Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,512	A→G	34.6%	S194S (TCA→TCG)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,512	0	A	G	34.6%	73.4 / 47.9	66	S194S (TCA→TCG)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (22/21);  new base G (11/12);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAA  >  USA300TCH1516_ALE/111374‑111650                                                                                                                                            |                                                                                                                                           tACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGc                                                                                                                                          >  1:598279/1‑141 (MQ=255)  aCTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCt                                                                                                                                         >  1:215776/1‑141 (MQ=255)   atGAGGAAGTTAAAAAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCta                                                                                                                                        <  1:265140/140‑2 (MQ=255)    tGAGGAAGTTAAAGAAGCAAACATAAAAAACGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGATGC‑CGAGTTATTCGGCtaa                                                                                                                                       >  2:560874/1‑139 (MQ=255)    tGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCtaa                                                                                                                                       <  1:575032/141‑3 (MQ=255)     gAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTaaa                                                                                                                                      >  1:215208/1‑141 (MQ=255)          aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGCTATTCGGCTAAATATCa                                                                                                                                 >  2:335322/1‑141 (MQ=255)              aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTa                                                                                                                             >  1:222809/1‑141 (MQ=255)              aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTa                                                                                                                             >  1:13005/1‑141 (MQ=255)                ggaggaaAAGATAAAAAAAGAAATCGAAAACTTTAAGTTTATTGCGCAATATAGCAATTTGAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                           <  1:615246/137‑1 (MQ=17)                 gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGTGC‑CGAGTTACTCAGCTAAATATCAATTAACt                                                                                                                          >  2:115445/1‑141 (MQ=255)                    gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                       <  1:303912/141‑1 (MQ=255)                    gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                       <  1:355742/141‑1 (MQ=255)                     aaacaaTAAAAAATGAAAT‑GAAAAATTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                                                                     <  2:200340/136‑1 (MQ=17)                      acaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                                                                     <  1:461766/139‑1 (MQ=255)                       aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGCGCTACAGCATACAATCTAGAGGTACTCGTCTTCTT‑AGCTCAATATCAATTAACTAatgat                                                                                                                    >  1:242937/1‑141 (MQ=21)                         cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                  >  2:409899/1‑141 (MQ=255)                         cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAAtgatga                                                                                                                  >  1:198437/1‑141 (MQ=255)                          aTAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                                                 >  1:306293/1‑141 (MQ=255)                            aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                                               >  2:383768/1‑141 (MQ=255)                             aaaagaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                              >  2:461408/1‑141 (MQ=255)                             aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                              >  2:148593/1‑141 (MQ=255)                             aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                              >  2:342707/1‑141 (MQ=255)                             aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                              <  2:604620/141‑1 (MQ=255)                                aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAt                                                                                                           >  2:27208/1‑141 (MQ=255)                                aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑AGAGTTATTCGGCTAAATATCAATTAACTAATGATAATTATAAt                                                                                                           >  2:432826/1‑141 (MQ=255)                                aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATAACATCATTCAGAGTTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAt                                                                                                           <  1:92744/141‑1 (MQ=21)                                 aaGATATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATg                                                                                                          <  2:284571/141‑1 (MQ=255)                                  aGAAATCGAAAACTTTAAGTTTTGTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGt                                                                                                         <  1:391804/141‑1 (MQ=255)                                     aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCAAGAGTTGC‑TGAACTATTCGTCTACAAATCAATTAACTACTCATAACGATAATGTaca                                                                                                      >  2:3559/1‑139 (MQ=255)                                     aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATAAAGAGGTGC‑TGAGTTATTCGGCTAAATATCAATTAACTAATTATTATTATAATTTaaa                                                                                                      >  2:4905/1‑141 (MQ=17)                                      aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                     >  1:215142/1‑141 (MQ=255)                                       tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAc                                                                                                    >  1:601640/1‑141 (MQ=255)                                       tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAc                                                                                                    >  1:486477/1‑141 (MQ=255)                                              cTTTAAGTTTTTTGAGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTAATCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                                             <  2:262813/141‑1 (MQ=255)                                              atttAAGATTTTTAAAGAATATATTATTTGTAAAAATTTAATGAATTATAAAGATGAAGATATATAATATAATACAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                                                             <  2:257117/140‑1 (MQ=11)                                                 tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaa                                                                                          <  1:539947/141‑1 (MQ=255)                                                    gTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                                                                       <  1:11843/141‑1 (MQ=255)                                                       ttttGAGCACTATAGCAATTTTAAAGATTTAGTGAGTTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCCATTAACTAATGATGATTATAATGTAAAACAATTACGCCAAAGata                                                                                    >  2:215824/1‑141 (MQ=14)                                                        tttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGatat                                                                                   >  1:521945/1‑141 (MQ=255)                                                            cgcCATATAGCAATTTTAAAGATTTAATGAAATATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGata                                                                               <  1:63916/141‑3 (MQ=14)                                                             gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                              <  1:183501/141‑1 (MQ=255)                                                                   taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgac                                                                        <  1:417282/141‑4 (MQ=14)                                                                   taGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaac                                                                        <  2:402198/141‑1 (MQ=255)                                                                          tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                 <  2:115760/141‑1 (MQ=255)                                                                                   ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACGAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                        <  2:186683/141‑1 (MQ=255)                                                                                      aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                     >  1:1627/1‑141 (MQ=255)                                                                                       tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                    <  1:235504/141‑1 (MQ=255)                                                                                       tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                    <  1:586474/141‑1 (MQ=255)                                                                                        gAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                                   >  2:186624/1‑141 (MQ=255)                                                                                              taAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaa                                             <  1:514640/141‑1 (MQ=255)                                                                                               aaaGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                            <  2:395982/141‑1 (MQ=255)                                                                                                 aGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGCATATCCACAACAACAGTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                          >  1:634761/1‑141 (MQ=17)                                                                                                 aGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCCACAAATAAAGCGCCGAAGCTGTTGTTGAACgg                                          >  1:173256/1‑141 (MQ=255)                                                                                                             atCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAttt                              <  2:13005/141‑1 (MQ=255)                                                                                                               cATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaa                            <  2:290154/141‑1 (MQ=255)                                                                                                                   taATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATACAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg                        >  2:314200/1‑141 (MQ=255)                                                                                                                   taATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg                        <  2:477874/141‑1 (MQ=255)                                                                                                                       ccAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                    <  2:242937/141‑1 (MQ=255)                                                                                                                       ccAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGGtca                    >  1:629520/1‑141 (MQ=255)                                                                                                                        gagagGGGC‑CGAGTTAGTCAGGGAGATATGAGTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAGCAACAAATAGAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtatgg                   <  2:636130/140‑5 (MQ=14)                                                                                                                             gTGC‑CGGGTTATTCAGCACAAGATCAAATAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGtt              <  2:367804/141‑1 (MQ=255)                                                                                                                                    aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat        <  1:74739/141‑1 (MQ=255)                                                                                                                                    aGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGatat        >  1:363879/1‑141 (MQ=255)                                                                                                                                        aTTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaa    >  1:58104/1‑141 (MQ=255)                                                                                                                                          tCAGCTCAATATCAAATAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGATATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa  <  2:53844/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                           TACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGC‑CGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAA  >  USA300TCH1516_ALE/111374‑111650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑