Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	829,184	G→A	14.9%	V246I (GTT→ATT)	comFA →	ComF operon protein 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	829,184	0	G	A	14.9%	150.0 / 15.5	66	V246I (GTT→ATT)	comFA	ComF operon protein 1
Reads supporting (aligned to +/- strand):  ref base G (30/25);  new base A (5/5);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCTAAATATCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAATATTAGAACGTGGATTTACAA  >  USA300TCH1516_ALE/829045‑829319                                                                                                                                            |                                                                                                                                        tCCTAAATATCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCgg                                                                                                                                         >  2:136857/1‑140 (MQ=255)  ccTAAATATCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGt                                                                                                                                        >  1:29612/1‑140 (MQ=255)   cTAAATATCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGtt                                                                                                                                       <  2:727021/140‑1 (MQ=255)      aaTATCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTtat                                                                                                                                    >  2:529793/1‑140 (MQ=255)        tatCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATATGTGGTTTATaa                                                                                                                                  >  2:58814/1‑140 (MQ=255)          tCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAACTGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGATTTATAAGc                                                                                                                                <  2:602675/140‑1 (MQ=255)          gccttctttCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGc                                                                                                                                <  1:541597/134‑1 (MQ=255)            gTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAg                                                                                                                              <  2:336994/140‑1 (MQ=255)            gTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAg                                                                                                                              <  1:418116/140‑1 (MQ=255)            gTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTATTGCTGTTAAACTAATACTCTCTGTTACACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAg                                                                                                                              <  2:360695/140‑1 (MQ=255)             ttATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAAAAACAATGATTAAAACATTTTCGGTTTATAAGCata                                                                                                                             >  2:102403/1‑138 (MQ=255)                tttCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGaaaa                                                                                                                          <  1:53241/140‑1 (MQ=255)                tttCAAACTTAATAACAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGaaaa                                                                                                                          <  2:467307/140‑1 (MQ=255)                 gtCAAACTTAAGAAGAAGAATATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTGTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAAt                                                                                                                         <  2:221080/139‑1 (MQ=255)                    aaaGTTTATAATAGGAATATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTTCATCTTCTTCTTTTTATAAGCAGAAAATTAc                                                                                                                      <  2:787296/140‑1 (MQ=255)                    aaaCTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTAc                                                                                                                      <  2:24809/140‑1 (MQ=255)                    aaaCTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTAc                                                                                                                      <  1:466441/140‑1 (MQ=255)                    aaaCTTAATAACAAGAATATTCAGAAAATGTTATACCTAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTAc                                                                                                                      <  2:309175/140‑1 (MQ=255)                     aaCTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACt                                                                                                                     <  2:715488/140‑1 (MQ=255)                         taataataaGAAGATTCAGAAAATGTTAGACCGAATGTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAAt                                                                                                                 >  1:595680/1‑140 (MQ=255)                          aataataaGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGATTTATAAGTAGAAAATTACTAAAtt                                                                                                                >  2:362567/1‑140 (MQ=255)                             aataaGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAAc                                                                                                             >  2:687226/1‑140 (MQ=255)                                 agaagaTTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATAc                                                                                                         >  1:713930/1‑140 (MQ=255)                                     gaTTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACATcc                                                                                                     >  2:223898/1‑140 (MQ=255)                                     gaTTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGAGTAAAACATTTTCGATTTATAAGCACAACATTAATAAATTAACATACGTcc                                                                                                     >  1:599645/1‑140 (MQ=255)                                        tCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATa                                                                                                  >  2:305315/1‑140 (MQ=255)                                         cAGAAAATGTTATACCGAATTTTATAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAg                                                                                                 <  2:788653/140‑1 (MQ=255)                                         cAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAg                                                                                                 >  2:411534/1‑140 (MQ=255)                                           gAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATGAGCAGAAAATTACTAAATTAACATACGTCCATAGCg                                                                                               >  1:329833/1‑140 (MQ=255)                                                 gTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATg                                                                                         >  1:427560/1‑140 (MQ=255)                                                  ttATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGt                                                                                        >  2:471236/1‑140 (MQ=255)                                                  ttATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGt                                                                                        >  2:261063/1‑140 (MQ=255)                                                  ttATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGt                                                                                        <  1:356119/140‑1 (MQ=255)                                                  ttATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGAGTTTTTTAACAATATAGAAACAATCATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGt                                                                                        >  2:403641/1‑140 (MQ=255)                                                   tataCCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTCAATTAACATACGTCCATAGCGAGGATGtt                                                                                       <  1:765041/140‑1 (MQ=255)                                                   tataCCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGtt                                                                                       <  1:685533/140‑1 (MQ=255)                                                   tataCCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCACAGCGAGGATGtt                                                                                       >  1:455719/1‑140 (MQ=255)                                                   tataCCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGATTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGtt                                                                                       <  2:633458/140‑1 (MQ=255)                                                   tataCCGAATTCTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGATTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGtt                                                                                       <  2:295350/140‑1 (MQ=255)                                                     taCCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGtttt                                                                                     >  1:329553/1‑140 (MQ=255)                                                                aCAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATATGTCAATAGCGAGGATGTTTTTCGCTTTGaaa                                                                          >  1:144406/1‑140 (MQ=255)                                                                aCAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGcaa                                                                          >  1:220555/1‑140 (MQ=255)                                                                      tCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGAGGTTTTTCGCTTTGAAAAAGTTg                                                                    >  2:216214/1‑140 (MQ=255)                                                                                          ttaCACTGGTGTTTTTTAACAAAATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACAt                                                <  2:472616/139‑1 (MQ=255)                                                                                             cacTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTc                                             >  1:634983/1‑140 (MQ=255)                                                                                             cacTAGTGTTTTTTAACAATATAGAAACAATAATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTc                                             <  2:213287/140‑1 (MQ=255)                                                                                                tGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGAt                                          <  1:687226/140‑1 (MQ=255)                                                                                                  ttgtTTTTTACAAATACAGAAACAATGACAAAAACATTTTCGATTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAATGAATGGACATTTCGATGt                                        <  2:78566/139‑1 (MQ=255)                                                                                                   tgtTTTTTAACAATATAAAAAAACAGATTAAAACATTTTCGATTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTc                                       <  2:599645/140‑1 (MQ=255)                                                                                                    gtTTTTTAACAATATAGAAACAATGAAAAAAAGATGTTGTGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCa                                      >  1:554077/1‑140 (MQ=255)                                                                                                    gtTTTTTAAAAATATAGAAACAATGATTAAAAGATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCa                                      <  2:67392/140‑1 (MQ=255)                                                                                                     ttttttAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCAt                                     >  1:56032/1‑140 (MQ=255)                                                                                                      tttttAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCAtt                                    >  2:346758/1‑140 (MQ=255)                                                                                                       ttttAACAATATAGAAACAATGATTAAAACATTTTCGATTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCAttt                                   >  1:50684/1‑140 (MQ=255)                                                                                                          tAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTta                                <  2:552235/140‑1 (MQ=255)                                                                                                            aCAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGCCATTTTtact                              >  1:707597/1‑140 (MQ=255)                                                                                                             cAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTtacta                             <  2:713930/140‑1 (MQ=255)                                                                                                                    gAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAat                      >  1:36523/1‑140 (MQ=255)                                                                                                                      aaCAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAatat                    <  1:529793/140‑1 (MQ=255)                                                                                                                      aaCAATGATTAAAACATTGTCGGTTTATAAGCAGAAAATTACGAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAatat                    <  1:799647/140‑1 (MQ=255)                                                                                                                        cAATTATTAAAACATTTTAGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAATATTa                  <  2:403877/140‑1 (MQ=255)                                                                                                                         aaTGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGGCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTAATTTTTACTACGACAATATTAg                 >  2:80002/1‑140 (MQ=255)                                                                                                                             aTTAAAACATTTTCGATTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAATATTAGAACg             >  2:623557/1‑140 (MQ=255)                                                                                                                                      ttttCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTTATTTTTACTACGACAATATTAGAACGTGGATTTAc    >  1:545390/1‑140 (MQ=255)                                                                                                                                      ttttCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAATATTAGAACGTGGATTTAc    >  1:544436/1‑140 (MQ=255)                                                                                                                                       tttCGATTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGAAAATATTAGAACGTGGATTTACa   >  2:429541/1‑140 (MQ=255)                                                                                                                                        ttCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAATATTAGAACGTGGATTTCCaa  >  2:432766/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        TCCTAAATATCGTTATTTCAAACTTAATAATAAGAAGATTCAGAAAATGTTATACCGAATTTTACAAGATCAAATTAATAATCAACGTTATACACTGGTGTTTTTTAACAATATAGAAACAATGATTAAAACATTTTCGGTTTATAAGCAGAAAATTACTAAATTAACATACGTCCATAGCGAGGATGTTTTTCGCTTTGAAAAAGTTGAACAATTAAGGAATGGACATTTCGATGTCATTTTTACTACGACAATATTAGAACGTGGATTTACAA  >  USA300TCH1516_ALE/829045‑829319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑