Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,471,274 C→A G19V (GGC→GTC)  tufA ← translation elongation factor EF‑Tu 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,471,2740CA98.7% 281.4 / NA 75G19V (GGC→GTC) tufAtranslation elongation factor EF‑Tu 1
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (31/43);  total (32/43)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAT  >  NC_000913/3471126‑3471420
                                                                                                                                                    |                                                                                                                                                  
aaCGTGAAATGTGTTGATGGGAAAACCCGGACCTTTTTCTTCCGGCGCGTTTTGGATCTGTGCGATTGCTCGAGCAGCAGCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACg                                                                                                                                                  <  1:139550/151‑1 (MQ=14)
      agaAGTTTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGt                                                                                                                                             <  1:313849/150‑1 (MQ=255)
        aaGTGTTGATGGTGATCCCAGGAGCTTTTTCTTCCGGCGCGTTATCGTTATGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTAcg                                                                                                                                          <  1:219952/151‑2 (MQ=255)
        aaGTGTTGATGGTGATCCCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTAc                                                                                                                                           <  1:731266/150‑1 (MQ=255)
        aaGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTAc                                                                                                                                           <  2:438558/150‑1 (MQ=255)
          gtgtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCaa                                                                                                                                        >  1:474168/1‑151 (MQ=255)
          gtgtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCaa                                                                                                                                        >  1:381905/1‑151 (MQ=255)
          gtgtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCaa                                                                                                                                        >  1:179108/1‑151 (MQ=255)
            gtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACg                                                                                                                                      >  1:584106/1‑151 (MQ=255)
            gtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACg                                                                                                                                      >  2:383104/1‑151 (MQ=255)
            gtTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACg                                                                                                                                      >  1:699534/1‑151 (MQ=255)
             ttGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACg                                                                                                                                      <  2:502645/150‑1 (MQ=255)
               gATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTa                                                                                                                                   >  2:439224/1‑151 (MQ=255)
                 tGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTaa                                                                                                                                  >  2:55941/1‑150 (MQ=255)
                 tGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTaa                                                                                                                                  >  2:4560/1‑150 (MQ=255)
                  ggTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTATGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACg                                                                                                                                >  1:530309/1‑151 (MQ=255)
                   ttGATACCCCGAGCTTTTTCTCCCGGCGCTTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACg                                                                                                                                <  1:373992/149‑1 (MQ=255)
                   gTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACg                                                                                                                                <  1:369817/150‑1 (MQ=255)
                             gAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGCCAACGTGGCCGATAGTACCAAAGTTAACGTGCGGTTTTGt                                                                                                                     >  1:561054/1‑151 (MQ=255)
                              aGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTa                                                                                                                    >  2:629044/1‑151 (MQ=255)
                                cTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTAc                                                                                                                   <  1:599004/150‑1 (MQ=255)
                                cTTTTTCTCCCGGCGCGTTCTCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTa                                                                                                                    <  1:384748/149‑1 (MQ=255)
                                       ttcCGGCGCGTTATCGATCTGGTCGAATGCACGGGCAGAACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAAt                                                                                                           <  1:452505/151‑1 (MQ=255)
                                                gTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTa                                                                                                  >  1:270624/1‑151 (MQ=255)
                                                 ttATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAg                                                                                                 >  1:88177/1‑151 (MQ=255)
                                                   aTCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGac                                                                                               <  1:350784/151‑1 (MQ=255)
                                                      gATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACg                                                                                            >  1:58863/1‑151 (MQ=255)
                                                       aTCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACgg                                                                                           >  1:714822/1‑151 (MQ=255)
                                                            gTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCtata                                                                                      >  1:286410/1‑151 (MQ=255)
                                                             tCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTatat                                                                                     <  1:553453/151‑1 (MQ=255)
                                                             tCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTatat                                                                                     <  2:670896/151‑1 (MQ=255)
                                                                aaTGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTc                                                                                   <  2:44049/150‑1 (MQ=255)
                                                                aaTGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTc                                                                                   <  2:663878/150‑1 (MQ=255)
                                                                    cACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTAc                                                                              <  1:478851/151‑1 (MQ=255)
                                                                    cACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTAc                                                                              <  2:257896/151‑1 (MQ=255)
                                                                     aCGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACt                                                                             <  1:4560/151‑1 (MQ=255)
                                                                       gAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtat                                                                           >  1:397092/1‑151 (MQ=255)
                                                                        agcagcACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtata                                                                          >  1:621268/1‑151 (MQ=255)
                                                                        agcagcACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtat                                                                           <  2:283256/150‑1 (MQ=255)
                                                                        agcagcACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtat                                                                           <  2:727617/150‑1 (MQ=255)
                                                                        agcagcACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtat                                                                           <  1:26293/150‑1 (MQ=255)
                                                                        agcagcACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACtat                                                                           <  1:123270/150‑1 (MQ=255)
                                                                          cagcaCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAg                                                                         <  1:436110/150‑1 (MQ=255)
                                                                             ccccgccgTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGGTTTGTACGTTCAAATTTTTCTTTAGACCCGGCTATATTCCTGACTATAGTGc                                                                      <  1:326286/148‑1 (MQ=255)
                                                                             caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTg                                                                       <  2:545020/149‑1 (MQ=255)
                                                                             caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  2:485937/150‑1 (MQ=255)
                                                                             caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  2:15730/150‑1 (MQ=255)
                                                                             caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  2:74433/150‑1 (MQ=255)
                                                                             caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  2:623208/150‑1 (MQ=255)
                                                                             caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  2:251116/150‑1 (MQ=255)
                                                                             caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  1:626141/150‑1 (MQ=255)
                                                                             caCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGc                                                                      <  2:715622/150‑1 (MQ=255)
                                                                              aCCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGctc                                                                    <  1:373112/151‑1 (MQ=255)
                                                                                  ccgTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTcc                                                                 >  2:227233/1‑150 (MQ=255)
                                                                                     tAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCt                                                              <  2:259869/150‑1 (MQ=255)
                                                                                       ggTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCa                                                           >  2:187009/1‑151 (MQ=255)
                                                                                        gTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTc                                                            <  1:253026/149‑1 (MQ=255)
                                                                                        gTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTc                                                            <  2:184713/149‑1 (MQ=255)
                                                                                         ttttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                         <  2:669376/151‑1 (MQ=255)
                                                                                         ttttAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAgg                                                         <  2:65280/151‑1 (MQ=255)
                                                                                             aGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                     >  1:201641/1‑151 (MQ=255)
                                                                                               ccAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGgagag                                                     <  1:579365/149‑1 (MQ=255)
                                                                                                  gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                 >  2:534430/1‑150 (MQ=255)
                                                                                                  gTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCa                                                 >  1:257403/1‑150 (MQ=255)
                                                                                                                      gTCAGAGTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAAccc                             <  2:663710/150‑1 (MQ=255)
                                                                                                                          gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  1:601083/1‑151 (MQ=255)
                                                                                                                          gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  1:631752/1‑151 (MQ=255)
                                                                                                                          gagTAGTTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  2:76767/1‑151 (MQ=255)
                                                                                                                            gtagtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAgg                        >  1:651584/1‑149 (MQ=255)
                                                                                                                             tagtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGc                       <  1:519904/149‑1 (MQ=255)
                                                                                                                             tagtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCtt                     >  2:497397/1‑151 (MQ=255)
                                                                                                                               gtTTTACCGTGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTAt                   >  2:182784/1‑151 (MQ=255)
                                                                                                                                        tGGTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACgg          >  2:574356/1‑151 (MQ=255)
                                                                                                                                         ggTCAACGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACggg         >  2:66817/1‑151 (MQ=255)
                                                                                                                                              aCGTGGACGATAGTACCAACGTTAACGTGCGGTTTTGTGCGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTG‑‑CTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAt  <  2:396147/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACGTGAGAAGTGTTGATGGTGATACCACGAGCTTTTTCTTCCGGCGCGTTATCGATCTGGTCGAATGCACGAGCAGCACCGCCGTAGGTTTTAGCCAGTACGGTGGTGATTGCAGCGGTCAGAGTAGTTTTACCGTGGTCAACGTGGCCGATAGTACCAACGTTAACGTGCGGTTTTGTACGTTCAAATTTTTCTTTAGACACGGCTATATTCCTTACTATAGTGCTCTCCCCTTCAGGAGAGAGCACGGGACTTTGGTATTAACCCTTAGGCTTATTTACCACGGGCTTCAAT  >  NC_000913/3471126‑3471420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: