Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA100.0% 34.7 / NA 15A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/4);  total (11/4)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATT  >  USA300TCH1516_ALE/2279583‑2279829
                                                                                                                          |                                                                                                                            
ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGGTGTGTCGACACAAAAATTGTTATTAGCTTTCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAcc                                                                                                                 <  1:61001/136‑1 (MQ=255)
    ttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                             >  1:91098/1‑136 (MQ=255)
                        ttACTTCGTTATCCATTGTCGGAATATTTATTAATACATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTgg                                                                                         >  1:6256/1‑136 (MQ=255)
                                 tATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTACGGTTTATCATTTGCATCATCAGTTGATGATGTTACGGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg                                                                                >  1:161184/1‑136 (MQ=255)
                                 tATCCATTGTCGGACTGTTTGTTGATACAAATACACAAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg                                                                                >  1:118575/1‑136 (MQ=255)
                                      aTTGTCGGACTGTTTGTTGATGCATATACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGTATCATCAATTGCTGATGTTAATGTTGTTTCATTTGTTACCGCATAAATACTAAGTGGTGTGGTGGgagatg                                                                           >  2:121392/1‑134 (MQ=255)
                                                    tgttgCTGCATCTACACTAGAAATGATATTAACTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                             >  1:72024/1‑136 (MQ=255)
                                                                                ttaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTATTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTCGGtt                                 >  1:125867/1‑136 (MQ=255)
                                                                                ttaGCTTGCGGTTTATCATTTACATCATCAGTAGCAGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGtt                                 >  1:39541/1‑136 (MQ=255)
                                                                                     ttGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTTTTGCCGCATCCCTATTATTTGGTGTTGTCGGAGCAGCGTCTGCTTTGCCATTAGCTGTCGTCTAAGATCCGGTAGAttcttca                            >  2:94886/1‑131 (MQ=255)
                                                                                      tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCTGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTTCCATTAGCTGTCGTCTTAGATACGATAGGTTGTCCAg                           <  1:26084/136‑1 (MQ=255)
                                                                                        cGGTTTATCATTTGCATCATCAGTAGCTAATGTTAATGTAGATTCACATGTTGCCGCATAACTATTAATCACTATTATCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTa                         >  1:162448/1‑136 (MQ=255)
                                                                                                 aTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                <  2:91098/136‑1 (MQ=255)
                                                                                                          atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTa       <  2:6256/136‑1 (MQ=255)
                                                                                                               ttGCTGCTGTTACTGTTATTTCAAAAATTAACACATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAtt  >  1:120765/1‑136 (MQ=255)
                                                                                                                          |                                                                                                                            
TTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATT  >  USA300TCH1516_ALE/2279583‑2279829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑