Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,472	G→A	A67V (GCT→GTT)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,472	0	G	A	100.0%	121.6 / NA	35	A67V (GCT→GTT)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/9);  total (26/9)

TTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCC  >  USA300TCH1516_ALE/1744346‑1744609                                                                                                                               |                                                                                                                                          ttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCTAATTCTCATTGAATAAATTACATGACAGCCAATGATaa                                                                                                                      >  2:286218/1‑148 (MQ=255) ttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATaa                                                                                                                      >  2:51685/1‑148 (MQ=255) ttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATaa                                                                                                                      <  1:270010/148‑1 (MQ=255)         aTCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCAcgc                                                                                                              >  2:84545/1‑148 (MQ=255)          tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTCCAGGACAGCCAATGATAAAGCCCcgcc                                                                                                             >  2:142414/1‑147 (MQ=255)          tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCAcgcg                                                                                                             >  2:150676/1‑148 (MQ=255)          tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCAcgcg                                                                                                             >  2:141841/1‑148 (MQ=255)                tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg                                                                                                       <  2:277884/148‑1 (MQ=255)                tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg                                                                                                       <  2:16920/148‑1 (MQ=255)                tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg                                                                                                       <  1:153111/148‑1 (MQ=255)                  cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGt                                                                                                     <  2:151923/148‑1 (MQ=255)                     tGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                                                                  >  2:118837/1‑148 (MQ=255)                                ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATc                                                                                       >  2:115322/1‑148 (MQ=255)                                           tCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAATGATATCAACATCTCTGt                                                                            >  1:117766/1‑148 (MQ=255)                                                   aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCg                                                                    >  2:336144/1‑148 (MQ=255)                                                   aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCg                                                                    >  2:285613/1‑148 (MQ=255)                                                       tCATAACGAATGACTTCCCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTGTCTTTTGCGTAtt                                                                >  2:62903/1‑148 (MQ=255)                                                       tCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAtt                                                                >  2:260172/1‑148 (MQ=255)                                                         aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                              >  2:33419/1‑148 (MQ=255)                                                         aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                              >  1:43886/1‑148 (MQ=255)                                                         aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                              >  1:187584/1‑148 (MQ=255)                                                         aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGAGTATTCt                                                              >  1:285354/1‑148 (MQ=255)                                                                      gcgcGAGGGAATTTGCCTGCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                                                 <  2:78239/145‑1 (MQ=255)                                                                         cGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGTTAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg                                              <  1:209625/148‑1 (MQ=255)                                                                             gTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATa                                          >  2:282306/1‑148 (MQ=255)                                                                              tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAg                                         <  1:118837/148‑1 (MQ=255)                                                                                          ttttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCa                             >  2:257967/1‑148 (MQ=255)                                                                                            ttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc                           <  1:322243/148‑1 (MQ=255)                                                                                              cTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAt                         >  1:47208/1‑148 (MQ=255)                                                                                                    ggTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa                   >  2:336661/1‑148 (MQ=255)                                                                                                    ggTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa                   >  1:127362/1‑148 (MQ=255)                                                                                                               aaTCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTGAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTGGCAGAGCCATATGCTTCACCATTATCCATAATTgtagta        >  2:385241/1‑148 (MQ=255)                                                                                                               aaTCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgtagta        >  2:12848/1‑148 (MQ=255)                                                                                                                     cATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATCATTGTTGTTATATcc  >  1:366297/1‑148 (MQ=255)                                                                                                                     cATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATcc  >  1:191092/1‑148 (MQ=255)                                                                                                                               |                                                                                                                                          TTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCC  >  USA300TCH1516_ALE/1744346‑1744609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑