Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,744,472 G→A 44.6% A67V (GCT→GTT)  apt ← Adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,744,4720GA44.6% 14.3 / 37.2 44A67V (GCT→GTT) aptAdenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (8/15);  new base A (9/10);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATT  >  USA300TCH1516_ALE/1744345‑1744597
                                                                                                                               |                                                                                                                             
tttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAg                                                                                                                         <  1:167309/134‑1 (MQ=255)
 ttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTTCAAAGCCAATCCCCTTTGAATAAGCTACAgg                                                                                                                        >  1:121743/1‑134 (MQ=255)
   aaTTGTATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGAc                                                                                                                      <  1:483236/134‑1 (MQ=255)
   aaTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGAc                                                                                                                      >  1:118769/1‑134 (MQ=255)
         aTCTTTGTGCATTGTTAAAACATTTGTACCCTCCTCAACGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCaa                                                                                                                <  1:110614/134‑1 (MQ=255)
          tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATAACAATTGAATAAACTAACGGACAACCAAt                                                                                                               >  2:228362/1‑134 (MQ=255)
             ttGTGCATTGTTAAAACATTTGTATCATATTCTTGGTCATACTTATAACTAATGACTTCACGAGGTAATAACCCTGCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGAt                                                                                                            <  1:202506/134‑1 (MQ=255)
             ttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCaccgac                                                                                                            >  2:285241/1‑129 (MQ=255)
                    ttGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCa                                                                                                     <  1:88631/134‑1 (MQ=255)
                     tGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGCATAAACTACAGGACAGCCACTGATAAAGCCAc                                                                                                    >  1:519126/1‑134 (MQ=255)
                       ttAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATACACTCAcgc                                                                                                  >  1:16976/1‑134 (MQ=255)
                         aaaaCATTTGTAACATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCAAgcgc                                                                                                >  2:58607/1‑134 (MQ=255)
                               tttGTACCATATTCTAGGTCATATTCTTAACGAATGTCTTCACTAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAgg                                                                                          <  1:343511/134‑1 (MQ=255)
                                        tatTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAAc                                                                                 <  2:118769/134‑1 (MQ=255)
                                               ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAAGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCa                                                                          >  1:350876/1‑134 (MQ=255)
                                               ggTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTCAATAATCTACATGACAGCCCATAATCAACCCACGCACTTCAGTTCCTACAACGATATCa                                                                          >  1:130876/1‑134 (MQ=255)
                                                   aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGCTAAAGCCACGCTCTTCAGGTCCTACAACGTTCTCAAcct                                                                      >  1:27901/1‑132 (MQ=255)
                                                     aCTCATATCGAATGACTTCACGTAGTAATTCCCCTTCTTTCCTAACAGGCGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACAtct                                                                    <  1:579356/134‑1 (MQ=255)
                                                        cttAACTAATGACTTCACGTGGTAATTTTCCTTCTTTTCTAACAGGTGCAAAGCCAATCTCCATTGAATAAGCTACAGTACAGCCAATGATAGAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                 <  2:292280/132‑1 (MQ=255)
                                                         atcgcGAATGACTTCACGAGGTCATTTCCCTTCTTTGCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAAGACGTCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                <  2:602686/130‑1 (MQ=255)
                                                         aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCCTTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGAAATCAACATCTCTGt                                                                >  2:346726/1‑134 (MQ=255)
                                                         aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAAGTCAAACAACGATATCAATATATTTGt                                                                >  1:77780/1‑134 (MQ=255)
                                                         aTAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAACGATAAAGCCACGCGCTACAGGTCCTACAACGAAATCAACAtctcaca                                                                >  1:32544/1‑131 (MQ=255)
                                                                aTGACTCCACGAGGTCATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGc                                                         <  2:520253/134‑1 (MQ=255)
                                                                       cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                  <  2:532655/134‑1 (MQ=255)
                                                                       cACGAGGTAATGTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCTTAACACAATTGCAACATCTCTGTCTTTTGCGTATTCt                                                  <  2:131209/134‑1 (MQ=255)
                                                                                   tctctttttTTCTAACAAGTGCAAAGCCAATCCCAATTGAATAAACCACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCt                                      <  1:20735/131‑1 (MQ=255)
                                                                                   tCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCATCATCTCTGTCTTTTGCGTATTCTACAATTTTATCt                                      >  1:337237/1‑134 (MQ=255)
                                                                                   tCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCt                                      <  1:210342/134‑1 (MQ=255)
                                                                                       ttcttTTCTAACCGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg                                  <  1:362788/134‑1 (MQ=255)
                                                                                       ttcttTTCTAACAGGTGCAAAGCCAATAACTTCTTAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg                                  <  2:268017/134‑1 (MQ=255)
                                                                                          ttttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAATGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCAt                               <  1:455920/134‑1 (MQ=255)
                                                                                                agcatgTGCAAATCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCat                         <  2:447191/129‑1 (MQ=255)
                                                                                                aaCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCat                         <  1:426607/134‑1 (MQ=255)
                                                                                                aaCAGATGCAAAGCCAATCGCCATTTAATGATCGACAGGACAGCCAATGATAAAGCCACGCGCTTGAGGTCCTACACCGATATCAACATCTCTGTCTTTTGCGTCTTCTACAATTCTATCTGTTGCACGGCCat                         >  1:143995/1‑134 (MQ=255)
                                                                                                   aGGTGCAAAGCCAATCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATg                      >  1:337418/1‑134 (MQ=255)
                                                                                                    ggTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTATCGTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGc                     >  2:150142/1‑134 (MQ=255)
                                                                                                          aaGCCCAGCCCCATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc               <  2:519126/134‑1 (MQ=255)
                                                                                                                  ctccATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa       <  1:61946/132‑1 (MQ=255)
                                                                                                                  ccccATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCa       <  1:484801/134‑1 (MQ=255)
                                                                                                                  ccccATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTATATCTGTTGCATAGCCATATGATTCACAATTATCCa       >  2:403493/1‑134 (MQ=255)
                                                                                                                   cccATTGAATAAGCTACAAGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt      <  2:350876/134‑1 (MQ=255)
                                                                                                                    ccATTGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATa     <  1:332847/134‑1 (MQ=255)
                                                                                                                       ttGAATAAACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCACCCTCTCTGTCTTTTGCGTATTCTACACTTTTGTCTGTTGCATAGCCATATCCTCTGCCAATCTCCattatt  >  1:192260/1‑134 (MQ=255)
                                                                                                                               |                                                                                                                             
TTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATT  >  USA300TCH1516_ALE/1744345‑1744597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑