Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,402,143 G→T 7.8% A175D (GCT→GAT)  tyrA ← Prephenate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,402,1430GT 7.8% 226.4 / 2.4 81A175D (GCT→GAT) tyrAPrephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (39/35);  new base T (4/3);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATATCACGAAAACCACCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAGTGCTTTTTAGCATTTAG  >  USA300TCH1516_ALE/1402004‑1402276
                                                                                                                                           |                                                                                                                                     
gATATCACGAAAACCACCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTcag                                                                                                                                       <  1:683472/140‑1 (MQ=255)
 atatCACGAAAACCACCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTcagc                                                                                                                                      <  1:453987/140‑1 (MQ=255)
 atatCACGAAAACCACCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTcagc                                                                                                                                      <  1:37270/140‑1 (MQ=255)
     cACGAAAACCACCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGATCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGTGGAACATGACTTACGCCGCTTGCTACGTACTAGCGTTCTTCAGCagta                                                                                                                                  >  2:126746/1‑140 (MQ=255)
       cGAAAACCCCCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGACGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCagtagt                                                                                                                                <  2:652190/140‑1 (MQ=255)
           gaccaccAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACa                                                                                                                            <  2:378456/139‑1 (MQ=255)
           aaCCCCCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACa                                                                                                                            <  2:378459/140‑1 (MQ=255)
           aaCCACCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTATATGACTTACGTCGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACa                                                                                                                            >  1:456695/1‑140 (MQ=255)
            accaccAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACaa                                                                                                                           <  1:633881/140‑1 (MQ=255)
             ccaccaGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAATTGACTTACGTCGCTTGTTACGTAGTCGTGTTCTTCATCAGTAGTTACAAt                                                                                                                          >  1:128687/1‑140 (MQ=255)
             ccaccaGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCATCAGTAGTTACAAt                                                                                                                          >  1:200994/1‑140 (MQ=255)
             ccaccaGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCATCAGTAGTTACAAt                                                                                                                          >  1:607884/1‑140 (MQ=255)
                 caGCTGGAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAAt                                                                                                                      <  2:133430/140‑1 (MQ=255)
                 caGCTGCAGGTGTATTAACTAAATGATGTGCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAAt                                                                                                                      <  2:310999/140‑1 (MQ=255)
                 caGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTCGTCGTGTTCTTGAGCAGTGGTTACAAtgtat                                                                                                                      >  1:356586/1‑136 (MQ=255)
                 caGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAAt                                                                                                                      >  2:490135/1‑140 (MQ=255)
                 caGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAAt                                                                                                                      <  1:789325/140‑1 (MQ=255)
                 caGCTGCAAGTTTATTAACTAAAGGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAAt                                                                                                                      <  2:680842/140‑1 (MQ=255)
                   gCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAAttt                                                                                                                    >  2:76096/1‑140 (MQ=255)
                        aaGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCaa                                                                                                               <  2:443023/140‑1 (MQ=255)
                          gTTTATTAACTAAATGATGTTCTTCACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAaga                                                                                                             <  1:243563/140‑1 (MQ=255)
                              attaACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGCAGTTACAATAAATTGACCAagagggg                                                                                                         >  1:455791/1‑137 (MQ=255)
                               ttaACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGCCGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAgg                                                                                                        >  2:91373/1‑140 (MQ=255)
                               ttaACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTCGTTACAATAAATTGAGCAAGGGTAgc                                                                                                        >  2:91372/1‑139 (MQ=255)
                                 aaCTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATAAGAGGTAAATGACTTACGACGCTTGTTACGTAGGCGTGTTCTTCTGCCGTCGTTACAATAACTTTAGCATGAGGAGGTg                                                                                                      >  1:25888/1‑140 (MQ=255)
                                    tAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTTCTCTGCAGTTGTCACACTCAATTTTGCCAGAGTAGTAGATa                                                                                                   >  1:630304/1‑140 (MQ=255)
                                    tAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACATTATATTTAGCAAGAGTAGGTGTTa                                                                                                   >  1:177382/1‑140 (MQ=255)
                                    tAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGGGTAGGTGATa                                                                                                   >  2:518626/1‑140 (MQ=255)
                                     aaaTGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATaa                                                                                                  <  1:754288/140‑1 (MQ=255)
                                          atgTTCTTGACCGTTCTTTTGACTAACATGAACTAAAATAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGtt                                                                                             >  2:120169/1‑140 (MQ=255)
                                            gTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAACTGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTTCCATACATTTAGCAAGAGTAGGTGATAACAGTTCt                                                                                           >  1:119880/1‑140 (MQ=255)
                                               cTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATATATTTAGCAAGTGTAGGTGATAAGAGTACtttt                                                                                        >  1:1154/1‑140 (MQ=255)
                                                 tGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACGATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTGTaa                                                                                      >  1:138556/1‑140 (MQ=255)
                                                     cGTTCTTTTGAGTAACATGAAATATAAATGATTCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTGGTTACAATCAATTTAGGAAGAGGAGGTGGGAACAGGTGTGTTAGCgtg                                                                                  >  2:447083/1‑140 (MQ=255)
                                                     cGTTCTTTTGACTAACATGAATTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTTGTGTTCTTCAGTAGTAGTTACAATATATTTAGGAGGAGTAGGAGGTAACAGTTATTTGAACgtg                                                                                  >  2:448036/1‑140 (MQ=255)
                                                         cgtttGACTAACATGAACTAAGCTAGGGGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTATTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTtg                                                                              <  2:733293/138‑1 (MQ=255)
                                                          ttttGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTtgc                                                                             <  1:737258/140‑1 (MQ=255)
                                                           tttGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGTAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTTTTtgct                                                                            >  1:642459/1‑140 (MQ=255)
                                                           tttGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTCCAATAACTTTAGCCAGAGTAGGTGATAGCAGTTCTTTTACCGTGTTtgct                                                                            >  2:44260/1‑140 (MQ=255)
                                                           tttGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGCTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTtgct                                                                            <  1:802062/140‑1 (MQ=255)
                                                           tgtGACTTACGTGGACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTtgct                                                                            <  2:547829/138‑1 (MQ=255)
                                                           tgtGACTAACATGAACTAAACGAGATGCGACGATATGAGGTGGGTGACTAACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTtgct                                                                            <  2:778858/138‑1 (MQ=255)
                                                            ttGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTACTTCATCAGTAGTTACAATTAATTTAGCAAGAGTAGGGGGTCACAGTTCTTTTAACTTGTTtgctg                                                                           >  1:86377/1‑140 (MQ=255)
                                                              gACTAACATGAACTAAACTAGATACAACGATATGAGGTAAATCACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTgctgct                                                                         >  2:425110/1‑140 (MQ=255)
                                                                  aaCATTAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATATCAGTTCTTTTAACGTGTTTGCtgcttgct                                                                     <  1:349529/140‑1 (MQ=255)
                                                                  aaCATGAACTAAACTAGATGCTGTGCTTAGAGCTAAATGTGTTTTGGCTCTGTGAACCTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCtgcttgct                                                                     <  1:181478/140‑1 (MQ=255)
                                                                      tGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCAtt                                                                 <  2:536644/140‑1 (MQ=255)
                                                                       gAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCAttt                                                                <  1:62126/140‑1 (MQ=255)
                                                                       gAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCAttt                                                                <  1:777323/140‑1 (MQ=255)
                                                                       gAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGCTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCAttt                                                                >  2:682158/1‑140 (MQ=255)
                                                                          ctaaactaGATGCAACGATATGAGGTAAATGACTTACAACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTATGTGATAACAGTTCTTTTACCGTGTTTGATGCTTGCTCATTTCtt                                                             >  2:262986/1‑140 (MQ=255)
                                                                             aactaGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTGACGTGTTTGCTGCTTGCTAATTTATTGGc                                                          >  1:526928/1‑140 (MQ=255)
                                                                             aactaGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGc                                                          >  2:622790/1‑140 (MQ=255)
                                                                             aactaGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTAGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGTTAACAGTTCTTTTACCGGGTTTGCTGCTTGCTCATTTCTTGGc                                                          >  1:457270/1‑140 (MQ=255)
                                                                               ctaGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTc                                                        <  1:76096/140‑1 (MQ=255)
                                                                                    tGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGt                                                   >  1:724828/1‑140 (MQ=255)
                                                                                     gCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACGGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTa                                                  >  1:38006/1‑140 (MQ=255)
                                                                                     gCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTa                                                  <  1:747643/140‑1 (MQ=255)
                                                                                     gCAACGATACGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTa                                                  <  1:24072/140‑1 (MQ=255)
                                                                                      ccaCGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAg                                                 <  1:213139/138‑1 (MQ=255)
                                                                                      cAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAg                                                 >  1:689320/1‑140 (MQ=255)
                                                                                       agcGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGa                                                <  2:136226/138‑1 (MQ=255)
                                                                                        aCGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTGGAc                                               >  1:666340/1‑140 (MQ=255)
                                                                                        aCGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTGCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCCGCATGATTAGTTCTTGGCTAAGTGTAGAc                                               >  2:129744/1‑140 (MQ=255)
                                                                                         cGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACt                                              <  2:496682/140‑1 (MQ=255)
                                                                                              tGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAat                                         >  1:365086/1‑140 (MQ=255)
                                                                                                  gTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATataa                                     <  1:690822/140‑1 (MQ=255)
                                                                                                  gTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAAGataa                                     >  1:254566/1‑140 (MQ=255)
                                                                                                      aTGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGCAGGTGATCACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTCTAGACTAAAATATCATAAg                                 >  2:609406/1‑140 (MQ=255)
                                                                                                           ttACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGttt                            <  2:177474/140‑1 (MQ=255)
                                                                                                            tACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGtttt                           <  2:112725/140‑1 (MQ=255)
                                                                                                              cgacgCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCa                         >  2:493109/1‑140 (MQ=255)
                                                                                                              cgacgCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGCAGACTAAAATATAATAAGCGTTTTCa                         >  1:724212/1‑140 (MQ=255)
                                                                                                               ggcgCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAGCAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCaa                        <  2:400949/138‑1 (MQ=255)
                                                                                                                    ttGTTACGTAGTCGTGTTCTTCATCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAg                   <  2:200994/140‑1 (MQ=255)
                                                                                                                    ttGTTACGTAGTCGTGTTCTTCATCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAg                   <  2:128687/140‑1 (MQ=255)
                                                                                                                    ttGTTACGTAGTCGTGTTCTTCATCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTATGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAg                   <  2:607884/140‑1 (MQ=255)
                                                                                                                    ttGTTACGTAGTCGTGTTCCTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAg                   <  1:645180/140‑1 (MQ=255)
                                                                                                                      gTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAGTg                 <  1:429119/140‑1 (MQ=255)
                                                                                                                       ttACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAGTGc                >  1:190306/1‑140 (MQ=255)
                                                                                                                       ttACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAGTGc                <  2:689320/140‑1 (MQ=255)
                                                                                                                                cGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAGTGCTTTTTAGCa       >  2:138857/1‑140 (MQ=255)
                                                                                                                                  tgtTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAGTGCTTTTTAGCttt     >  1:612744/1‑140 (MQ=255)
                                                                                                                                     tcttcAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAGTGCTTTTTAGCATTTAg  <  1:483850/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
GATATCACGAAAACCACCAGCTGCAAGTTTATTAACTAAATGATGTTCTTGACCGTTCTTTTGACTAACATGAACTAAACTAGATGCAACGATATGAGGTAAATGACTTACGACGCTTGTTACGTAGTCGTGTTCTTCAGCAGTAGTTACAATAAATTTAGCAAGAGTAGGTGATAACAGTTCTTTTAACGTGTTTGCTGCTTGCTCATTTCTTGGCTCATTGTAGACTAAAATATAATAAGCGTTTTCAAATAAGTGCTTTTTAGCATTTAG  >  USA300TCH1516_ALE/1402004‑1402276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑