Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,177,5310AC54.8% 34.6 / 45.3 31V127G (GTG→GGG) nudFADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (8/6);  new base C (0/17);  total (8/23)
Fisher's exact test for biased strand distribution p-value = 3.81e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-07

TGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCC  >  NC_000913/3177383‑3177675
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tGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACAcc                                                                                                                                                <  2:555803/151‑1 (MQ=255)
    tGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGtt                                                                                                                                            <  2:723725/151‑1 (MQ=255)
     gTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGttt                                                                                                                                           >  1:466774/1‑151 (MQ=255)
         ccGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                                                                                                       <  2:677587/151‑1 (MQ=255)
                 ccACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGa                                                                                                                                >  2:675203/1‑150 (MQ=255)
                 ccACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGa                                                                                                                                >  1:165250/1‑150 (MQ=255)
                     aTGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATc                                                                                                                           <  1:651062/151‑1 (MQ=255)
                          cgcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                                                                                                      <  1:368994/151‑1 (MQ=255)
                          cgcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                                                                                                      <  2:218334/151‑1 (MQ=255)
                          cgcgAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTc                                                                                                                       >  2:463910/1‑150 (MQ=255)
                                        ttttCACCAGCCAGCCCGTGAATCCCGCTGGCGGTCGGGGCGCCCCCTCCCCCCCCCATAATTGACGAACGCTCACTGGTGCCCCCCGGGTTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCg                                                                                                        <  1:653920/151‑1 (MQ=255)
                                           tcatcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                                                                                                     >  1:435573/1‑151 (MQ=255)
                                                       ccGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCaa                                                                                         >  2:668353/1‑151 (MQ=255)
                                                            aaTCCCGCTTGCGGTCGTGGCGCCCCCTCCCCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGAAAACTTACCCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCt                                                                                    <  1:171108/151‑1 (MQ=255)
                                                                   ctggCGGTCGGGCCGCCCACTCCCCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGAAAACTTACCCCCGGTTTGGCCCGTTTGACTACCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCacac                                                                             <  1:432549/148‑1 (MQ=255)
                                                                           cggggCGTCCACTCCCCCCCCCATAATTGCCGACCGCTCGCTGGTCCCCCCCGGGCTTGCCAGGAATCTTACCCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCAccc                                                                     <  1:724496/148‑1 (MQ=255)
                                                                           cggggCGCCCCCTCCCCCCACCATAATTGACGACCGCCCCCTGGTCCCCCCCGGCCTTGCCAGAAAACTAACCCCCGGTTTGGTCCGTTTGCCTACCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCAccc                                                                     <  1:306518/148‑1 (MQ=255)
                                                                                ccccccctccccccACCATAATTGCCGAACGCTCCCTGGTGCCCCCCGGGCTTGCCAGTAAACTTAACCCCGGTTTGGCCCGTTTGACTCTCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTc                                                                <  1:354684/140‑1 (MQ=255)
                                                                                   ccccTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAat                                                             <  2:721696/148‑1 (MQ=255)
                                                                                     cctccccccACAATAATGGACGACCGCTCCCTGGTCCCCCCCGGGCTTGCCAGAAATCTTACCCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatca                                                           <  1:442682/145‑1 (MQ=255)
                                                                                     ccTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAatca                                                           <  2:266615/150‑1 (MQ=255)
                                                                                                    aTTGACGACCGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCAtct                                            <  2:685373/151‑1 (MQ=255)
                                                                                                             cGCTCCCTGGTCCCCCCCGGCCTTGCAAGAAAACTAAACCCCGTTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGcc                                   <  1:371842/151‑1 (MQ=255)
                                                                                                              gCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCa                                  >  1:12285/1‑151 (MQ=255)
                                                                                                                  aCTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAggg                              <  2:431606/151‑1 (MQ=255)
                                                                                                                         ccccccGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCg                        >  2:62183/1‑150 (MQ=255)
                                                                                                                         ccccccGGGCTTGCCAGAAACCTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGc                       <  2:138025/151‑1 (MQ=255)
                                                                                                                                   ttGCCAGGAATCTTACCCCCGGTTTGGCCCTTTTGACTACCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTa             <  1:392229/151‑1 (MQ=255)
                                                                                                                                          aaacctaaaCCCCGGTTTGGCCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGc       <  2:318551/143‑1 (MQ=255)
                                                                                                                                          aaaaCTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGc       <  2:385325/149‑1 (MQ=255)
                                                                                                                                              cTTAACCCCGGTTTGGCCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATcc  <  2:730195/151‑1 (MQ=255)
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TGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGTGCCCCCCGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCC  >  NC_000913/3177383‑3177675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑