BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,710,898	(T)_6→7	coding (29/1812 nt)	oatA_2 ←	O‑acetyltransferase OatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,710,892	1	.	T	93.8%	294.4 / 13.3	96	coding (35/1812 nt)	oatA_2	O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand): ref base . (2/4); new base T (55/35); total (57/39)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AATAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCTTAATTAA  >  USA300TCH1516_ALE/2710752‑2711032

aaTAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACa                                                                                                                                                 >  2:250555/1‑141 (MQ=255)

   aaCCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑ttt                                                                                                                                             <  4:319224/141‑1 (MQ=255)

     ccTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑ttttt                                                                                                                                           >  2:648729/1‑141 (MQ=255)

       tGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑ttttttt                                                                                                                                         <  2:657390/141‑2 (MQ=255)

          aaTAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACA‑TTTTTTCTaa                                                                                                                                      <  1:838323/141‑1 (MQ=255)

           aTAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑tttttttctaa                                                                                                                                     <  2:225570/141‑6 (MQ=255)

               cGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGt                                                                                                                                  >  1:884522/1‑141 (MQ=255)

               cGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGt                                                                                                                                  >  2:266700/1‑141 (MQ=255)

                gaagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTATTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGtt                                                                                                                                 >  2:968099/1‑141 (MQ=255)

                 aagaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTT‑a                                                                                                                              >  3:68309/1‑141 (MQ=255)

                  agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTT‑aa                                                                                                                             <  1:691653/141‑1 (MQ=255)

                  agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTGAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑a                                                                                                                              <  3:76137/141‑1 (MQ=255)

                  agaaTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑a                                                                                                                              <  2:441049/141‑1 (MQ=255)

                     aTGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAg                                                                                                                           <  1:195446/141‑1 (MQ=255)

                         aTCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCtt                                                                                                                       >  1:633187/1‑141 (MQ=255)

                          tCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCttt                                                                                                                      <  3:113634/141‑1 (MQ=255)

                            tACTCCTAAGAAGCCCCCGCGGAACCAGTGGTCATTCAAGTGATAAATAAGGAGTCCTAGAACTGCGAATGCCCTCAATCCATCTAATCCCGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTg                                                                                                                    <  1:461337/141‑1 (MQ=255)

                            tACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTg                                                                                                                    <  1:968099/141‑1 (MQ=255)

                               tCCTAAANAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTAt                                                                                                                 <  2:759140/141‑1 (MQ=255)

                               tCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTAt                                                                                                                 >  1:231554/1‑141 (MQ=255)

                               tCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATGCCTAGAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTAt                                                                                                                 <  1:461296/141‑1 (MQ=255)

                                ccTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATc                                                                                                                <  1:562344/141‑1 (MQ=255)

                                      aaGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGtt                                                                                                          <  1:955063/141‑1 (MQ=255)

                                        gCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTtaa                                                                                                        >  2:340363/1‑141 (MQ=255)

                                        gCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTtaa                                                                                                        >  2:84901/1‑141 (MQ=255)

                                        gCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTtaa                                                                                                        <  2:70822/141‑1 (MQ=255)

                                          ccccACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGttggaa                                                                                                      >  1:347532/1‑137 (MQ=255)

                                           cccACTGAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTtaataa                                                                                                     >  2:876136/1‑141 (MQ=255)

                                            ccACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGAAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATaaa                                                                                                    >  4:376592/1‑141 (MQ=255)

                                                 tAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGtt                                                                                                          <  1:500789/130‑1 (MQ=255)

                                                 tAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGtt                                                                                                          >  2:500789/1‑130 (MQ=255)

                                                 tAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGccc                                                                                               >  1:486160/1‑141 (MQ=255)

                                                   aCCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCa                                                                                             >  1:561212/1‑141 (MQ=255)

                                                     cATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCa‑tt                                                                                          >  1:841774/1‑141 (MQ=255)

                                                             aTTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTtc                                                                                  <  1:876136/141‑1 (MQ=255)

                                                             aTTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTtc                                                                                  >  4:21079/1‑141 (MQ=255)

                                                               tCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTtctc                                                                                <  2:784098/141‑1 (MQ=255)

                                                               tCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTtctc                                                                                <  3:183190/141‑1 (MQ=255)

                                                                   gTGATAAATAATGATTCCAATAACTGCGAATGCCCTCACTCCATCTACTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCGAAACGTTT‑ACAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTAtt                                                                            >  4:262957/1‑141 (MQ=255)

                                                                      ataaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCGGGTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGt                                                                        <  2:192604/141‑1 (MQ=255)

                                                                      ataaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTg                                                                         <  2:479527/141‑1 (MQ=255)

                                                                      ataaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTg                                                                         >  1:1005519/1‑141 (MQ=255)

                                                                       taaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTAt                                                                                                                 <  4:398177/101‑1 (MQ=255)

                                                                       taaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTAt                                                                                                                 >  3:398177/1‑101 (MQ=255)

                                                                       taaataaTGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGt                                                                        >  1:306562/1‑141 (MQ=255)

                                                                          ataatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAGTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGc                                                                     >  3:419042/1‑141 (MQ=255)

                                                                            aatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCat                                                                   >  2:758557/1‑141 (MQ=255)

                                                                            aatGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCat                                                                   >  1:738856/1‑141 (MQ=255)

                                                                             atGATTCCTATAACTGCGAATGCCCTCAAGCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCata                                                                  >  1:24280/1‑141 (MQ=255)

                                                                                aTTCCTATAACTGCGAATGCCCTCAATTCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATca                                                               >  4:275419/1‑141 (MQ=255)

                                                                                aTTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATca                                                               <  1:161723/141‑1 (MQ=255)

                                                                                aTTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATca                                                               <  1:648729/141‑1 (MQ=255)

                                                                                    cTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAat                                                           >  2:356235/1‑141 (MQ=255)

                                                                                    cTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAat                                                           >  2:79954/1‑141 (MQ=255)

                                                                                     tataACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAata                                                          <  2:115861/141‑1 (MQ=255)

                                                                                     tataACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAata                                                          <  2:70364/141‑1 (MQ=255)

                                                                                       taACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATAtt                                                        >  3:69272/1‑141 (MQ=255)

                                                                                        aaCTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATAttt                                                       >  2:131166/1‑141 (MQ=255)

                                                                                         aCTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATAtttt                                                      <  1:688515/141‑1 (MQ=255)

                                                                                             cGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAATGTTT‑AAAGTCATTTGTATCCATGTTAATAAACGCCCAA‑TTTATTTTTCTCTATTTTATAGAATTTCACAATATTTTTGaa                                                  >  4:103013/1‑141 (MQ=255)

                                                                                              gAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaa                                                 >  1:362134/1‑141 (MQ=255)

                                                                                               aaTGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaa                                                >  1:313604/1‑141 (MQ=255)

                                                                                                aTGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaat                                               <  2:48994/141‑1 (MQ=255)

                                                                                                aTGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaat                                               >  1:989854/1‑141 (MQ=255)

                                                                                                 tGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGaaaata                                              >  1:187905/1‑141 (MQ=255)

                                                                                                        aaTCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATAtt                                       >  2:281522/1‑141 (MQ=255)

                                                                                                        aaTCCATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATAtt                                       >  2:281517/1‑141 (MQ=255)

                                                                                                           ccATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCa                                    <  4:381811/141‑1 (MQ=255)

                                                                                                            cATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCAc                                   <  1:250555/141‑1 (MQ=255)

                                                                                                            cATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCAc                                   <  1:415696/141‑1 (MQ=255)

                                                                                                            cATCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCAc                                   >  1:177629/1‑141 (MQ=255)

                                                                                                             ttCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACt                                  <  2:835346/140‑1 (MQ=255)

                                                                                                             aTCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACt                                  <  2:513983/141‑1 (MQ=255)

                                                                                                             aTCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TGTATTTTTCTCTATTTTGTAGCATATCACAATATCTTTGAAAATAAAATATTGCACt                                  <  4:429569/141‑1 (MQ=255)

                                                                                                              tCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAACTAAAATATTGCACTg                                 >  4:418007/1‑141 (MQ=255)

                                                                                                              tCTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATAACAATATTTTTGAAAATCCAATATTGGACTg                                 >  1:614343/1‑141 (MQ=255)

                                                                                                               cTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGa                                >  2:950004/1‑141 (MQ=255)

                                                                                                               cTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGa                                >  2:334898/1‑141 (MQ=255)

                                                                                                               cTAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGAATATCACAATATTTTTGAAAATAAAATATTGctttga                                >  3:200462/1‑136 (MQ=255)

                                                                                                                tAATCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAt                               >  3:381839/1‑141 (MQ=255)

                                                                                                                 aaTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTTTTTTTTTAATATTGCACTGAtt                              >  4:149851/1‑141 (MQ=255)

                                                                                                                 aaTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAtt                              <  2:274962/141‑1 (MQ=255)

                                                                                                                 aaTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAtt                              >  2:535569/1‑141 (MQ=255)

                                                                                                                 aaTCCAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGAtt                              >  2:855966/1‑141 (MQ=255)

                                                                                                                    ccgggTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCa                          <  1:59045/138‑1 (MQ=255)

                                                                                                                    ccAGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTc                           <  1:612946/141‑1 (MQ=255)

                                                                                                                      aGGTAAGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCAt                         >  1:293234/1‑141 (MQ=255)

                                                                                                                          aaGTATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAAt                     >  3:121015/1‑141 (MQ=255)

                                                                                                                            gTATCGCGGGGAATACATTTTTTTCTAAACGTTTAAAAGTCTTTTGTATCCATGTTAATAAACGCCCCATTTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAAt                     <  2:772921/141‑1 (MQ=255)

                                                                                                                             tATCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGa                  <  1:311598/141‑1 (MQ=255)

                                                                                                                               tCGCGGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGAtt                >  2:1010910/1‑141 (MQ=255)

                                                                                                                               tCGCGGGGAATACA‑TTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGAttt               >  2:918618/1‑141 (MQ=255)

                                                                                                                                cgcgGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTAATTAATTGAttt               <  1:356235/141‑1 (MQ=255)

                                                                                                                                 gcgGGGAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAAAGCCCCA‑TTTATTATTTACTATTTGGTAGCATAGCACAATATTTTTGAATATAAAATATTGCACTGATTTTCATTAATTGATTTa              >  3:237122/1‑141 (MQ=255)

                                                                                                                                     ggAATACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAAccc          <  1:950004/141‑1 (MQ=255)

                                                                                                                                        aTACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCtta       >  2:1008464/1‑141 (MQ=255)

                                                                                                                                        aTACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCtta       >  2:877729/1‑141 (MQ=255)

                                                                                                                                        aTACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCtta       >  3:171290/1‑141 (MQ=255)

                                                                                                                                        aTACATTTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCtta       >  2:877737/1‑141 (MQ=255)

                                                                                                                                            a‑TTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCttaattaa  <  1:474102/141‑1 (MQ=255)

AATAACCTGAAATAACGAAGAATGTATCTACTCCTAAAAAGCCCCCACTTAACCATTGTGCATTCAAGTGATAAATAATGATTCCTATAACTGCGAATGCCCTCAATCCATCTAATCCAGGTAAGTATCGCGGGGAATACA‑TTTTTTCTAAACGTTT‑AAAGTCTTTTGTATCCATGTTAATAAACGCCCCA‑TTTATTTTTCTCTATTTTGTAGCATATCACAATATTTTTGAAAATAAAATATTGCACTGATTTTCATTAATTGATTTAACCCTTAATTAA  >  USA300TCH1516_ALE/2710752‑2711032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`