Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,875,5210TC21.5% 109.7 / 21.0 65A28A (GCT→GCCyidXputative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (23/28);  new base C (14/0);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.23e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

ACCAGCGTGATGACGTTCGCGTTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAA  >  NC_000913/3875233‑3875808
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                
tccaaagtgtggaCTTTCGATTTTGCCGTACTTGTGATGTAGTACATTCTTATGTTTTTGTTCTACAATTTAGTTAACAAAAAGAAAAATGCATAAGAAAATAACAAGAACAAGGCAGAAATCGAAAACGTGATAGCAGGAGCTAAACAAGAGGGGAAATAGGGTATGCATTCAACGGCTTAGCTAATCATTTAAAGGATTAATCATGAAGTTGAATTTTAAATGATTTTTTAAGGCTGCTTGTTTGTGCCCTCTGACGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCAt                                                                                                                                                                                                                                                                                      <  2:730434/290‑1 (MQ=255)
                 tgcatatcgcgtgcttgTAGTGTAGTACAAGCTATTATTTTTGTATTACAATTTAGTGCAAAATAATAACAATGTATAAGAAAATAACATAATTTTGACCAAAAACTGAAAAAGGAAATAAGGCGCTAAACAGGAGGGAAAGAAGAGTAAGATTTCAACGGCTTAGCTACTCGGTTAAAAGATTAATCATGATGTTGAATTTTAAGGGATTTTTTAAGGCGGCCTATTTATTCCCTCTGGTGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTc                                                                                                                                                                                                                                                                     <  2:56492/292‑1 (MQ=255)
                      ttGACGTGAGTGTAATGTAGTACAAACTAATATAGTTGGACTACAATTTAGATGAAAAAAAGAACAATGAAGAAAAAAATGAAATGCGTCGGGAAGAAATCTGAAAAGGGATATCAAGAGATAAACAGGAGGGAAAGAAGAGTATGCTTTAAACGGATTAGAGACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGACAGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATATCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGaa                                                                                                                                                                                                                                                                <  2:375238/301‑1 (MQ=255)
                                              aaCTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGAcccc                                                                                                                                                                                                                                         >  2:152006/1‑300 (MQ=255)
                                              aaCTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCa                                                                                                                                                                                                                                        >  2:847138/1‑301 (MQ=255)
                                                cTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTGGTTTGCTTGAACAAAAAGGGTAGTTCAGGAAAGTATCAATCGTAGTCAAACACCAt                                                                                                                                                                                                                                       >  2:330347/1‑300 (MQ=255)
                                                cTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTGGTTTCCCATACGGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGCCACCAt                                                                                                                                                                                                                                       >  2:553575/1‑300 (MQ=255)
                                                cTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGTTTTCCTTTACATAAAAAGGTGGTTCAGGAACTTATCAATctatttacaaaacaata                                                                                                                                                                                                                                      >  2:875504/1‑285 (MQ=255)
                                                cTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTGATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTGCTATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTAAGACAccgt                                                                                                                                                                                                                                       >  2:729903/1‑298 (MQ=255)
                                                  tataTTGGTGTACTACAATTAAGGTCACAATAAGAAACATGGATAATAAAATAACAGGCGTCGGGCAGAAATCTGAAAATGAATATCAAGGGATAAACAAAAGGGAAAGAAAAGTATTCATTCAACGTCTTAGCTACTCGTTTAAAGGATTAATCATGATGTTGAATTTTAAGGGATTTTTTAAAGCGGCCGGTTGATACCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCAc                                                                                                                                                                                                                                    <  2:653081/301‑1 (MQ=255)
                                                      ttgttgTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTCTTTTGCTTTACAGCAAAGATTACGTCTGGAAAGAATTTATCCCAGTCAGAACCCAttattgcg                                                                                                                                                                                                                                >  2:666175/1‑294 (MQ=255)
                                                        gttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTggg                                                                                                                                                                                                                                >  2:746479/1‑299 (MQ=255)
                                                        gttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTCTCCTTTACCACAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTa                                                                                                                                                                                                                             >  2:657603/1‑301 (MQ=255)
                                                                        aaacaaaaaaaGCAAAATGCATAAAAAAATGACATGAGGCGGGCAGAAATCTGAAAAGGTATATCAGGCGATAAAAATGAGGTAAAGAAGAATATGCTTTCAAAAAATTAGCTAGTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGAATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGAt                                                                                                                                                                                                              <  2:506879/295‑1 (MQ=255)
                                                                         aaaaaaaaaaGAACAATGCAGAACAAGAGGACAGGCGTCGGGGTGAAATCTGAAAAGGAAGATCAGGAGATAAACATGAGGGAAAGAAGATTATGCTTTCAAAAACATAGCTACTAGTTAAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGACGGTTTATTCCAACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGa                                                                                                                                                                                                               <  2:267264/294‑1 (MQ=255)
                                                                             caAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGtn                                                                                                                                                                                                                                                                                            >  1:628213/1‑217 (MQ=255)
                                                                             caAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTc                                                                                                                                                                                                                                                                     >  2:138540/1‑241 (MQ=255)
                                                                             caAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTaa                                                                                                                                                                                                         >  2:122578/1‑301 (MQ=255)
                                                                                    aaCAATGCATAAAAAAATGACAAGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCAt                                                                                                                                                                                                                                                                                      <  1:243547/217‑1 (MQ=255)
                                                                                                    aTGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTc                                                                                                                                                                                                                                                                     <  1:138539/218‑1 (MQ=255)
                                                                                                    aTGACATGAGTCGGGCAGAAAACTGAAAAAGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTc                                                                                                                                                                                                                                                                     <  1:179093/218‑1 (MQ=255)
                                                                                                       aCATGAGGCGGGCAGAAAGCTGAAACGGGATGTCAGGCGCTAAACAGGAGGGAAAGAAGATTATGCTTTAAACTGCTTAGCTACTCGTTTAAAGGATTAATCAAGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTa                                                                                                                                                                                <  2:697588/300‑1 (MQ=255)
                                                                                                                gggCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCACAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTTGGCTATCGCAGGCAAACGAt                                                                                                                                                                                                              >  2:513200/1‑261 (MQ=255)
                                                                                                                gggCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCg                                                                                                                                                                       >  2:516113/1‑300 (MQ=255)
                                                                                                                       aaTCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCCTTACCAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATGACTTGGCTATCCCAGGTCACAGATAGTAACTGAAATAAAGTCTAGGGTTTTGTTGTGGAATTGCTGGAttt                                                                                                                                                               >  2:828228/1‑300 (MQ=255)
                                                                                                                         tCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAACAAAAAAGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATTGAACAAAAGGCTATGTCTTAGTATGGAACTCGCTGGATTAc                                                                                                                                                              >  2:521952/1‑300 (MQ=255)
                                                                                                                                 gggATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCa                                                                                                                                                      >  2:619203/1‑299 (MQ=255)
                                                                                                                                 gggATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCCTTACAGAAAAGGGTAGTTCAGGAAATTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCCGTCCAAAGATTGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTTTCa                                                                                                                                                      >  2:416938/1‑299 (MQ=255)
                                                                                                                                  ggATATCAGGAGCGAAACAGGAGGGAAAGAAGATTATGCTTCCAACGGCTTAGGTAATCGTTTAAAGGATTAATCATGAAGTTGAATGTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTTGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAc                                                                                                                                                     <  2:311337/299‑1 (MQ=255)
                                                                                                                                     natCAAGCACTAAACAGGAGGTAAAGAAGAGTATGCTGTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCAc                                                                                                                                                                                                                                    <  1:807393/217‑1 (MQ=255)
                                                                                                                                      atCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCTTGACCGAAAAAGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAAAAAACGGCTATGTTTTTGTAGGGGAAACGCTGAATTACCTTCTCACTGATgg                                                                                                                                               >  2:638571/1‑301 (MQ=255)
                                                                                                                                                aaaCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCATGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGaa                                                                                                                                     >  2:700142/1‑300 (MQ=255)
                                                                                                                                                     ggaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTAAGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCa                                                                                                                                                                                                                    >  1:532540/1‑218 (MQ=255)
                                                                                                                                                        gggAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTAGTTCAGGAATGTATCAATCGCCGTCAGACTACATCACTGGGCTCTCGCAGGCAAAAGATATTAATGGAACAAAAGGCTATGTTTTTGTAGTGGAATCGCTGGATTACCTTATACACTATGGATCCGATGAACTCGTTaa                                                                                                                             >  2:453194/1‑301 (MQ=255)
                                                                                                                                                         ggAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCaaaan                                                                                                                                                                                                                >  1:646982/1‑217 (MQ=255)
                                                                                                                                                         ggAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTAAGGCTGCCGGTTTATTCCCACCGGCGCTGATGCCTTTAGGCTGTATCTCGTATGCCCTGGTTTCCCTTACCGAAAAGGGTAGTTTAGGAAAGTATCAATCGCAGTTCGAAACCATCACCGGGCTATCCCAGGCCAAAGATAGTAATGGAACAAAAGGGTATGGTTTTGTAGGGGAATTGCCGGGTTACCGTACTACTGAATGTGCCGGTGAAATCGGTTAGa                                                                                                                           >  2:330766/1‑301 (MQ=255)
                                                                                                                                                          gAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGTTCTGCTTTAAAGAAAAGGGTAGTTCAGGAAAGTATCAATCGCCGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTCATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCTCTGGATGTCCGTATCACTGATGGTGCCGATAACATCGTTAAGa                                                                                                                           >  2:656438/1‑301 (MQ=255)
                                                                                                                                                          aaatgaagaGTATGAGTTCAAAGGCTAAGCAACTGGTTTAAAGGATTAATCAGGAAGTTGAATATGAAAGGATTTTTTAAGTCTGCCGGTTAATTCCAACAGACGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGa                                                                                                                           <  2:226096/297‑1 (MQ=255)
                                                                                                                                                           aaaGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCAGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGAt                                                                                                                                                                                                              <  1:513195/218‑1 (MQ=255)
                                                                                                                                                              naagaGTATGCTTGCAAAGGCTTATCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGAGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGt                                                                                                                                                                                                           <  1:712528/217‑1 (MQ=255)
                                                                                                                                                              gaagaGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGGTTTGCCAGAAAAAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATg                                                                                                                         >  2:688840/1‑299 (MQ=255)
                                                                                                                                                                 nataaTGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAAt                                                                                                                                                                                                        <  1:847111/214‑1 (MQ=255)
                                                                                                                                                                  agTATGCTTTAAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTaa                                                                                                                                                                                                         <  1:746458/216‑1 (MQ=255)
                                                                                                                                                                    taagCTTTCAAAGGCTTGGCTACTCGTTTAAAGAATTAATCATTAAGTTGAATTTTAAAGGCTTTTTTAAGGCTGCCGGTTTAGTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGATTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGGAGTCAGACACCATCACTGGGGTATCGCAGGCAAAAGATAGTAATGAAACAAAAGGCTATGGTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGa                                                                                                                 <  2:646993/298‑1 (MQ=255)
                                                                                                                                                                                       cTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGTAGTTCCGGAAAGTATCAATTGCAGTCAGACATCATCAAGGGGCTATCGAACGCAAAAGATACTAAGGGAATCAAAGGCTTTGTTTTTGTAGGGGAAATGTTGGATTCCCTTATCCGAGATGGTTCGGATGACATAGTTTAGATGCTCATTGATCAAGCAATTAAcgtgaa                                                                                               >  2:367060/1‑295 (MQ=255)
                                                                                                                                                                                        tACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATGTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGt                                                                                                                                                                                 <  1:86146/218‑1 (MQ=255)
                                                                                                                                                                                          atCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGAAAAGGGTAGTTCAGGAAAGTATAAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAat                                                                                           <  2:252119/300‑1 (MQ=255)
                                                                                                                                                                                            cGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAACAAAAAGAATAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCACACGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTTGCTTACCTTATAACTACTGGTTCCGATTACATCGTTAAGATGCTAAATGATCCAGCACTTAACCGGCAAAttat                                                                                         >  2:435933/1‑301 (MQ=255)
                                                                                                                                                                                            cGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGAGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGGTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGCTGCTCATTGCTCCAGCACTTAACCGGCACAgtat                                                                                         >  2:861679/1‑301 (MQ=255)
                                                                                                                                                                                             gTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATTGCTGGATTACCTTATCACTGATGGTTCCGATGACATCGTTAAGATGGTCAATGAACCAGAACTAAACCGGCACAAtttt                                                                                        >  2:535721/1‑301 (MQ=255)
                                                                                                                                                                                                    ggATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTCTCCCTGACCGAAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAACAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCAAGCCCTTACACGGcccactctcaatcttg                                                                                 >  2:837281/1‑289 (MQ=255)
                                                                                                                                                                                                                  gTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTTGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGCCGCAAGa                                                                   >  2:152333/1‑301 (MQ=255)
                                                                                                                                                                                                                   ntGAATTTTAAGGGATTTTTTAAGGCTGCCGATTTAATCCCACGGGCGCTGATGCTTTCAGTCTGTATCTCGTATGCTCTGGTTGCCCATACCGCAAAGGGTAGTTCAGTAAAGTATCAATCGCAGTCAGACTCCATCACTGGGCTATCGCAGGCAAAAGATAGTAATTGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAc                                                                                                                                                     <  1:729884/217‑1 (MQ=255)
                                                                                                                                                                                                                   ntGAATTTTAAGGGATTATTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCAc                                                                                                                                                     <  1:820656/217‑1 (MQ=255)
                                                                                                                                                                                                                              tggATTTGTTAAGTCTGGCGGTTTATGCCGACTGGGGGTGATGCTTCCAGGCTGTATCTCTTATGCTATGGTTTCCCATACCTAAAAGGGTAGTTAAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGACAAAAGATAATAATGGAACAAAAGGATATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGGGCCGATGACATCGTTAAGATGCTAAATGATCCAGCACTTAACCGGCACAATATTCTGGTTGCCGATGACGCAAGATTTGTTTTAAAt                                                       <  2:752922/300‑1 (MQ=255)
                                                                                                                                                                                                                                 nttttttAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATg                                                                                                                                       <  1:619192/217‑1 (MQ=255)
                                                                                                                                                                                                                                   tttttAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTTGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGAc                                                                                                                                     <  1:516107/218‑1 (MQ=255)
                                                                                                                                                                                                                                    ttttAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCCCAATGATCCAGCACTTAACCGGCACAATATTCAgg                                                                                    >  2:725087/1‑266 (MQ=255)
                                                                                                                                                                                                                                             tGCCGGTTTATTCCCACTGGCGCTGATGCTTTAAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGa                                                                                                                           <  1:330764/218‑1 (MQ=255)
                                                                                                                                                                                                                                                 ggTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTACGATg                                                                                                                         >  1:261701/1‑216 (MQ=255)
                                                                                                                                                                                                                                                 ggTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGGAGGGGAATCGCTGGATTAACTTATCACTGATGGTGCCGATGACATCGTTAAGATg                                                                                                                         >  1:800690/1‑216 (MQ=255)
                                                                                                                                                                                                                                                                 cgcTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACtt                                                                                                       >  1:335122/1‑218 (MQ=255)
                                                                                                                                                                                                                                                                                 atGTATTGCGGATGCTCTGGTTTCCCATACCGCAAAGGGTAGTGCAGGAAAGTATAAATCGCAGTCAGAAAACATCACGGGGGTATCGCAGGAAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAAGAGCTGGATTAACTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGAACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTGCTACTGGaa     <  2:628224/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   gTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATaa  >  2:70674/1‑301 (MQ=255)
                                                                                                                                                                                                                                                                                    naTCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGTCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAgg                                                                                    <  1:725069/217‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               ctctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGCTGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGa                                                                          >  1:259080/1‑217 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                
ACCAGCGTGATGACGTTCGCGTTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGGAATAA  >  NC_000913/3875233‑3875808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: