mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | PAL20_S20_L007_R1_001.good.fq | 6,600,756 | 599,382,936 | 100.0% | 90.8 bases | 91 bases | 98.9% |
| errors | PAL20_S20_L007_R2_001.good.fq | 6,600,756 | 599,382,936 | 100.0% | 90.8 bases | 91 bases | 97.9% |
| total | 13,201,512 | 1,198,765,872 | 100.0% | 90.8 bases | 91 bases | 98.4% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | USA300TCH1516_ALE | 2,872,915 | 380.8 | 5.3 | 92.9% | Staphylococcus species strain strain. |
| coverage | distribution | CP000731 | 27,041 | 1480.9 | 7.1 | 3.4% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
| coverage | distribution | NC_012417 | 3,125 | 14523.8 | 83.8 | 3.7% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
| total | 2,903,081 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 97721 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 4 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1208 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.079 |
| reference sequence | pr(no read start) |
|---|---|
| USA300TCH1516_ALE | 0.24417 |
| CP000731 | 0.03703 |
| NC_012417 | 0.02768 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 13:53:13 23 May 2019 | 13:55:53 23 May 2019 | 2 minutes 40 seconds |
| Read alignment to reference genome | 13:55:53 23 May 2019 | 14:14:22 23 May 2019 | 18 minutes 29 seconds |
| Preprocessing alignments for candidate junction identification | 14:14:22 23 May 2019 | 14:17:43 23 May 2019 | 3 minutes 21 seconds |
| Preliminary analysis of coverage distribution | 14:17:43 23 May 2019 | 14:23:42 23 May 2019 | 5 minutes 59 seconds |
| Identifying junction candidates | 14:23:42 23 May 2019 | 14:26:06 23 May 2019 | 2 minutes 24 seconds |
| Re-alignment to junction candidates | 14:26:06 23 May 2019 | 14:35:39 23 May 2019 | 9 minutes 33 seconds |
| Resolving best read alignments | 14:35:39 23 May 2019 | 14:40:33 23 May 2019 | 4 minutes 54 seconds |
| Creating BAM files | 14:40:33 23 May 2019 | 14:45:05 23 May 2019 | 4 minutes 32 seconds |
| Tabulating error counts | 14:45:05 23 May 2019 | 14:47:06 23 May 2019 | 2 minutes 1 second |
| Re-calibrating base error rates | 14:47:06 23 May 2019 | 14:47:09 23 May 2019 | 3 seconds |
| Examining read alignment evidence | 14:47:09 23 May 2019 | 15:05:36 23 May 2019 | 18 minutes 27 seconds |
| Polymorphism statistics | 15:05:36 23 May 2019 | 15:05:36 23 May 2019 | 0 seconds |
| Output | 15:05:36 23 May 2019 | 15:06:59 23 May 2019 | 1 minute 23 seconds |
| Total | 1 hour 13 minutes 46 seconds | ||
