Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000731	81	N→G	intergenic (–/‑151)	– / → repA	–/replication protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000731	81	0	N	G	98.8%	265.8 / NA	81	intergenic (–/‑151)	–/repA	–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (39/41);  minor base C (0/1);  total (39/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGA  >  CP000731/1‑220                                                                                                                                          |                                                                                                                                            tatttatcaagtttttataaacccttaataaatctatattttcccttttataagtttATTTAAATTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGTGGTTTGGTAATAAAACGTTTAGaaa                                                                                                                                          >  2:271740‑M1/58‑141 (MQ=255)     tatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGAGGTTTGGTAATAAAACGTTTAGCAACTGt                                                                                                                                      <  2:247854‑M1/88‑1 (MQ=255)       tcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGATATAATAAACTTAATATAACAAAAACTCCAACTACTGGTAATCGTCGGGCTTTGGTAATAAAACGTATAGAAAATGttt                                                                                                                                    <  2:9121‑M1/90‑1 (MQ=255)       tcaagggtttataaacccttgataaagctatattgtcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGttt                                                                                                                                    >  1:335630‑M1/52‑141 (MQ=255)        caagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCACAACTAATGGTAATAATAGAGATTTGGTAATAAAAAGTTTAGAAACTGttta                                                                                                                                   <  2:105760‑M1/91‑1 (MQ=255)            ggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtttattta                                                                                                                               <  1:219468‑M1/95‑1 (MQ=255)            ggtttataaacccttgataaaattttatttttccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGtttattta                                                                                                                               >  2:87188‑M1/47‑141 (MQ=255)               ttatacacccaaaataaaaatagagttgcccttttataagtttATTTAAAGTGCTATAAAAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatat                                                                                                                            >  1:47644‑M1/44‑141 (MQ=255)               ttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTtatat                                                                                                                            >  1:76235‑M1/44‑141 (MQ=255)                tataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTATTGGTAATAGTCGGGGTTTGGTAGTACAACGTTTAGAAAGTGTACATTaatata                                                                                                                           <  1:164897‑M1/99‑1 (MQ=255)                  taaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAt                                                                                                                         <  1:252825‑M1/101‑1 (MQ=255)                    aacccttgataaagctatggtttttcttttataagtttATTTAAAGTGCTATAATAAACTTACTATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                       <  2:129304‑M1/103‑1 (MQ=255)                    aacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAGACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                       >  1:224586‑M1/39‑141 (MQ=255)                    aacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                       >  2:307056‑M1/39‑141 (MQ=255)                      cccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTc                                                                                                                     <  1:262958‑M1/105‑1 (MQ=255)                      cccttgataaagctatattttcccttttataaatttATTTAAAGTGCTATAATAAACTTAATATACCAAAAACCCCAACTACTGGTAATAGTCGGTGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTc                                                                                                                     <  2:7849‑M1/105‑1 (MQ=255)                       ccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCg                                                                                                                    >  1:1305‑M1/36‑141 (MQ=255)                       ccttgataaagctatattctcccgtttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAATCCCCAACTTCTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCg                                                                                                                    >  1:59110‑M1/36‑141 (MQ=255)                        tttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAATTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTttcaa                                                                                                                   >  2:271628‑M1/35‑139 (MQ=255)                         ttgataaagctattgtttcccttttataagtttATGTAAAGTGTTATAATAAACTTAATATAACAAAAACCCCAACTACTTGTCATATTCGGGGTTTGGTTATAAAACGTTGAGAAACTGTTTATTTATATAATTTTCGAt                                                                                                                  >  2:221036‑M1/34‑141 (MQ=255)                           gataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTAGATAc                                                                                                                <  2:70594‑M1/110‑1 (MQ=255)                           gataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTAGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATAc                                                                                                                <  2:165795‑M1/110‑1 (MQ=255)                            ataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATacc                                                                                                               <  1:26575‑M1/111‑2 (MQ=255)                               aagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGt                                                                                                            >  1:244090‑M1/28‑141 (MQ=255)                                      attttcccttttataagtgtATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCACAACTGCTGGTAATAGTCGTGGTTTGTTAATAAAACGTATAGAAACTGTTTATTTATATAATTTTCGATACTTGTATtatat                                                                                                     >  2:166565‑M1/21‑141 (MQ=255)                                       ttttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATtatata                                                                                                    <  1:124076‑M1/122‑1 (MQ=255)                                       ttttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAAGAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATGCTTGTATtatata                                                                                                    <  1:190898‑M1/122‑1 (MQ=255)                                          tcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATg                                                                                                 >  2:116401‑M1/17‑141 (MQ=255)                                            ccttatataagtttATTTAAAGTGCTATAATAAACATAAAATAAAAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaa                                                                                               >  2:40602‑M1/15‑141 (MQ=255)                                             tttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaat                                                                                              >  2:1288‑M1/14‑141 (MQ=255)                                               tttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGaataa                                                                                            >  2:40284‑M1/12‑141 (MQ=255)                                               tttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGAGACTTGTATTATATAATGaataa                                                                                            >  2:307976‑M1/12‑141 (MQ=255)                                                     agtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCtt                                                                                      <  1:247292‑M1/136‑1 (MQ=255)                                                     agtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCtt                                                                                      <  1:272137‑M1/136‑1 (MQ=255)                                                       tttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAg                                                                                    >  2:330175‑M1/4‑141 (MQ=255)                                                         tATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCTATACTTGTATTATATAATGACTATTATcttattt                                                                                  <  2:228723‑M1/140‑4 (MQ=255)                                                                aGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTACTAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                           >  1:277161/1‑141 (MQ=255)                                                                aGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                           >  1:326805/1‑141 (MQ=255)                                                                aGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                           >  1:191484/1‑141 (MQ=255)                                                                aGTACTATAATAAACGTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                           <  1:294083/141‑1 (MQ=255)                                                                 gTGCTATAATAAACTTATATTAACAAAAACCGTAATTATTGGTAATGGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                          <  2:124650/141‑1 (MQ=255)                                                                 gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                          <  1:212112/141‑1 (MQ=255)                                                                 gTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                          <  1:320541/141‑1 (MQ=255)                                                                         ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCTGTGTTTAGTTCTTAAACGTTGTCAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                                  <  2:76022/141‑1 (MQ=255)                                                                         ataaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                                  <  2:204232/141‑1 (MQ=255)                                                                           aaaCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATta                                                                <  1:124396/141‑1 (MQ=255)                                                                                         aaaaaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGc                                                  <  1:14998/141‑1 (MQ=255)                                                                                          aacaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCt                                                 <  1:287888/138‑1 (MQ=255)                                                                                            aaCCCCAACTACTGGTAATAGTCGGGGTTTGATAATAAAACGTTTAGAAACTGTTTATTGATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaa                                               >  2:48209/1‑141 (MQ=255)                                                                                              ccccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                             >  2:30885/1‑141 (MQ=255)                                                                                               cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                            >  2:314701/1‑141 (MQ=255)                                                                                                ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                           <  1:99429/141‑1 (MQ=255)                                                                                                ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                           <  1:280549/141‑1 (MQ=255)                                                                                                ccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                           >  1:341822/1‑141 (MQ=255)                                                                                                    ttactGGTAATAGTCGGGGTTTAGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAAc                                       <  2:255833/140‑1 (MQ=255)                                                                                                     aactGGTAATATTCGGGGTTTGGTAATAAAACGTTTAGATTTTATCAGTGTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACg                                      <  2:159398/140‑1 (MQ=255)                                                                                                      actGTTAGTAGTCGGGGTTTGGTAATAAATCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                     <  2:296588/141‑1 (MQ=255)                                                                                                      actGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                     >  1:131724/1‑141 (MQ=255)                                                                                                      actGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                     >  1:42126/1‑141 (MQ=255)                                                                                                      actGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                     >  1:305353/1‑141 (MQ=255)                                                                                                      actGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGt                                     >  2:19190/1‑141 (MQ=255)                                                                                                         ggTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGtttt                                  >  1:325661/1‑141 (MQ=255)                                                                                                         ggTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGtttt                                  >  1:215401/1‑141 (MQ=255)                                                                                                           tAATAGTCGGGGTTTGGTAATCAAAAGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTttat                                <  1:70270/141‑1 (MQ=255)                                                                                                              tcgagagggCTTGGTAATAAACCGTTTAGAAACTGTTTATTTATGTAGTGGACACACCTTAAACGTCATAATGGATAATATCTTAGTGAAAGCACCTAAATCATTAAATAAATGTAAAACTAAAAAAAAACGTTTTATTAc                             >  2:344962/7‑141 (MQ=255)                                                                                                                 taggtgTTTTGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAACGAATCGGAGTACAGTACACGATAAGATATCACGAAATAAATGTAAAGATAAATTAAAGCGTTTTATTACctc                          <  2:252526/136‑1 (MQ=255)                                                                                                                 tCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACctc                          <  1:187968/141‑1 (MQ=255)                                                                                                                 tCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACctc                          <  1:52065/141‑1 (MQ=255)                                                                                                                   ggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAATTAAATAAACACGTTTTATTACCTCtt                        >  2:322768/1‑141 (MQ=255)                                                                                                                     gggttGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAc                      <  1:128821/138‑1 (MQ=255)                                                                                                                      gTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAcc                     <  1:247854/141‑1 (MQ=255)                                                                                                                        ttGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaa                   <  2:272137/141‑1 (MQ=255)                                                                                                                        ttGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaa                   <  2:247292/141‑1 (MQ=255)                                                                                                                         ttgTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCCACACTTGTATTATATAATGAATAATATCTTCGTGCATGCTCCTGAATCTTTAAAGAAATGTAAAGCTGAATAAACACGTTTTATTACCTCTTACCaaa                  <  2:252825/139‑1 (MQ=255)                                                                                                                          ggTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaaa                 >  2:294396/1‑140 (MQ=255)                                                                                                                               taaAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaaaa            >  1:141016/1‑141 (MQ=255)                                                                                                                               taaAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTaaaa            >  2:16978/1‑141 (MQ=255)                                                                                                                                aaaaCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAat           <  2:38619/141‑1 (MQ=255)                                                                                                                                   aCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATg        <  2:25357/141‑1 (MQ=255)                                                                                                                                       ttAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGaaa    >  1:302365/1‑141 (MQ=255)                                                                                                                                         aGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAACCTAAAATATGGAAaga  >  1:130562/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                            ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAGA  >  CP000731/1‑220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑