New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4409625 =	59 (1.270)	6 (0.140)	5/276	3.0	10.5%	coding (351/732 nt)	rlmB	23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
	?	NC_000913	4409708 =	47 (1.090)					coding (434/732 nt)	rlmB	23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

CCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGACCGGACGCCTGGCGCTGG  >  NC_000913/4409562‑4409854                                                                                                                                                   |                                                                                                                                                   ccTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCa                                                                                                                                                >  2:22738/1‑151 (MQ=255)       gATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAg                                                                                                                                           >  1:362696/1‑150 (MQ=255)        aTCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCg                                                                                                                                         >  2:202719/1‑151 (MQ=255)           cTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTc                                                                                                                                      <  1:167009/151‑1 (MQ=255)                     tAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCg                                                                                                                            >  1:356363/1‑151 (MQ=255)                               gCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTaa                                                                                                                   >  2:266086/1‑150 (MQ=255)                               gCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTaa                                                                                                                   >  2:593137/1‑150 (MQ=255)                                cacaACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAAcc                                                                                                                 <  1:165631/151‑1 (MQ=255)                                 acaACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCt                                                                                                                <  2:561850/151‑1 (MQ=255)                                 acaACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCt                                                                                                                <  2:9841/151‑1 (MQ=255)                                    aCCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACACTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTg                                                                                                              >  2:261221/1‑150 (MQ=255)                                     ccTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                                                            <  1:649841/151‑1 (MQ=255)                                     ccTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                                                            <  1:622622/151‑1 (MQ=255)                                     ccTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTgcg                                                                                                            <  1:301037/151‑1 (MQ=255)                                           cgcgTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCAc                                                                                                       <  2:611827/150‑1 (MQ=255)                                               tGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATg                                                                                                   >  1:344049/1‑150 (MQ=255)                                               tGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATg                                                                                                   >  1:669873/1‑150 (MQ=255)                                                 ccTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCg                                                                                                 <  1:446214/150‑1 (MQ=255)                                                 ccTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCg                                                                                                 <  1:698570/150‑1 (MQ=255)                                                  cTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTa                                                                                               <  2:503066/151‑1 (MQ=255)                                                   tGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTAt                                                                                              >  1:669344/1‑151 (MQ=255)                                                     cGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATg                                                                                             <  1:595486/150‑1 (MQ=255)                                                     cGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGt                                                                                            <  1:488082/151‑1 (MQ=255)                                                     cGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGt                                                                                            <  1:347374/151‑1 (MQ=255)                                                     cGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGt                                                                                            <  1:675759/151‑1 (MQ=255)                                                     cGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGt                                                                                            <  1:353050/151‑1 (MQ=255)                                                         gTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCa                                                                                        >  2:269734/1‑151 (MQ=255)                                                              gACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAag                                                                                   >  2:78160/1‑151 (MQ=255)                                                               aCGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAag                                                                                   <  1:11838/150‑1 (MQ=255)                                                                           ttCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAt                                                                      <  1:135239/151‑1 (MQ=255)                                                                           ttCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAt                                                                      <  2:619469/151‑1 (MQ=255)                                                                           ttCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAt                                                                      <  2:295641/151‑1 (MQ=255)                                                                               tGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGAtcgtc                                                                  <  1:367487/151‑1 (MQ=255)                                                                                                      gCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCa                                           >  1:262364/1‑151 (MQ=255)                                                                                                      gCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCa                                           >  2:275392/1‑151 (MQ=255)                                                                                                           gCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATaca                                       >  1:50902/1‑150 (MQ=255)                                                                                                            cacaGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATacac                                      <  1:329333/150‑1 (MQ=255)                                                                                                                gCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTAt                                 >  2:448966/1‑151 (MQ=255)                                                                                                                 cTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACActc                                    <  2:75069/147‑1 (MQ=255)                                                                                                                 cTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATc                                <  2:215993/151‑1 (MQ=255)                                                                                                                   cAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCag                              <  1:589760/151‑1 (MQ=255)                                                                                                                   cAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCag                              <  2:597703/151‑1 (MQ=255)                                                                                                                     aCGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCagag                            <  2:236026/151‑1 (MQ=255)                                                                                                                     aCGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCagag                            <  2:296/151‑1 (MQ=255)                                                                                                                              cGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGAc                   <  2:414183/151‑1 (MQ=255)                                                                                                                                       tAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGACCGGACGCCt          <  2:458675/151‑1 (MQ=255)                                                                                                                                       tAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGACCGGACGCCt          <  2:357982/151‑1 (MQ=255)                                                                                                                                          ccTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGACCGGACGCCTgg        <  2:212700/150‑1 (MQ=255)                                                                                                                                            tGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGGCTAGCCTTGCGCGCACCAGGCGTATGTTGGAGGGAGAGAAGATCTGGAGCGTCGGTACGGCAGGGGGGGGGGATCATACACTCTAGCAGAGGAAAATGACCGGACGCCTGgcgg     <  1:664768/151‑2 (MQ=255)                                                                                                                                            tGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGACCGGACGCCTGgcgc     <  1:649056/151‑1 (MQ=255)                                                                                                                                            tGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGACCGGACGCCTGgcgc     <  2:119087/151‑1 (MQ=255)                                                                                                                                                gcgcggcAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGACCGGACGCCTGGCGCTgg  <  2:103129/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGCGCGGCAGAAAGCGTTCCGCTGATTCGGGTGACTAACCTTGCGCGCACCATGCGTATGTTGCAGGAAGAGAATATCTGGATCGTCGGTACGGCAGGCGAGGCGGATCATACACTCTATCAGAGCAAAATGACCGGACGCCTGGCGCTGG  >  NC_000913/4409562‑4409854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.