggacgaagatgggtAAATGGGCAATGACTCAAACTTATTGATAGTGTTTTATGTTGAGATAATGTCCGATGACTATGTCATGCAGCTCCACCGATTTTGAGAAGGACAGCGACTTCCGTCCCAGTCGTGCCAGGTGCTGCCTCAGATTCa  >  1:95589/15‑151 (MQ=11)                     |                                                                                                                                   TCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCA  >  NC_000913/1978483‑1978633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.