| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,372,484 | A→T | 26.1% | L431* (TTA→TAA) | pbuG ← | Guanine/hypoxanthine permease PbuG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,372,484 | 0 | A | T | 26.1% | 36.8 / 12.9 | 23 | L431* (TTA→TAA) | pbuG | Guanine/hypoxanthine permease PbuG |
| Reads supporting (aligned to +/- strand): ref base A (9/8); new base T (3/3); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
AATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGC > USA300TCH1516_ALE/2372347‑2372621 | aataTCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTtata < 2:300337/141‑1 (MQ=255) atCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATTGTACAATTattttat > 1:302226/1‑135 (MQ=255) gtaagatATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTa < 2:253789/135‑1 (MQ=255) aTGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGAATTTAACCATGAACAAATATCAAGTACAGAATGAATATTACCATTAATACATACATAATc > 2:45248/1‑141 (MQ=255) tGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCg > 1:313525/1‑141 (MQ=255) cGCTACTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTAct < 1:134839/141‑1 (MQ=255) ttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATTATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTa > 2:178839/1‑141 (MQ=255) aTTTGATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAGGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATg < 1:109868/141‑1 (MQ=255) tACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTagag < 1:277695/141‑1 (MQ=255) gtagtaaaCGTCTACCAAGCATTTGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCAtt < 2:40643/136‑1 (MQ=255) aaaaaaaCGACTACCAATCATTGGCAGTCGTTTATTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTg < 2:15518/141‑1 (MQ=255) aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTa > 1:210372/1‑141 (MQ=255) aaaaaCGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTATTAGAGATAAGAATTGTa > 2:130429/1‑141 (MQ=255) gccAATGATTGGTAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGa > 1:238452/2‑141 (MQ=255) aCCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGa < 1:199424/141‑1 (MQ=255) ccAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGaa > 1:232316/1‑141 (MQ=255) gTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAGTAAATCCacac > 1:63811/1‑141 (MQ=255) ttttGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATa > 1:253046/1‑141 (MQ=255) tgtgTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCa < 2:226063/141‑1 (MQ=255) tGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCa < 1:135340/141‑1 (MQ=255) aaCAAATATGAAGTATAGAAGGAGTGGTACCATTATAACATACATTATCAGTTGTACTTCTTTATGTTTTTTTGAGATAAGCATTGTAATTAGATAGAAAATCAATCCACACGCAATACCCGTTGCAATTGAATATGATaa < 2:319329/141‑1 (MQ=255) atatGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGca < 1:313696/141‑1 (MQ=255) atatGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGca > 2:15564/1‑141 (MQ=255) aatgaatAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATACCTCCACACGCAATACTAGTTGTCAACAAATATGATAAAGGCATCATAATGATTGt > 1:246553/1‑141 (MQ=255) aTTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGc > 1:220410/1‑141 (MQ=255) | AATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGC > USA300TCH1516_ALE/2372347‑2372621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |