BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,187,352	T→A	E237D (GAA→GAT)	rsbU ←	Phosphoserine phosphatase RsbU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,187,352	0	T	A	100.0%	1310.5 / NA	333	E237D (GAA→GAT)	rsbU	Phosphoserine phosphatase RsbU
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (178/155); total (178/155)

Only 100 of 349 total aligned reads displayed.

TAATTAAATCATCAAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATTTCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGTTCATTTCTTC  >  USA300TCH1516_ALE/2187264‑2187438

tAATTAAATCATCAAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATatc                                                                                      >  1:3273808/1‑91 (MQ=255)

 aaTTAAATCATCAAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATatct                                                                                     >  2:2767416/1‑91 (MQ=255)

    tAAATCATCAAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCa                                                                                  <  1:172433/91‑1 (MQ=255)

       atcatcAAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAt                                                                               >  2:4288319/1‑91 (MQ=255)

        tcatcaAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAtt                                                                              <  2:5427877/91‑1 (MQ=255)

        tcatcaAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAtt                                                                              <  1:1793558/91‑1 (MQ=255)

         catcaAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAttc                                                                             >  1:3346667/1‑91 (MQ=255)

          atcaAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAttct                                                                            >  2:1536433/1‑91 (MQ=255)

          atcaAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAttct                                                                            >  2:1475494/1‑91 (MQ=255)

          atcaAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAttct                                                                            >  1:1638382/1‑91 (MQ=255)

           tcaAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAttctt                                                                           >  1:6504500/1‑91 (MQ=255)

            caAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAttcttc                                                                          >  2:4626118/1‑91 (MQ=255)

            caAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAAttcttc                                                                          >  1:3505456/1‑91 (MQ=255)

             aaGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATtcttct                                                                         >  2:2158119/1‑91 (MQ=255)

                gTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCtttt                                                                      <  2:4105058/91‑1 (MQ=255)

                 tataTTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCttttt                                                                     <  2:662749/91‑1 (MQ=255)

                    atTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAg                                                                  >  2:642893/1‑91 (MQ=255)

                    atTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAg                                                                  >  2:554945/1‑91 (MQ=255)

                     ttGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAgc                                                                 <  2:104440/91‑1 (MQ=255)

                     ttGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAgc                                                                 <  1:3118778/91‑1 (MQ=255)

                     ttGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAgc                                                                 >  2:4857389/1‑91 (MQ=255)

                     ttGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAgc                                                                 >  1:1963522/1‑91 (MQ=255)

                     ttGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAgc                                                                 <  1:5624605/91‑1 (MQ=255)

                      tGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAgcg                                                                <  1:2880685/91‑1 (MQ=255)

                        gAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGcgcg                                                              >  2:4000168/1‑91 (MQ=255)

                         aaTTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGa                                                             >  1:5728518/1‑91 (MQ=255)

                         aaTTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGa                                                             >  1:3750910/1‑91 (MQ=255)

                          aTTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGAt                                                            >  2:2320167/1‑91 (MQ=255)

                          aTTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGAt                                                            >  1:3061207/1‑91 (MQ=255)

                            ttCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATaa                                                          <  1:4282353/91‑1 (MQ=255)

                             tCTTGTTGTTGATATCGTGTTTGTGGACTGATTCATAACACTCTACCCCTAACTGACATATCTTCAAATTCTTCTTTTTCAGCGCGCTaaa                                                         >  2:6268456/1‑91 (MQ=255)

                              cTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAat                                                        >  1:4689410/1‑91 (MQ=255)

                              cTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAat                                                        <  1:4977385/91‑1 (MQ=255)

                              cTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAat                                                        >  1:662772/1‑91 (MQ=255)

                                 gttgttgATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAatata                                                     >  2:4701097/1‑91 (MQ=255)

                                 gttgttgATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAatata                                                     <  1:2407646/91‑1 (MQ=255)

                                 gttgttgATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAatata                                                     <  1:5381675/91‑1 (MQ=255)

                                   tgttgATATCGTGTTTGTGAACTGATTCCTAGCACTCTACCTCCGCCTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATc                                                   <  2:2707490/91‑1 (MQ=255)

                                   tgttgATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATc                                                   >  2:6538537/1‑91 (MQ=255)

                                   tgttgATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATc                                                   <  2:2451200/91‑1 (MQ=255)

                                      tgATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAg                                                <  1:4593062/91‑1 (MQ=255)

                                       gATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAgg                                               >  1:1474526/1‑91 (MQ=255)

                                        atatCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGc                                              <  2:4772537/91‑1 (MQ=255)

                                            cGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCAt                                          >  1:1671432/1‑91 (MQ=255)

                                            cGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCAt                                          >  1:1019475/1‑91 (MQ=255)

                                             gtgtTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATg                                         >  1:2372198/1‑91 (MQ=255)

                                             gtgtTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATg                                         >  1:2587482/1‑91 (MQ=255)

                                             gtgtTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATg                                         >  2:1357742/1‑91 (MQ=255)

                                              tgtTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGa                                        <  2:3106419/91‑1 (MQ=255)

                                              tgtTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGa                                        >  1:5895981/1‑91 (MQ=255)

                                               gtTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCGTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGAc                                       <  2:5053901/91‑1 (MQ=255)

                                                 ttGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCa                                     >  1:5303281/1‑91 (MQ=255)

                                                 ttGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCa                                     <  2:449644/91‑1 (MQ=255)

                                                 ttGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCa                                     <  2:5895981/91‑1 (MQ=255)

                                                  tgtgAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAg                                    >  1:1325207/1‑91 (MQ=255)

                                                  tgtgAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAg                                    <  1:2698272/91‑1 (MQ=255)

                                                  tgtgAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAg                                    <  2:3507918/91‑1 (MQ=255)

                                                  tgtgAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAg                                    <  1:3838610/91‑1 (MQ=255)

                                                   gtgAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGc                                   >  1:639324/1‑91 (MQ=255)

                                                     gAACTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTg                                 >  1:5135338/1‑91 (MQ=255)

                                                      aaCTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGa                                <  1:6278374/91‑1 (MQ=255)

                                                       aCTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTgca                               >  1:1071615/1‑89 (MQ=255)

                                                       aCTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGaa                               >  2:1219250/1‑91 (MQ=255)

                                                        cTGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAAc                              >  2:2265369/1‑91 (MQ=255)

                                                         tGATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACt                             <  2:3897493/91‑1 (MQ=255)

                                                          gATTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACta                            <  2:3584920/91‑1 (MQ=255)

                                                           aTTCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtac                           <  2:2547051/91‑1 (MQ=255)

                                                            ttCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtaca                          >  1:5278157/1‑91 (MQ=255)

                                                             tCCTAACCCTCTGCCTCTAACTGAAATATCTTCAAATTCTTCGTTTTCAGCGCGATAAATATATCCAGTTTCATGACCAGCTGATCtacaa                         <  2:4614792/91‑1 (MQ=255)

                                                             tCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtacaa                         <  2:1874398/91‑1 (MQ=255)

                                                             tCCTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtacaa                         >  2:3372393/1‑91 (MQ=255)

                                                             tCCTAACACACTACCTTTAACTGAAATATATTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtacaa                         >  1:6040192/1‑91 (MQ=255)

                                                              ccTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtacaat                        >  1:725361/1‑91 (MQ=255)

                                                              ccTAACACTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtacaat                        >  1:5195678/1‑91 (MQ=255)

                                                                  acacTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtacaatacaa                    >  1:4520532/1‑91 (MQ=255)

                                                                  acacTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtacaatacaa                    >  2:574120/1‑91 (MQ=255)

                                                                  acacTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACtacaatacaa                    <  2:517327/91‑1 (MQ=255)

                                                                    acTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTAcaatacaata                  >  2:77797/1‑91 (MQ=255)

                                                                    acTCTACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAACATATCCAGGCTCATGACCAGCTGAACTAcaatacaata                  >  1:5463494/1‑91 (MQ=255)

                                                                      tctACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATaaa                >  1:978045/1‑91 (MQ=255)

                                                                      tctACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATaaa                >  2:1695140/1‑91 (MQ=255)

                                                                      tctACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATaaa                >  1:89286/1‑91 (MQ=255)

                                                                        tACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATg              <  2:2874611/91‑1 (MQ=255)

                                                                        tACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATg              >  2:3710920/1‑91 (MQ=255)

                                                                        tACCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATg              >  2:1284838/1‑91 (MQ=255)

                                                                         aCCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATgg             >  1:3469814/1‑91 (MQ=255)

                                                                         aCCTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATgg             >  2:1675733/1‑91 (MQ=255)

                                                                          ccTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGt            <  2:821974/91‑1 (MQ=255)

                                                                          ccTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGt            >  1:3704840/1‑91 (MQ=255)

                                                                          ccTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGt            >  1:4055174/1‑91 (MQ=255)

                                                                          ccTCTAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGt            >  1:788635/1‑91 (MQ=255)

                                                                           ctctAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGtt           <  2:1963066/91‑1 (MQ=255)

                                                                           ctctAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGtt           <  1:1186601/91‑1 (MQ=255)

                                                                            tctAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGTTc          >  1:5019616/1‑91 (MQ=255)

                                                                            tctAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGTTc          <  2:2093102/91‑1 (MQ=255)

                                                                              tAACTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGTTCAt        >  1:2307371/1‑91 (MQ=255)

                                                                               aaCTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGTTCAtt       >  2:5462746/1‑91 (MQ=255)

                                                                                aCTGAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGTTCAttt      >  1:2098758/1‑91 (MQ=255)

                                                                                   gAAATATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGTTCATttctt   <  2:1110974/91‑1 (MQ=255)

                                                                                    aaaTATCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTAGAATACAATAAATGGTTCATttcttc  >  2:6112136/1‑91 (MQ=255)

TAATTAAATCATCAAGGTATATTGGAATTTCTTGTTGTTGATATCGTGTTTGTGAACTGATTCCTAACACTCTACCTCTAACTGAAATTTCTTCAAATTCTTCTTTTTCAGCGCGATAAATATATCCAGGCTCATGACCAGCTGAACTACAATACAATAAATGGTTCATTTCTTC  >  USA300TCH1516_ALE/2187264‑2187438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`