BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,710,898	(T)_6→7	coding (29/1812 nt)	oatA_2 ←	O‑acetyltransferase OatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,710,892	1	.	T	95.0%	441.1 / 16.2	142	coding (35/1812 nt)	oatA_2	O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand): ref base . (5/2); new base T (77/57); total (82/60)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 152 total aligned reads displayed.

ATAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑TTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCTTA  >  USA300TCH1516_ALE/2710753‑2711027

aTAACCTGAAAGAACGAAGAATGTATGTACTCCTAAAAAGCCCCCACGTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGCTAAGTATCGCGGGGAACA‑CA‑t                                                                                                                                         >  2:663580/1‑141 (MQ=255)

 tAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑tt                                                                                                                                        <  1:509681/141‑1 (MQ=255)

   aCCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑ttt‑t                                                                                                                                     >  2:8565/1‑141 (MQ=255)

    ccTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑ttt‑tt                                                                                                                                    <  1:497753/141‑1 (MQ=255)

      tGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑ttt‑tttt                                                                                                                                  <  2:16614/141‑2 (MQ=255)

       gAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATA‑CA‑TTT‑TTTCt                                                                                                                                 >  4:475774/1‑141 (MQ=255)

       gAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑ttt‑ttttc                                                                                                                                 >  1:1069505/1‑139 (MQ=255)

        aaaTAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑ttt‑ttttct                                                                                                                                >  2:419110/1‑138 (MQ=255)

          aTAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑ttt‑ttttctaa                                                                                                                              <  1:122406/141‑6 (MQ=255)

          aTAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑ttt‑ttttctaa                                                                                                                              <  1:513015/141‑6 (MQ=255)

             aCGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGTGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACg                                                                                                                            >  4:592630/1‑141 (MQ=255)

             aCGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACg                                                                                                                            >  1:1034803/1‑141 (MQ=255)

              cGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGt                                                                                                                           >  4:386142/1‑141 (MQ=255)

               gaagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAATGtt                                                                                                                          >  2:388101/1‑141 (MQ=255)

                aagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTACACGttt                                                                                                                         >  2:958640/1‑141 (MQ=255)

                aagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGttt                                                                                                                         >  1:271730/1‑141 (MQ=255)

                aagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGttt                                                                                                                         >  3:366366/1‑141 (MQ=255)

                 agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTa                                                                                                                        <  1:389692/141‑1 (MQ=255)

                 agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATGCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTa                                                                                                                        <  3:5312/141‑1 (MQ=255)

                     tGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGt                                                                                                                    <  2:869034/141‑1 (MQ=255)

                       tATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCt                                                                                                                  <  3:196674/141‑1 (MQ=255)

                        aTCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACCT‑TTT‑TTTCTAAACGTTTAAAGTCtt                                                                                                                 >  3:447697/1‑141 (MQ=255)

                         tCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCttt                                                                                                                <  4:10946/141‑1 (MQ=255)

                         tCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCttt                                                                                                                >  4:119274/1‑141 (MQ=255)

                                 tAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCa                                                                                                        >  1:875491/1‑141 (MQ=255)

                                   aaaaGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATg                                                                                                      >  2:725802/1‑141 (MQ=255)

                                     aaGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGtt                                                                                                    <  3:92011/141‑1 (MQ=255)

                                     aaGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGtt                                                                                                    >  4:301600/1‑141 (MQ=255)

                                     aaGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGtt                                                                                                    >  2:477139/1‑141 (MQ=255)

                                       gCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTtaa                                                                                                  <  2:287435/141‑1 (MQ=255)

                                       gCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTtaa                                                                                                  >  3:514212/1‑141 (MQ=255)

                                         ccccACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTtaata                                                                                                >  2:632957/1‑141 (MQ=255)

                                            cACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAAc                                                                                             >  4:190823/1‑141 (MQ=255)

                                              cTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGc                                                                                           >  1:960431/1‑141 (MQ=255)

                                                tAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGccc                                                                                         <  4:19594/141‑1 (MQ=255)

                                                 aaCCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGcccc                                                                                        >  1:588759/1‑141 (MQ=255)

                                                    cATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATc                                                                                                          >  3:24058/1‑120 (MQ=255)

                                                    cATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCatt                                                                                     >  2:1208611/1‑141 (MQ=255)

                                                             ttCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTTTTTATGTTTAAAGTTTTTTGTATCCATGTTAATAAACGCCCCATTTAttttttt                                                                            >  2:1138318/1‑139 (MQ=255)

                                                             ttCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtct                                                                            >  2:413887/1‑141 (MQ=255)

                                                             ttCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtct                                                                            >  2:1048124/1‑141 (MQ=255)

                                                              tCAAGTGATAAATAATGTTTTTTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTtctc                                                                           <  3:153580/141‑1 (MQ=255)

                                                                aaGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTa                                                                         <  2:557253/141‑1 (MQ=255)

                                                                  gTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAtt                                                                       >  1:953861/1‑141 (MQ=255)

                                                                  gTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAtt                                                                       >  2:501318/1‑141 (MQ=255)

                                                                   tGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTTTTTTTTTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTATCTAttt                                                                      >  2:755488/1‑141 (MQ=255)

                                                                   tGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTAttt                                                                      >  4:41601/1‑141 (MQ=255)

                                                                      taaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGt                                                                   >  2:304824/1‑141 (MQ=255)

                                                                      taaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGt                                                                   >  2:305802/1‑141 (MQ=255)

                                                                         ataatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTg                                                                                                              >  2:1227150/1‑95 (MQ=255)

                                                                         ataatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGc                                                                >  1:469108/1‑141 (MQ=255)

                                                                           aatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTg                                                                    <  4:296108/135‑1 (MQ=255)

                                                                              gATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATA‑CA‑TTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATca                                                          >  3:305498/1‑141 (MQ=255)

                                                                              gATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTAttttt                                                                              <  1:880061/122‑1 (MQ=255)

                                                                                  ccTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACaa                                                       >  2:760301/1‑141 (MQ=255)

                                                                                  ccTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACaa                                                       >  1:605462/1‑141 (MQ=255)

                                                                                  ccTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACaa                                                       <  4:178797/141‑1 (MQ=255)

                                                                                  ccTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACaa                                                       >  2:1339429/1‑141 (MQ=255)

                                                                                       aaCTGCGAATGCACTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATAttt                                                  >  4:240516/1‑141 (MQ=255)

                                                                                        aCTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATAtttt                                                 >  3:251704/1‑141 (MQ=255)

                                                                                            cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaa                                             >  1:269146/1‑141 (MQ=255)

                                                                                            cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaa                                             >  2:1047034/1‑141 (MQ=255)

                                                                                             gAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaa                                            >  1:232688/1‑141 (MQ=255)

                                                                                             gAATGCCCTCAATCCATCTAATCCAGGTAAGGGGCGGGGGTAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaa                                            >  2:62771/1‑141 (MQ=255)

                                                                                              aaTGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaa                                           <  2:661205/141‑1 (MQ=255)

                                                                                                   ccTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTAt                                                                                                           >  1:263785/1‑72 (MQ=255)

                                                                                                   ccTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTAt                                                                                                           <  2:263785/72‑1 (MQ=255)

                                                                                                       aaTCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATAtt                                  >  3:478013/1‑141 (MQ=255)

                                                                                                       aaTCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATAtt                                  >  3:418203/1‑141 (MQ=255)

                                                                                                        aTCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTg                                 >  4:17174/1‑141 (MQ=255)

                                                                                                           cATATAATCCAAGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCAAAAAAAAAAAAAAAATAAAATATTGCAc                              <  2:920233/141‑1 (MQ=255)

                                                                                                            aTCTACTCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTAGCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACt                             <  1:195943/141‑1 (MQ=255)

                                                                                                            aTCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACt                             <  2:996833/141‑1 (MQ=255)

                                                                                                            aTCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACt                             <  1:19371/141‑1 (MQ=255)

                                                                                                            aTCTAATACAGGTAAGTATCGCGGGGTATA‑CATTTT‑TTTCTAACCGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACt                             <  4:235265/141‑1 (MQ=255)

                                                                                                             tCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTg                            <  2:557016/141‑1 (MQ=255)

                                                                                                             tCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTg                            >  1:1235287/1‑141 (MQ=255)

                                                                                                             tCTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTg                            >  1:501896/1‑141 (MQ=255)

                                                                                                              cTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTa                                                                                                                        >  1:157414/1‑48 (MQ=255)

                                                                                                              cTAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTa                                                                                                                        <  2:157414/48‑1 (MQ=255)

                                                                                                               tAATCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAt                          <  1:443265/141‑1 (MQ=255)

                                                                                                                aaTCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAtt                         >  1:146357/1‑141 (MQ=255)

                                                                                                                aaTCCAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAtt                         <  3:190823/141‑1 (MQ=255)

                                                                                                                 gtCCAGGTAAGTATCGCGGGGACTA‑CAGTTT‑TTTCTAAACGTTTAAGGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATACTTTTGAAAATAAAATATTGCACTGAttt                        <  2:881550/140‑1 (MQ=255)

                                                                                                                   ccAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAATGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTc                      <  1:388101/141‑1 (MQ=255)

                                                                                                                    cAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCa                     <  3:476698/141‑1 (MQ=255)

                                                                                                                    cAGGTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCa                     <  2:777618/141‑1 (MQ=255)

                                                                                                                      ggTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTTAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCAtt                   >  2:1253615/1‑141 (MQ=255)

                                                                                                                      ggTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCAtt                   >  1:1346614/1‑141 (MQ=255)

                                                                                                                      ggTAAGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCAtt                   <  1:1344010/141‑1 (MQ=255)

                                                                                                                         aaGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAAt                <  2:1377288/141‑1 (MQ=255)

                                                                                                                          aGTATCGCGGGGAATA‑CATTTT‑TTTCTAAACTTTTAAATCTTTTTGTATTCTTCTTTTTATTCGCCTCTTTTATTGTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAAtt               <  1:1243247/141‑1 (MQ=255)

                                                                                                                           gTATCGCGGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTg              <  2:1383119/141‑1 (MQ=255)

                                                                                                                               cgcgGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGAttt          <  3:592630/141‑1 (MQ=255)

                                                                                                                                gcgGGGAATA‑CATTTT‑TTTCTTAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTa         >  2:1384318/1‑141 (MQ=255)

                                                                                                                                gcgGGGAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTa         <  3:17285/141‑1 (MQ=255)

                                                                                                                                   gggAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTAGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAAcc      <  1:1192483/141‑1 (MQ=255)

                                                                                                                                    gtaaTA‑CATTTT‑TTTCTAAACGTTGAAAGTCTTTTGTATCCATTTTAATAGACGCCCCATTTATTTTTGTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAGccc     <  4:514212/139‑1 (MQ=255)

                                                                                                                                    ggAATA‑CATTTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCAATAATTGATTTAAccc     >  3:359124/1‑141 (MQ=255)

                                                                                                                                       aTA‑CA‑TTTCTTTCTAAACGTTTAAAATCATTTGTATCCATGTTAATACACGCCCCATTTATTCTTCTATATTTTGTAGCATCTCAAAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCtta  <  1:697874/141‑1 (MQ=255)

ATAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATA‑CA‑TTT‑TTTCTAAACGTTTAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCTTA  >  USA300TCH1516_ALE/2710753‑2711027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`