Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,060,146	C→A	Q132K (CAG→AAG)	argP →	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,060,146	0	C	A	100.0%	144.7 / NA	39	Q132K (CAG→AAG)	argP	transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/26);  total (13/26)

ATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCCGAAAAATAT  >  NC_000913/3060000‑3060280                                                                                                                                                   |                                                                                                                                       aTGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAgg                                                                                                                                     <  1:21612/150‑1 (MQ=255) aTGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAgg                                                                                                                                     <  2:309092/150‑1 (MQ=255) aTGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAgg                                                                                                                                     <  1:421333/150‑1 (MQ=255)    aaCAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCCCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACg                                                                                                                                 >  1:170883/1‑151 (MQ=255)           cGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTgcgc                                                                                                                          <  1:260276/151‑1 (MQ=255)           cGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTgcgc                                                                                                                          <  2:108493/151‑1 (MQ=255)                     ccGCTGCTGCTTTCACTGGCGGTCAACGCCCACAGTCTGGCGCCGGGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCCATCCGCCTCAACTTGCAGGTAGAAGCTGAAACCCGCACTAACGAACGTCTGCGCCGCGGCGAAg                                                                                                                >  1:643668/1‑151 (MQ=255)                        ctgctgctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTgg                                                                                                             <  2:697353/151‑1 (MQ=255)                           ctgctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTc                                                                                                           <  2:706210/150‑1 (MQ=255)                           ctgctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACATGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTc                                                                                                           <  1:208981/150‑1 (MQ=255)                            tgctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGGAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGGGGTCgg                                                                                                         >  1:680505/1‑151 (MQ=255)                             gctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCgg                                                                                                         >  2:572804/1‑150 (MQ=255)                             gctTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCgg                                                                                                         >  2:547433/1‑150 (MQ=255)                                           tCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTc                                                                                           <  2:637715/150‑1 (MQ=255)                                           tCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTc                                                                                           <  1:582287/150‑1 (MQ=255)                                           tCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTc                                                                                           <  2:350585/150‑1 (MQ=255)                                             aaCGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAAc                                                                                        <  1:593319/151‑1 (MQ=255)                                               cGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACAt                                                                                      <  1:589057/151‑1 (MQ=255)                                                      aGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGAGGTGAGTATTCAACATCAGgcg                                                                                >  1:370282/1‑150 (MQ=255)                                                                  tGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGttgt                                                                    >  2:293153/1‑150 (MQ=255)                                                                   ggTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGttgt                                                                    >  2:146190/1‑149 (MQ=255)                                                                         ttCCTGCACTGTCGCCTGTGTTGGCTGTTTCGCCTATCCCCCTCAACTTGCAGGCAGAAGCTGATACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGa                                                            >  1:308383/1‑151 (MQ=255)                                                                         ttCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGa                                                            >  1:25444/1‑151 (MQ=255)                                                                              gCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATaaa                                                        >  1:454937/1‑150 (MQ=255)                                                                               cACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACt                                                      <  1:187571/151‑1 (MQ=255)                                                                                  tGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTgg                                                   <  2:393/151‑1 (MQ=255)                                                                                  tGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTgg                                                   <  2:617093/151‑1 (MQ=255)                                                                                     cTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTg                                                 <  2:457509/150‑1 (MQ=255)                                                                                     cTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTg                                                 <  1:567441/150‑1 (MQ=255)                                                                                     cTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTg                                                 <  1:407226/150‑1 (MQ=255)                                                                                     cTCATGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTg                                                 >  1:482225/1‑150 (MQ=255)                                                                                          gtgtTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCg                                           >  1:87654/1‑151 (MQ=255)                                                                                             ttGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGAc                                         <  1:715205/150‑1 (MQ=255)                                                                                                 cTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATc                                     <  2:130784/150‑1 (MQ=255)                                                                                                 cTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCt                                    <  2:100889/151‑1 (MQ=255)                                                                                                 cTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCt                                    <  1:242172/151‑1 (MQ=255)                                                                                                 cTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCt                                    <  2:711842/151‑1 (MQ=255)                                                                                                         ccTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTc                             <  2:725023/150‑1 (MQ=255)                                                                                                                                     aTGAAACCCGCACTAAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCCGAAAAatat  <  1:33232/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                       ATGAACAAACCGGTTCGACTCCGCTGCTGCTTTCACTGGCGGTCAACGCCGACAGTCTGGCGACGTGGTTGCTTCCTGCACTGGCTCCTGTGTTGGCTGATTCGCCTATCCGCCTCAACTTGCAGGTAGAAGATGAAACCCGCACTCAGGAACGTCTGCGCCGCGGCGAAGTGGTCGGCGCGGTGAGTATTCAACATCAGGCGCTGCCGAGTTGTCTTGTCGATAAACTTGGTGCGCTCGACTATCTGTTCGTCAGCTCAAAACCCTTTGCCGAAAAATAT  >  NC_000913/3060000‑3060280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑