| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 517,732 | T→C | 29.7% | intergenic (+5800/‑963) | recR → / → speA_2 | Recombination protein RecR/Arginine decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 517,732 | 0 | T | C | 29.7% | 60.5 / 66.4 | 88 | intergenic (+5800/‑963) | recR/speA_2 | Recombination protein RecR/Arginine decarboxylase |
| Reads supporting (aligned to +/- strand): ref base T (33/28); new base C (8/18); total (42/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01 | |||||||||||
CTGACGAATACTAATCGATCGAAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGTCTCTTTTTTTATGTCTAAA > USA300TCH1516_ALE/517593‑517871 | cTGACGAATACTAATCGATCGAAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTc < 2:77677/141‑1 (MQ=31) cGAATCCTACTCGACCGAAGACTTAGTCAAAATAAATGTTTAGCGAAGCAAAAACAGTATTACTAACAATAGAGTTTTGAATGTATAAATTACATGCATATGTCGGGTCGTTACATCAAAGAGG‑TCACACCAGTTTCCATg > 2:311668/1‑141 (MQ=2) aCTAATCGATCGAAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACCTTAACTTACTGTCTAGTTTTGAATGTCTAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGaa < 1:368850/141‑1 (MQ=255) cTAATCGATCGAAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGCCTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAac > 1:261525/1‑141 (MQ=17) tcgatcgaAGACTTAATCAAAATGAATGGTTTGCGAAGGAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATGCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAg < 2:214863/141‑1 (MQ=255) tcgatcgaAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGCGG‑TCACACCTGTTTCCATGCTGAAcacaa > 2:533636/1‑140 (MQ=17) tcgatcgaAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTAATAGCTATTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TAACACCTGTTTCCATGCCGAACACAg > 2:318164/1‑141 (MQ=255) gaAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTa > 2:324486/1‑141 (MQ=32) gaAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTAACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACTCAGAAGTTa > 2:419482/1‑141 (MQ=17) ataCTTAATCAAAATAAATGTTTGGCGAAGTAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAg < 2:101796/139‑1 (MQ=12) cTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAATTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGACGAAGACAGAAGTTACGCtt > 2:230580/1‑140 (MQ=255) tAATCAACATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGGTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCt < 1:231062/141‑1 (MQ=17) tAATCAAAATAAATGTTTTGCGAAGCAAAATGACTTGTGCCTCAGACTTTGGTTTGAATGTATAAAGTACATTCACATGTCTGGTGACTATAGCAAGGAGG‑GCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCt < 2:453553/141‑1 (MQ=11) aaTCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAATTAGGCTCA‑AGCCGCCTTCTTGCTGAACACAGAAGTTAAGCTCCtt > 1:88518/1‑141 (MQ=11) tCAAAATAAATGTTTTGCGAAGCAAACTGACCCTCAGCTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAg > 2:523561/1‑141 (MQ=11) tCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAg > 1:360751/1‑141 (MQ=32) aaaaTAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCg > 2:58150/1‑141 (MQ=32) aatGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCCTAGCGTCCATg > 2:296153/1‑141 (MQ=17) aatGTTTTGCGAAGCAAAATCACTTTTACTGACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATg < 1:199313/141‑1 (MQ=17) gTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTa > 1:258130/1‑141 (MQ=32) gCGAAGCAAAATCAGCTTGCGTTACTAGCTCGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGa < 2:194898/141‑1 (MQ=11) aaGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACt < 1:5602/141‑1 (MQ=32) aGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACtt > 1:582134/1‑141 (MQ=32) tCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTGGTCGAACTTACGTTcc < 2:509361/141‑1 (MQ=17) aCTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGc > 2:294226/1‑141 (MQ=32) aCTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGc < 1:636491/141‑1 (MQ=32) ttactATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAg < 2:257389/141‑1 (MQ=32) gtATCCAGTTTTGAATGTATACACGACATTCATATGGCTGGTGACTATAGCCAGGAGG‑AGTCACCTGTTTCCATGCCGAACACAGATGTTAAGCTCCTTAGCGCCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAAc < 1:56629/140‑1 (MQ=11) ctATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAAc < 2:14688/141‑1 (MQ=32) ctATCTAGTTTTCGATGTATAAAGTACATTCATATGGCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAAc < 2:255173/141‑1 (MQ=255) tCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTCACGTACCGCCAGAGTAGAACGtt > 2:415901/1‑141 (MQ=12) gAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCaa > 1:281858/1‑141 (MQ=32) aaTGTATAAATTACAGTCATATGTCGGGTGACTATAGCAAGGAGG‑TCACACCTGTTGCCATGCCGAACAAAGACGTAAAGTTTCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgt > 2:196383‑M2/1‑139 (MQ=255) aTGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCaaaa < 1:523561/141‑1 (MQ=32) gTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCaaaaaa < 1:620451/141‑1 (MQ=32) tataAATTACATTCAGATGTCTGGTGACTATAGCAAGGAGG‑TCGCACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAAt < 2:605778/141‑1 (MQ=17) aaaTTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGa < 1:531430/141‑1 (MQ=32) aaaTTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGa < 1:58150/141‑1 (MQ=32) aaaTTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCGGGCAgttttttaa < 2:385822‑M2/141‑10 (MQ=255) aaTTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaat > 1:560040‑M2/1‑131 (MQ=255) aaTTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaat > 1:195335‑M2/1‑131 (MQ=255) actACAGTCATATGTCTGGTGACTATAGCAGGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGCTGGTAGTCGAACTTACGTTCCGGTAGAAGAGAACGTTGCCAGGCAAAAAATGGATg < 2:232458/139‑1 (MQ=11) aTTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatc > 1:571091‑M2/1‑130 (MQ=255) aTTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatc < 2:340041‑M2/141‑12 (MQ=255) cATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAATTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCa < 1:70112/126‑1 (MQ=18) cATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAATTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCa > 2:70112/1‑126 (MQ=18) cATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaat < 1:614036‑M2/141‑16 (MQ=255) cATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaat < 1:406378‑M2/141‑16 (MQ=255) ttCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGACCGTTGCCAGGCAgttttttaatcaacttt < 1:253855‑M2/141‑18 (MQ=255) cATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTACGCTCCTTAGCGTCGATGGTAGTCCAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttg < 2:107183‑M2/141‑20 (MQ=255) tatGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggt < 1:417465‑M2/141‑22 (MQ=255) cTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCCCAAAATAGAGTCGATGAACCgacttg > 2:384602/1‑136 (MQ=11) cTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTg > 1:241028/1‑141 (MQ=32) cTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTCGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaa < 2:579600‑M2/141‑27 (MQ=255) cTGGTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaa > 1:590777‑M2/1‑115 (MQ=255) ggTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTCGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaaaa > 1:457934‑M2/1‑113 (MQ=255) gTGACTATATCAAGGAGG‑TCACATCTGTTTCTATGCAGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCATGCAAAAAATGGATGCGATGAGCCGCATTgaga < 2:333257/141‑1 (MQ=11) gTGACTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCATGCAgttttttaatcaaattttggttaaaaaat > 1:617027‑M2/1‑112 (MQ=255) gACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGAcc > 1:140624/1‑141 (MQ=32) cTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTCAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGAATGCGATGAGCCCCATTCAGActcc > 2:413749/1‑138 (MQ=11) cTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACAAAGAAGTTAAGCTCCTTAGCGCGGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAAGGGATGCGATGAGCCGCATTGAGACCGc > 1:622966/1‑141 (MQ=255) cTATAGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaaaataaaa > 1:205386‑M2/1‑108 (MQ=255) taGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAg > 1:33802/1‑141 (MQ=32) taGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACATTGCCAGGCAGAAACGGGAAGCGATGAGCCGCATCTAGACCGCAAg > 2:326912/1‑141 (MQ=11) taGCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaaaataaaatgg < 1:638392‑M2/141‑37 (MQ=255) aGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCTTCTATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATTAGCCGCATTGAGACCGCAAgg < 1:404079/141‑1 (MQ=12) aGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAgg < 1:96843/141‑1 (MQ=32) gCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTCGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgctttttaattaacttttggttaaaaaataaaatggac < 2:567068‑M2/141‑39 (MQ=255) gCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGCACGTTGCCAGGCAgttttttaatcacattttggcaaaaaaatataattgaa < 2:475811‑M2/141‑39 (MQ=255) gCAAGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaaaataaattggac < 1:549665‑M2/141‑39 (MQ=255) aaGGAGG‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgtcttttaatcaaattttggttaaaaaataaaatggacaa < 2:245886‑M2/141‑41 (MQ=255) aggagg‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGtctc < 1:324486/141‑1 (MQ=32) aggagg‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGtctc > 1:488441/1‑141 (MQ=32) ggagg‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaaaataaaatggacaaga > 1:332255‑M2/1‑99 (MQ=255) gagg‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGAGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGTCTCtt < 2:289488/141‑1 (MQ=17) gagg‑TCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaaatttggttaaaaaataaaatggacaagat < 1:233486‑M2/141‑44 (MQ=255) agg‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCCTTGAGACCGCAAGGtacattt > 2:507516/1‑135 (MQ=12) agg‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGTCTCttt > 2:478533/1‑141 (MQ=32) agg‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGTCTCttt > 2:230537/1‑141 (MQ=32) gg‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTCGCGTCCCAGGACCCACCCCCCAGGGGCCGCTAGAGTATAACGTTGCCCGGCAAAAAATGCATGCGATGAGCCGCATTGAGACCGCAAGGTCTCtttt > 1:304620/1‑141 (MQ=2) tCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGCACTTACGTTCCGCTAGAGTAGAACGTTGCAAGGCACAAAATGGATGCGATGAGCCGCATTGAACCCGCAAGGTCTAttgatt > 2:570495/1‑137 (MQ=11) tCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGTCTCtttttt > 2:55520/1‑141 (MQ=32) tCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCAGCATTAAGACCGCAAGGTCTCtttttt > 2:306910/1‑141 (MQ=17) tCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttcaaaaataaaatggacaagataaaa < 1:18343‑M2/141‑48 (MQ=255) tCACACCTGTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACGTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaaaataaaatggacaagataaaa < 2:485078‑M2/141‑48 (MQ=255) cTGTTTCCATGCCAAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATCAGCCGCACTGAGACCTCAATGTCTCTTTTTTTATGTc < 1:504992/141‑1 (MQ=11) gTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGCACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaaaataaaatggacaagataaaaaaagttat < 2:247762‑M2/141‑56 (MQ=255) gTTCCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAgttttttaatcaaattttggttaaaaaataaaatggacaagataaaaaaagttat < 1:526673‑M2/141‑56 (MQ=255) tttCCTGGCGGACCACAGTAGTGCAGCTGCTTAGAGTCGATGGTAGTCGAACTTACGGTCCGCTCGATGAGAACGTTGCCAGGCAAATAATGGATGCGATGAGCGGCATTGAGACCGCAAGGTCTCTTTTTTTATGTCTaa < 1:186130/141‑1 (MQ=11) tttCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGTCTCTTTTTTTATGTCTaa < 1:502682/141‑1 (MQ=35) ttCAATGCCGAACACTGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGCATGCGATGAGCCGCATTGAGACCGCAAGGTCTCTTTTTTTATGTCTaaa < 2:106141/141‑1 (MQ=12) | CTGACGAATACTAATCGATCGAAGACTTAATCAAAATAAATGTTTTGCGAAGCAAAATCACTTTTACTTACTATCTAGTTTTGAATGTATAAATTACATTCATATGTCTGGTGACTATAGCAAGGAGG‑TCACACCTGTTTCCATGCCGAACACAGAAGTTAAGCTCCTTAGCGTCGATGGTAGTCGAACTTACGTTCCGCTAGAGTAGAACGTTGCCAGGCAAAAAATGGATGCGATGAGCCGCATTGAGACCGCAAGGTCTCTTTTTTTATGTCTAAA > USA300TCH1516_ALE/517593‑517871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |