| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,744,472 | G→A | 100% | A67V (GCT→GTT) | apt ← | Adenine phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,744,472 | 0 | G | A | 90.7% | 80.5 / 0.4 | 32 | A67V (GCT→GTT) | apt | Adenine phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (13/16); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGT > USA300TCH1516_ALE/1744338‑1744599 | caccggctttCATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACa < 1:115360/133‑1 (MQ=255) cTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGa < 2:305666/140‑1 (MQ=255) tGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGTATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGAc < 2:66107/140‑1 (MQ=255) tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCa < 2:239497/140‑1 (MQ=255) tAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCa < 1:265025/140‑1 (MQ=255) tAATTGCATCTTTGTGCATTGTTAAAACACTAACACACTTTTCTAGGGCTTGCTCATAACGAATTACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCa < 1:240243/140‑1 (MQ=255) ttGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTAATTAAACTCAAGGACAGCCAATg > 1:557723/1‑140 (MQ=255) tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATcaa > 2:332787/1‑140 (MQ=255) tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTAAAGTACAGCCAATGATaaa < 2:70900/140‑1 (MQ=255) tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCCATGATAAAGc > 1:361376/1‑140 (MQ=255) tAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAg > 1:380033/1‑140 (MQ=255) aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGt > 1:435492/1‑140 (MQ=255) cATTTGTACCATATTGTAGGTCATACTCAGAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt < 2:210536/140‑1 (MQ=255) ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCCCGCGCTTCAGGTCCTACa > 1:491526/1‑140 (MQ=255) cATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGata < 1:247188/140‑1 (MQ=255) cTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAAAGGTGCAAAGCCAATCCACATTGAATAAACTACACGACAGACAATGATAACGCCAAGCGCTTCAGGTCCTACAACGATACAAACa > 2:509815/1‑140 (MQ=255) aGGTCATACTCATTACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtc < 2:301958/140‑1 (MQ=255) tCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATctct < 1:299886/140‑1 (MQ=255) aCTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTcc > 2:148562/1‑139 (MQ=255) cTCATACCGAATGTCTTGACGCGCTAATTACCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCCTTGAATAAACTACAGGCCTGCCAATGATAAAGGCACGCGCTTCAGGTCCCACAACGATATCAACATCTCTGTCtt < 2:182726/140‑1 (MQ=255) aaTGACTTCACGAGGTAATTTCCCTTCTTTTCTAGCAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAtt < 1:118212/140‑1 (MQ=255) aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTc > 1:330526/1‑140 (MQ=255) aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTACTACAACGATATCAACATCTCTGTCTTTTGCGTATTc > 2:23847/1‑140 (MQ=255) aTGACTGCACGTGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGCCTTTTGCGTATTc > 2:469311/1‑140 (MQ=255) ggTAATTTCCCTTCCTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATc < 1:120367/140‑1 (MQ=255) tttgcaaaCGGGTGCAAAGCCAACCCCCGTGGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCat < 2:51296/134‑1 (MQ=255) ttCTAACAGGTGCAAAGCCAATCTCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCatat < 2:421377/140‑1 (MQ=255) aCAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTc > 1:554568/1‑140 (MQ=255) ggTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATCGCCATATGCTTCAcc > 2:15594/1‑140 (MQ=255) tGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAt < 2:190058/140‑1 (MQ=255) tCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTATACAATTTTATCTGTTGCATAGCCATATGCTTAACCATTTTCCTTGAtt > 1:548628/1‑140 (MQ=255) cccATTGAATAAAATACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTATGTATTTTGCGTATTCTACAATTTTACATGTTGAATCGACCTATGTTTCATCATCATCCATAATTgt > 2:358808/1‑140 (MQ=255) | GACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGT > USA300TCH1516_ALE/1744338‑1744599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |