| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 100.0% | 46.1 / NA | 19 | T15T (ACA→ACT) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (6/13); total (6/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACA > USA300TCH1516_ALE/2532167‑2532427 | tttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTActacaa < 2:121108/136‑1 (MQ=255) aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGTATGAGTTCAATTTTTTTACTATAAAGCATTTTTCACAATacac < 1:58116/136‑1 (MQ=255) aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc < 2:61080/136‑1 (MQ=255) attaATAAATTTAAGAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc < 2:129981/136‑1 (MQ=255) tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc < 2:21052/136‑1 (MQ=255) gagGGATAGGATAAAATACACTTTGTAACGTTGACTTCAGATGAGTTCAATGCGTTTACTACAAAGAATTTTTCACATTACACACAATCATCTATTCATTACAATCATATAGTTTATTTAAAAGGCGATGTGCATc < 2:44596/136‑1 (MQ=255) gatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGTTATTCATTATTATTTTAGTGTTTATTTAAAAGGCGATGTGCATCTTGt < 1:106779/136‑1 (MQ=255) tGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt > 1:25942/1‑136 (MQ=255) gTAACGTTGACTTCGGATGAATTAAATACAAAAACTACAAAACATAATTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAAGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATg > 1:6423/1‑136 (MQ=255) cGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa > 2:75663/1‑136 (MQ=255) tttaCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGTTGTGCATTTTTTTGGGGTTAAAGATGACAATGGTCAAg < 1:143090/133‑1 (MQ=255) tGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGt < 2:58567/136‑1 (MQ=255) gACTTCGGATGAGTTCAAGGTGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTg < 2:156990/136‑1 (MQ=255) cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAATGCGATGTGTATCTTGTATGGGTTAAAGATGACAATGGTCAAGTGAt < 1:51429/136‑1 (MQ=255) ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGTATCTTGTTGTGTTTAAAGATGACAATGGTCAAGTGAtt < 1:95087/136‑1 (MQ=255) ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGTATCTTGTAGGGTTTAAAGATGACAATGGTCAAGTGAtt < 1:157932/136‑1 (MQ=255) ttCGGATGAGTTCAATGCGTTTACTACAAAGCATATTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt > 1:149905/1‑136 (MQ=255) ttCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCACTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAAGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGACGCt > 2:122997/1‑136 (MQ=255) actGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTAGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt > 1:7106/3‑136 (MQ=255) gTTTATTACAAAGCATTTTTCAGATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACa < 2:141467/136‑1 (MQ=255) | TTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACA > USA300TCH1516_ALE/2532167‑2532427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |