Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,648,014	C→T	D151N (GAT→AAT)	lipM ←	Octanoyltransferase LipM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,648,014	0	C	T	100.0%	73.4 / NA	24	D151N (GAT→AAT)	lipM	Octanoyltransferase LipM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

ATCAAACAGTTCATCAATATCAATATCTTGTAAAATCGAACCGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATCAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATCAAATCCTAAGTTTTTAAATCCTTCTAATAATCCTTGTGAAATCACTCTGTACGCTTCTGTA  >  USA300TCH1516_ALE/1647883‑1648150                                                                                                                                    |                                                                                                                                         atCAAACAGTTCATCAATATCAATATCTTGTAAAATCGAACCGTGTTGGAGGATTACGCCC‑TTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACt                                                                                                                                >  1:293418/1‑141 (MQ=255)    aaaCAGTTCATCAATATCAATATCTTGTAAAATCGAACCGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGATCATACCAACTTGGTGCATTAAAACACACTGa                                                                                                                              >  2:125822/1‑141 (MQ=255)             atcaagatcaatATCTTGTAAAATCGAACCGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAgg                                                                                                                     <  2:72187/141‑1 (MQ=255)                    tcaatatcTTGTAAAATCGATCCGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGtttt                                                                                                              <  2:87355/141‑1 (MQ=255)                    tcaatatcTTGTAAAATCGAACCGTGTTGGAGGATTACGCCCTTTTGTCGCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGtttt                                                                                                              <  2:117205/141‑1 (MQ=255)                       atatcTTGTAAAATCGAACCGTGTTGGAGGATTACGCCCTTTTGTCTAGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACGCACTGACCTGCCACGTTCTTTTata                                                                                                           >  2:221467/1‑139 (MQ=255)                         atcTTGTAAAATCGAACCGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTCAtct                                                                                                         >  2:346527/1‑139 (MQ=255)                                    tCGAACCGTGCTCGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCtt                                                                                              <  1:132244/141‑1 (MQ=255)                                     cGAACCGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTc                                                                                             <  1:211016/141‑1 (MQ=255)                                        aCCGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCACCTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAgg                                                                                          >  1:195331/1‑141 (MQ=255)                                          cGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCg                                                                                        >  2:257892/1‑141 (MQ=255)                                            tgtTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGtt                                                                                      <  2:294219/141‑1 (MQ=255)                                                  aggaTTACGTCCTTTTGTCTCGTTTGATCACTCCCATCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTATCCATCTTCAGGTGTTTTAGGt                                                                                <  2:129752/141‑1 (MQ=255)                                                          gCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCaaa                                                                        <  1:107475/141‑1 (MQ=255)                                                             cTTTTGTCTCGTTTGAGCACTCGCAGCAATCTTACGGACTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAAGCGTTTTAAGTACCGCAAAATa                                                                     >  2:53367/1‑141 (MQ=255)                                                                   tctcGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATc                                                               >  1:106225/1‑141 (MQ=255)                                                                   tctcGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATc                                                               >  1:18758/1‑141 (MQ=255)                                                                               aCTCCTAGTAATCTTACGGAATTCAACAACGATCTCATACCAACTTGGTGCATTAAAACAAACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATCAAATCCTAAGtt                                                   <  2:322971/141‑1 (MQ=255)                                                                                                           acTAGCTCATACCAACGTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATGAAATCCTAAGTTTCTGCATCGTTCTAATCATCCTTGTCaa                       >  2:170713/1‑141 (MQ=255)                                                                                                            cTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATCAAATCCTAAGTTTTTAAATCCTTCTAATAATCCTTGTGaaa                      <  2:106225/141‑1 (MQ=255)                                                                                                            cTAGCTCATACCAACTTGGTGCATTAAAACACACTGAACTTAGAGGTTGTTATAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATCAAATCCTAAGTTTTTAAATCATTCTAATAATCCTTGTGaaa                      >  1:129319/1‑141 (MQ=255)                                                                                                                   aTACCAACTTGGTGCATTAAAACACACTGAACTTCGCGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATCAGTATCAAATCCTAAGTTTTTAAATCCTTCTAATAATCCTTGTCAAATCActct               >  2:253186/1‑141 (MQ=255)                                                                                                                        aaCTTGGTGCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATCAAATCCTAAGTTTTTAAATCCTTCTAATAATCCTTGTGAAATCACTCTGTACg          <  1:257892/141‑1 (MQ=255)                                                                                                                                gCATTAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATCAAATCATCAGTTAATTGATCCTTCTAATAATCCTTGTGAAATCACTCTGTACGCTTATGTa  >  2:159561/1‑141 (MQ=255)                                                                                                                                    |                                                                                                                                         ATCAAACAGTTCATCAATATCAATATCTTGTAAAATCGAACCGTGTTGGAGGATTACGCCCTTTTGTCTCGTTTGAGCACTCCCAGCAATCTTACGGCCTTCAACAACTAGCTCATACCAACTTGGTGCATCAAAACACACTGAACTTCGAGGTTGTTTTAATTTTTGACGCTCTTCAGGCGTTTTAGGTACCGCAAAATAAGTATCAAATCCTAAGTTTTTAAATCCTTCTAATAATCCTTGTGAAATCACTCTGTACGCTTCTGTA  >  USA300TCH1516_ALE/1647883‑1648150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑