Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 512,643 A→G 49.3% intergenic (+711/‑6052) recR → / → speA_2 Recombination protein RecR/Arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE512,6430AG49.3% ‑2.3 / 27.6 27intergenic (+711/‑6052)recR/speA_2Recombination protein RecR/Arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (10/3);  new base G (4/9);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 4.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGC  >  USA300TCH1516_ALE/512512‑512767
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ttttACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTtaataa                                                                                                                          >  1:65545/1‑136 (MQ=255)
taagctccttagcgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatgaacaagataaAAACAGTTATTGACTTCAATGTtgataa                                                                                                                          <  2:107935‑M1/28‑1 (MQ=255)
      ccttagcgtcgatagtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaataataaataaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGta                                                                                                                    >  2:80215‑M1/103‑136 (MQ=255)
            cgtcgatggtagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaattggacaagataaATAATGTTTTTGACTTAAATGTTGATAAAATGTAgaatta                                                                                                              >  1:57276‑M1/97‑136 (MQ=255)
                   tttGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTg                                                                                                       >  2:67031/1‑136 (MQ=255)
                   ttggAAAGTCTGTATAGTATTTATTTGTTGAGTCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTg                                                                                                       <  2:68651/133‑1 (MQ=255)
                     tagtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGATTTAAATGTTTATAAAATGTATAATTAATTCTTGTc                                                                                                     >  1:17805‑M1/88‑136 (MQ=255)
                      agtcgaacttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCg                                                                                                    >  1:71703‑M1/87‑136 (MQ=255)
                           ccTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAg                                                                                               >  2:48449/1‑136 (MQ=255)
                            acttacgttccgctagagtagaacgttgccaggcagttttttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTATTGTCGGTAAGa                                                                                              <  2:55822‑M1/56‑1 (MQ=255)
                                                         caggcagttttttaatcaaattgtgagtaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTAGATGACAat                                                                 <  2:50987‑M1/85‑1 (MQ=255)
                                                           aacaacTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAatat                                                               >  2:98869/1‑136 (MQ=255)
                                                               actcaactTAAGAAATAACTTGAATTACTAACGAAAATTAATCTTAAAAAGTTCTTGACTTAAATGTTAATAAAATCTCTAATTAATTATTGTACGTAACAAACATAACCATTGAAAACTGAATAACAATATGTCa                                                           >  2:35283/1‑136 (MQ=255)
                                                                 tcaactTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTACGAAAAATGAACATTGAAAACTGAATGACAATATGTCaaa                                                         >  2:113277/1‑135 (MQ=255)
                                                                   tttaatcaaattttggttaaaaaataaaatggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATTATTAATTCTTGTCGGTAAGAAAAATTAACATTGAAAACTGAATGACAATATGTCAACGt                                                       >  1:110461‑M1/42‑136 (MQ=255)
                                                                                    gAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTa                                      >  2:113104/1‑136 (MQ=255)
                                                                                     aaTTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTaa                                     <  2:65545/136‑1 (MQ=255)
                                                                                         aCTAACGAACATTAATTTTAAAAAAATATTGACTAAAAAAATAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACtat                                 >  1:43732/1‑136 (MQ=25)
                                                                                          cTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACAATGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACtata                                <  1:67031/136‑1 (MQ=255)
                                                                                               atggacaagataaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTa                           <  2:86140‑M1/123‑1 (MQ=255)
                                                                                                         taaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACattat                 <  2:131952‑M1/133‑1 (MQ=255)
                                                                                                         taaAAAAAGTTATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAAAGTTAATTACAAAAAACGTAAATATAAGTTACAAACattat                 <  2:127874‑M1/133‑1 (MQ=255)
                                                                                                          aagaataGTGATTGACTTAAATGTTGATAAAATGTATAATTAAGTGGTGTCGGTAATAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACattatt                <  2:41682/130‑1 (MQ=18)
                                                                                                                   tATTGACTTAAATGTTGATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCATAAATCTTAATTATAAGTTACAAACATTATTTAGTATTTa       <  1:31053/136‑1 (MQ=21)
                                                                                                                    aTTGTCTTAAATGTTAATAAAATATATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTAt      >  1:35242/1‑136 (MQ=255)
                                                                                                                    aTTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTAt      >  1:133016/1‑136 (MQ=255)
                                                                                                                      tGACTTAAATGTTAATAAAATGTATAATTAAAAATTGACAATAAGAAAAATAAAAAATAAAAAATAAATGACAATATGTCAAAGTTAATTCCAAAAAACGTAACTATAAGGTAAAAACAAAATTTAGGAATTAtaa    >  1:131037/1‑134 (MQ=12)
                                                                                                                       gACTTAAATGTTGATAAAATGTATAATTATATCTTGTCATCAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAg   <  1:52795/136‑1 (MQ=17)
                                                                                                                        aatgaaaTGTTGAAAAAATGAATAATTAATTCTTGTCGGTAAGAAAAATGAATATTGAAAACTGAATGACAATATGTCAAAATAAAAAAAAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGc  <  2:59756/132‑1 (MQ=18)
                                                                                                                                   |                                                                                                                            
TTTTACTGTTGTTAAGCAGTTTGAAAGCCTGTATAGTATTTATTTGTTGAGGCAAACAAAACAACTCAACTTAAGAAATAACTTGAATTACTAACGAAAATTAATTTTAAAAAGTTATTGACTTAAATGTTAATAAAATGTATAATTAATTCTTGTCGGTAAGAAAAATGAACATTGAAAACTGAATGACAATATGTCAACGTTAATTCCAAAAAACGTAACTATAAGTTACAAACATTATTTAGTATTTATGAGC  >  USA300TCH1516_ALE/512512‑512767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: