BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	99.6%	1024.7 / NA	270	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (138/131); minor base T (0/1); total (138/132)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

TACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTT  >  USA300TCH1516_ALE/1646101‑1646274

tACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGcctcct                                                                                     >  1:3616600/1‑91 (MQ=255)

 aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCtt                                                                                    <  2:5563532/91‑1 (MQ=255)

 aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCtt                                                                                    >  2:1245901/1‑91 (MQ=255)

  cAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCttt                                                                                   >  1:756723/1‑91 (MQ=255)

   aaTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCtttt                                                                                  >  2:837750/1‑91 (MQ=255)

   aaTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCtttt                                                                                  <  2:1800374/91‑1 (MQ=255)

    aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTTCCTTTAATTC‑TTTTTCAGTCATACCTCCTTTTa                                                                                 >  2:5246745/1‑91 (MQ=255)

    aTCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCAGTTGTCTTTCTAATATTGCCTTTAATTC‑TTGTTCAGTCATGCCTCCTTTTa                                                                                 <  2:2132215/91‑1 (MQ=255)

     tCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTac                                                                                >  1:2947926/1‑91 (MQ=255)

      cGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTaca                                                                               <  2:100356/91‑1 (MQ=255)

      cGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTaca                                                                               <  1:3356020/91‑1 (MQ=255)

      cGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTaca                                                                               <  2:1558090/91‑1 (MQ=255)

       gTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTacaa                                                                              <  1:270394/91‑1 (MQ=255)

       gTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTacaa                                                                              >  1:6478118/1‑91 (MQ=255)

       gTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTacaa                                                                              >  1:4447915/1‑91 (MQ=255)

           cGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTTCAGTCATGCCTCCTTTTACAACAg                                                                           >  2:2994798/1‑91 (MQ=255)

                gatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                     <  2:1126166/91‑1 (MQ=255)

                 atgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAat                                                                    >  2:2288553/1‑91 (MQ=255)

                  tgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAata                                                                   >  2:5583110/1‑91 (MQ=255)

                   ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatat                                                                  >  1:5458133/1‑91 (MQ=255)

                   ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatat                                                                  >  2:3795335/1‑91 (MQ=255)

                     tCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAActatat                                                                >  2:4840730/1‑91 (MQ=255)

                      ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                               >  1:4355414/1‑91 (MQ=255)

                      ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                               <  2:3475641/91‑1 (MQ=255)

                      ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                               <  1:334351/91‑1 (MQ=255)

                      ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                               >  1:3065698/1‑91 (MQ=255)

                      ccATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                               >  2:6298964/1‑91 (MQ=255)

                       cATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTc                                                              <  1:2351087/91‑1 (MQ=255)

                         tCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAATAGTTAAAATATATTCat                                                            <  1:4984236/91‑1 (MQ=255)

                         tCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAacatatgaat                                                            <  2:756723/91‑5 (MQ=255)

                         tCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCat                                                            <  1:975952/91‑1 (MQ=255)

                          cTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                           >  2:2738339/1‑91 (MQ=255)

                          cTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCata                                                           <  1:6343708/91‑1 (MQ=255)

                          cTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATACATTCata                                                           >  1:5254896/1‑91 (MQ=255)

                            tcTCCTATTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTTCTTTTACAACAGTTAAAATATATTCATATg                                                         <  1:5236507/90‑1 (MQ=255)

                             cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGt                                                        >  2:593743/1‑91 (MQ=255)

                             cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGt                                                        <  2:1629620/91‑1 (MQ=255)

                             cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGt                                                        >  1:3538170/1‑91 (MQ=255)

                             cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTATTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATCTGt                                                        >  2:6371832/1‑91 (MQ=255)

                              tCCTAATTCTAGCATTTTTCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGtt                                                       >  2:4712614/1‑91 (MQ=255)

                               ccTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGttt                                                      >  1:166129/1‑91 (MQ=255)

                                   aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatc                                                  <  2:5891714/91‑1 (MQ=255)

                                   aTTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatc                                                  >  1:1375703/1‑91 (MQ=255)

                                     tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                <  1:3555761/91‑1 (MQ=255)

                                     tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                <  2:3615274/91‑1 (MQ=255)

                                      cTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaac                                               <  2:106640/91‑1 (MQ=255)

                                       tAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaaca                                              <  2:5341873/91‑1 (MQ=255)

                                        aGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaacaa                                             >  1:5680938/1‑91 (MQ=255)

                                         gCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAat                                            >  1:4678536/1‑91 (MQ=255)

                                         gCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAat                                            >  1:3438389/1‑91 (MQ=255)

                                         gCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAat                                            >  1:1592842/1‑91 (MQ=255)

                                           aTTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAatat                                          >  2:1891000/1‑91 (MQ=255)

                                              ttGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAg                                       >  1:4026676/1‑91 (MQ=255)

                                              atGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAg                                       <  1:3896226/90‑1 (MQ=255)

                                               tGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAACTATATTCAGATGTTTTATCAACAATATTAgc                                      >  2:6479647/1‑91 (MQ=255)

                                               tGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgc                                      <  2:1470587/91‑1 (MQ=255)

                                                gCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgct                                     <  2:5458133/91‑1 (MQ=255)

                                                gCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgct                                     <  2:2910612/91‑1 (MQ=255)

                                                 cTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                    >  2:4198747/1‑91 (MQ=255)

                                                 cTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                    <  2:5751110/91‑1 (MQ=255)

                                                 cTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                    <  1:1008378/91‑1 (MQ=255)

                                                 cTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                    >  1:5843767/1‑91 (MQ=255)

                                                 cTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                    <  1:678083/91‑1 (MQ=255)

                                                 cTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTATACAACAGTTAAAAGATATTCATATGTTTCATCAACAATAATAgctg                                    <  2:5693110/91‑1 (MQ=255)

                                                  tttCTAATATTGCCTTTAATTC‑TTTTTTTTTTTTTTTTCCTTATACAACAGTTAAAATATATTCATATGTTTCATCAATCATATTAgctgc                                   >  2:5774022/1‑91 (MQ=255)

                                                  tttCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                   >  2:2667419/1‑91 (MQ=255)

                                                  tttCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                   <  2:3259533/91‑1 (MQ=255)

                                                  tttCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                   <  1:2543627/91‑1 (MQ=255)

                                                  tttCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgc                                   >  2:4122753/1‑91 (MQ=255)

                                                   ttCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctgct                                  >  2:4230288/1‑91 (MQ=255)

                                                    tCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtt                                 <  2:1354131/91‑1 (MQ=255)

                                                     cTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTGCAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttt                                >  2:6073127/1‑91 (MQ=255)

                                                     cTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttt                                <  1:1456735/91‑1 (MQ=255)

                                                      tAATATTGCCTTTACTTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAATAATATTAGCTGCtttt                               <  1:6045385/91‑1 (MQ=255)

                                                      tAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                               <  1:5332985/91‑1 (MQ=255)

                                                      tAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                               >  2:277927/1‑91 (MQ=255)

                                                      tAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                               <  2:3556190/91‑1 (MQ=255)

                                                       aaTATTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCttttt                              <  2:5162341/91‑1 (MQ=255)

                                                        atatTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTg                             <  1:5920269/91‑1 (MQ=255)

                                                         tatTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGa                            >  1:413379/1‑91 (MQ=255)

                                                         tatTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGa                            >  1:2862598/1‑91 (MQ=255)

                                                          atTGCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGaa                           >  1:383973/1‑91 (MQ=255)

                                                             gCCTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTa                        >  1:785743/1‑91 (MQ=255)

                                                              ccTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                       <  2:4881660/91‑1 (MQ=255)

                                                              ccTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaa                       >  1:6258077/1‑91 (MQ=255)

                                                               cTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaaa                      >  2:3936810/1‑91 (MQ=255)

                                                               cTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTaaa                      >  2:6147746/1‑91 (MQ=255)

                                                               cTTTAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTAATCAACAATATTAGCTGCTTTTTGAATCaaa                      >  1:6123449/1‑91 (MQ=255)

                                                                 ttAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGc                    >  1:5491635/1‑91 (MQ=255)

                                                                  tAATTC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTACCTGCTTTTTGAATTAAAGCa                   >  2:5843512/1‑91 (MQ=255)

                                                                      tC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAttt               >  1:459410/1‑91 (MQ=255)

                                                                      tC‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAttt               <  2:1732814/91‑1 (MQ=255)

                                                                       c‑TTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTc              >  2:2444428/1‑91 (MQ=255)

                                                                         tttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCg             <  1:861933/91‑1 (MQ=255)

                                                                            ttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCa          >  2:564609/1‑91 (MQ=255)

                                                                             tCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAg         <  1:1146702/91‑1 (MQ=255)

                                                                              cAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGc        >  2:1965827/1‑91 (MQ=255)

                                                                                gTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt      <  1:3985680/91‑1 (MQ=255)

                                                                                   aTGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCtt   <  2:4003601/91‑1 (MQ=255)

                                                                                    tGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCttt  >  1:1778265/1‑91 (MQ=255)

TACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTC‑TTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTT  >  USA300TCH1516_ALE/1646101‑1646274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`