| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | USA300TCH1516_ALE | = 800928 | 25 (0.820) | 4 (0.140) | 4/266 | 3.2 | 16.4% | intergenic (+62/‑289) | hisC_1/USA300HOU_RS03915 | Histidinol‑phosphate aminotransferase/Putative 5'(3')‑deoxyribonucleotidase |
| ? | USA300TCH1516_ALE | 801948 = | 17 (0.590) | intergenic (+189/+435) | USA300HOU_RS03915/USA300HOU_RS03920 | Putative 5'(3')‑deoxyribonucleotidase/Putative lipid kinase | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
TGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTTGTAG > USA300TCH1516_ALE/800788‑801061 | tGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGAAAGAAATGATACTTTCGCAAAATTTATTTTGTCGTCccaacccaac > 2:18350/1‑141 (MQ=255) gtTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGCAGTTTTATCAAAAAAAAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTTGTCCCACCCCAACCTGc > 2:308024/1‑141 (MQ=37) gtTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAACTGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCAAACTTGc > 2:31528/1‑141 (MQ=255) aaTTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTAGCAAAATTTATTTCGTCATCCCACCCCAACTTGCATTGTCTGTaa > 2:119293/1‑140 (MQ=255) tGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTATGTAGaaa > 1:233265/1‑141 (MQ=255) ggTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCGCAACTTGCATTGTCTGTAGAAAt > 2:142681/1‑141 (MQ=255) ggTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAAt < 2:240219/141‑1 (MQ=255) tttAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTTTAGAAATTgg > 1:147170/1‑141 (MQ=255) aCAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCaa > 1:104323/1‑141 (MQ=255) tGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTtctc < 1:77437/141‑1 (MQ=255) gATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTctct > 1:22549/1‑141 (MQ=255) gTTGTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCccgc < 2:153878/141‑1 (MQ=255) tATCAAACTTTAAATACGAATAATAAGTTGGGAGTGGGACAGAAATCATACTTTCGCAAAATTTATTTCGTCGTCCTACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGc < 2:9242/141‑1 (MQ=255) tCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCaa > 2:152879/1‑141 (MQ=255) cAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAg > 2:195086/1‑141 (MQ=255) cAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAg > 2:60981/1‑141 (MQ=255) aactgcgaaTACGCATAGTAAGTGGGGAGTCGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTGGCATTGTCTGTAGAAAATGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGt < 1:27943/134‑1 (MQ=17) aaCTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGt < 1:158904/141‑1 (MQ=255) tACGAATAGGAAGCGCGGACTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCCACTTGCATTGTCTGTAGAAATTGGGAATCCAATGTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTCACTAGaa < 1:85353/141‑1 (MQ=14) aGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGGAATAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGCTGACTAGAAGTGaata > 1:167970/1‑139 (MQ=14) tgTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCACAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCCGCAAGGTTTACTAGCATTGAAAAAAGCTTGTTAc < 2:246593/140‑1 (MQ=255) gggACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAg > 1:211801/1‑141 (MQ=39) atttatttCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTg < 1:195086/141‑1 (MQ=32) atttCGTGGTCCTACCCTAAGTTGGATTGTGTCTAGAAATTGGGAATCCAATTTGTGTTTGTTGGGGCTCCGCCGGGAAGGTTGACGAGAATTGAAAAAAGCTTGGTACAAGCGCATTTTCGTTCAGTCAACTACTGCCaa < 2:238993/141‑1 (MQ=11) atttCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCCAGGTTGTCTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCaa < 1:128535/141‑1 (MQ=17) aacccAAGTTGCATTGTCGCGAGAAATTGGGAAACCAATTATTCTTTGTTGGGGCCCCGCCGACTAGGTTGAAAAAAAAAAAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTTGTag < 2:192424/139‑1 (MQ=11) | TGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTTGTAG > USA300TCH1516_ALE/800788‑801061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |