Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP000731	6,552	+TCTCCAGGCACTTGTGCATATTCTGAAA	100%	intergenic (+1069/‑419)	cadX → / → USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000731	= 6552	0 (0.000)	16 (0.620) +TCTCCAGGCACTTGTGCATATTCTGAAA	16/194	NT	100%	intergenic (+1069/‑419)	cadX/USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator
	?	CP000731	6553 =	0 (0.000)					intergenic (+1070/‑418)	cadX/USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator

AAAATTTCAGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000731/6418‑6552 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATCTCTAATTA  >  CP000731/6553‑6687                                                                                                                                        ||||||||||||||||||||||||||||                                                                                                                                        AAAATTTCAGTTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACT                                                                                                                                                                    <  2:100056/136‑1      TTCAGGTTGTGATTTGGTATGCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGTTGTGCCATATTCCATAGACTTTTTTATTGTCATTTTTTGGGGAATAGGAACAATCCACTCTCCA                                                                                                                                                               <  1:17713/136‑1        CAGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGG                                                                                                                                                             <  1:91647/136‑1                    TGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCTATATACCATAGACTTTTTAATAGATATTTTTTGGGGAATAGGATTAATCTACTTTCCAGGCACTTGTGCATA                                                                                                                                                 <  1:18709/136‑1                    TGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCATTCTCCAGGCACTTGTGCATA                                                                                                                                                 <  1:73127/136‑1                         TTCTTTCCATATCTTTCTAGTTCAAAAATTGATAATGCTGCCGGAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTTGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTG                                                                                                                                            <  2:40106/136‑1                         TTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGTGGAATAGGAATAATTCATTCTCCAGGCACTTGTGCATATTCTG                                                                                                                                            <  1:12515/136‑1                                    TTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGTGTAATAGGAACAATCCTCTCTCCAGGCATTTGTGCATATTCTGAAAATCCACCT                                                                                                                                 <  1:69010/136‑1                                       TTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATATACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGTTACTTGTGCATATTCTGAAAATCCACCTGTA                                                                                                                              <  1:60610/136‑1                                          TAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTTCATATTCTGAAAATCCACCTGTATGT                                                                                                                           <  1:94441/136‑1                                                     TTGATAATGCTGCCGTAAAACCAGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAAC                                                                                                                >  1:97276/1‑136                                                      TGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACA                                                                                                               <  2:24259/136‑1                                                        ATAATGCTGCCGTAAAAACGGCTATAACAAAAAACATAGACTAATTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGTCACTTGTGCATATTCTGAAAATCCACCTCTATGTGTCCCTCCAACATC                                                                                                             >  1:12286/1‑136                                                                           GGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATTATAACCAGTAACTAAGACA                                                                                          <  1:118911/136‑1                                                                                   CATATACCATAGACTCTTTAATGGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCC                                                                                  <  2:133731/136‑1                                                                                    ATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATTCATTATCGCCT                                                                                 <  2:76356/136‑1                                                                                          CATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAATTAATACATTATCGCCGTCTTTA                                                                           <  1:12727/136‑1                                                                                                 TCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTAT                                                                    >  1:41175/1‑136                                                                                                     TAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATT                                                                <  2:95985/136‑1                                                                                                       ATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCc                                                              >  2:64279/1‑135                                                                                                        TAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAATCAGTAACTAATACATTATAGACTTCATTAAAATTATCATTCGA                                                             >  2:11299/1‑136                                                                                                                     GGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTTTTACTTTATCGTTTTTTTTATACTTATTATTCGAACTTGATTCAACA                                                <  1:103238/136‑1                                                                                                                       tAATAGTAACAATCTTCTCTTCATGCACTTTTTCATATTCTGAAAATCCACCTGTATGTGTCACTCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTTATTTAACAAT                                              <  2:144885/135‑1                                                                                                                                           CCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATAACAG                          >  2:11258/1‑136                                                                                                                                            CAGGCACTTGTGCATATTCTGAAAATCCACCAGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGG                         >  1:123195/1‑136                                                                                                                                              GGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAA                       >  2:29982/1‑136                                                                                                                                                    TGTGCATATTCTGAAAATCCACCTGTATGTATCACGCCAACATAATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAG                 >  1:157241/1‑136                                                                                                                                                                   AATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATCTCTAATTA  >  1:18657/1‑136                                                                                                                                        ||||||||||||||||||||||||||||                                                                                                                                        AAAATTTCAGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000731/6418‑6552 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATCTCTAATTA  >  CP000731/6553‑6687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.