| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,279,705 | G→A | 100% | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,279,705 | 0 | G | A | 92.9% | 134.0 / ‑4.4 | 53 | A943V (GCA→GTA) | ebh_3 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (19/26); total (21/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
GTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATT > USA300TCH1516_ALE/2279582‑2279835 | gTTGACTCTATTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc < 1:305480/134‑1 (MQ=255) ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCa < 1:220565/134‑1 (MQ=255) ggttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGTCTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc < 1:478360/132‑1 (MQ=255) gATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc < 2:51992/134‑1 (MQ=255) gATTCTACTTCTGGTTTACTAGTTACATCGTTATCCAGTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc < 1:316196/134‑1 (MQ=255) aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGc > 2:131407/1‑134 (MQ=255) aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGATGTTTCAACTGTTGc > 1:375508/1‑134 (MQ=255) gtCTGGTTTACTAGTTACTTCGTTATCCAGTGTCGGATTGTTTGTTGATGCATCT‑ACACTAGCATTGTTATTAGCGTGCGCTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCg < 2:9989/133‑1 (MQ=255) tgtACTAGTTACATCGTTATACTCTGTAGGACTGTTTGATCAAGTATATAATATTATACTT‑TTCATAGTTCACGGTTTATCATTTGCATCATCAGTAGCTCAGGATTCTTTTCTTTCACTTGTTGCCGCATCAc < 2:460524/132‑1 (MQ=255) tACTAGTTACATCGTTATTCATTGTTGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTAGTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta < 1:410636/134‑1 (MQ=255) tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta < 1:96736/134‑1 (MQ=255) aGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGATTGCGGTTTATCATATGCATCATCAGTTGCTGATGTTAGTGTTAGTTTAGCTGCTGCCGCATCCAtattct > 2:381490/1‑132 (MQ=255) ttACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt > 1:385417/1‑134 (MQ=255) cGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgtt > 1:27908/1‑134 (MQ=255) tATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTATc > 1:248105/1‑134 (MQ=255) tCCATTGTCGGACTCTTTGTTGATGCATCT‑TCATTCTTATTTTTATTTGCTTCCGGTTTATCATGTGCAACATCAGGTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg < 1:4060/134‑1 (MQ=255) ttGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTGGCTGATGGTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGaa < 1:147404/134‑1 (MQ=255) cGGACTGTTTGTTTATGCATCT‑ACACTAGAATTGTTATTAGCTTGCTGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTCCTTGTAGTGGTCGGAGAAGCGt < 2:75033/134‑1 (MQ=255) acttcttgttgATGCATCT‑CCACTATAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg < 2:369618/129‑1 (MQ=255) ttgttgATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg < 1:407391/134‑1 (MQ=255) ttgATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTCTTTGGTGTTGTCGGAGCAGCGTCTTCTTTGCCa > 1:409734/1‑134 (MQ=255) tGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg > 1:494887/1‑134 (MQ=255) gCATTT‑ACGCTAGAGTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc < 2:608000/134‑1 (MQ=255) gCACCT‑ACGCTAGAATTGTGAGTCGCTTGCGGTTTAGCATTTGCATCATCAGTGGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc < 1:556757/134‑1 (MQ=255) tAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa < 1:114464/134‑1 (MQ=255) gtgattaGCTGGCGGTTTATCATTGGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTCGCTGTCGTCTCAGATACGt < 1:374635/131‑1 (MQ=255) gTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTAATGTTGTTTCACCTGTTGCCGCATAACTATTATTTGGTGTTGTCGGAGAAGCATCTGCTTGGCCATTAGCTGGCGTGTGAGATACGt > 2:85494/1‑134 (MQ=255) ttattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt < 1:92842/134‑1 (MQ=255) ttattaGCTTGCGGTTTATCATTTGCATCATCACTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt < 2:605668/134‑1 (MQ=255) ttaTCTTGCGGTTTATCATTTGCATCATCAGTTGGTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGTCATTAGCTGTCGTCTCAGATACGTTAgg < 1:258614/134‑1 (MQ=255) taGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGt > 1:228424/1‑134 (MQ=255) gCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg < 2:540872/134‑1 (MQ=255) gCTTGCGGTTTATCAGTTGCATCATCAGTTGCTTATGTTACTATTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTTAGATACGTTAGGTTg < 2:98194/134‑1 (MQ=255) tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGCTGTcc > 2:404113/1‑134 (MQ=255) cgttttATCATTTGCATCTTTAGTTGTTTATGTTCCTGTTGTTTCTCCTTTTGCCGCATTACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg < 2:203342/131‑1 (MQ=255) cgttttATCATTTACATCACCAGTTTCTGATGTTACTGTTGTTCGACGTGTTACCTCTTCTCTATTCGCAGGTGTGGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg < 2:590169/131‑1 (MQ=255) tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCTTTAGCTGTCGTCCCAGCTACGTTAGGTCGTTCAGTAttt > 1:36611/1‑134 (MQ=255) tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTGGTCTCAGATACGTTAGGTTGTCCAGTAttt > 2:48622/1‑134 (MQ=255) tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTAAGATACGTTAGGTTGTCCAGTAttt > 2:467491/1‑134 (MQ=255) tATCATTTGCATCATCAATTGCAGATGTTACTGTTGTTTCACCTGTTGCCGAATCACTATTAATTGGTGGTGTCGGAGAAGCGTCCGATTTGCAATTAGCTATCGACTCAGAGAAAATCGGTAGTCCAGtgagt > 2:412888/1‑130 (MQ=255) cgttGCTTCATTAGTTGCTGTTGTTGCTGTTGTTTCTCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGTCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg < 2:154421/132‑1 (MQ=255) aTTTGCATCATCAGTTGCTGATGTTATTGTTTTTTCACCTGTTGCCGCATCACTATTCTTTTGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGTTACGTTAGGTTGTCCAGTATTTTCTg < 2:181528/134‑1 (MQ=255) aTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCCTTAGCTGTCGTCTCAGATACGTTAGGCTGTCCAGTATTTTCTg > 1:70737/1‑134 (MQ=255) atcatcAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATAATTTGTTGGTGGCGGAGAAGCGTCTGCTTTGCCCTTAGCTGTCGTCTCAGATACGCTAGGTTGTCCAGTATTCTCTGGTGTTg > 2:9043/1‑134 (MQ=255) tcatcaGTTGCTAATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTCTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTATTGc > 2:285280/1‑134 (MQ=255) catcaGTTGCTGATGTTACTGTTGTTTCTCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGTATAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCa < 1:443425/134‑1 (MQ=255) tcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCCTCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATATTCTGGTGTTGCAtt > 1:592806/1‑134 (MQ=255) tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAt < 2:253172/134‑1 (MQ=255) tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATATGGTGTTGTCGGAGAAGAGTCTGCTTTGCCATTAGATGTCGTCTCAGATAAGTTACGTTGTCAAGTAGTGTCTGGTGTTGCATTAGCaa > 2:57688/1‑133 (MQ=255) cTGATGTTTCTGTTTTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt < 2:332913/134‑1 (MQ=255) gATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGa > 2:151886/1‑134 (MQ=255) gTTTCTGTTGTTGCACCTAGTGCCGCATCACTATTATTTTGTGTTGTAGAAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt < 1:223575/134‑1 (MQ=255) gTTGCTGTTGTTTGACGTGTTGCTGCATCCCTATTTTTTGGTGTTGTCGGAGTAGCGTGTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGTTGTTGCATTAGCATTTGAAtt < 2:506286/134‑1 (MQ=255) | GTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCT‑ACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATT > USA300TCH1516_ALE/2279582‑2279835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |