Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,458,972 T→C 5.5% D159G (GAT→GGT)  murG ← UDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,458,9720TC 5.5% 227.3 / 3.1 72D159G (GAT→GGT) murGUDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase
Reads supporting (aligned to +/- strand):  ref base T (25/43);  new base C (2/2);  total (27/45)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

ATGCTATTTAATTTTTTACTTCCTAAGCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATAAGAGTTGGTATATTTAATGAT  >  USA300TCH1516_ALE/1458834‑1459109
                                                                                                                                          |                                                                                                                                         
atGCTATTTAATTTTTTACTTCCTAAGCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATc                                                                                                                                          <  1:445578/140‑1 (MQ=255)
atGCTATTTAATTTTTTACCTCCGAAGCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATc                                                                                                                                          <  1:768289/140‑1 (MQ=255)
  gCTATTTAATTTTTTACTTCCTAAGCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAg                                                                                                                                        <  2:773119/140‑1 (MQ=255)
             ttttACTTCCTAAGCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGCTACGCAAGATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTa                                                                                                                             >  1:185656/1‑140 (MQ=255)
               ttACTTCCTAAGCTGCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAgg                                                                                                                           <  1:369369/140‑1 (MQ=255)
                    tCCTAAGCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAagt                                                                                                                      >  1:779779/1‑140 (MQ=255)
                    tCCTAAGCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAagt                                                                                                                      >  1:363725/1‑140 (MQ=255)
                       tAATCTTCTACCCATAACGTGTAAAATTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAagtagt                                                                                                                   <  2:389056/140‑1 (MQ=255)
                          gCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTa                                                                                                                <  1:331043/140‑1 (MQ=255)
                           cTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGtttcg                                                                                                               >  1:725256/1‑138 (MQ=255)
                           cTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAg                                                                                                               <  2:502592/140‑1 (MQ=255)
                           cTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTCTAGGTAGGTCGTTTAg                                                                                                               >  1:771114/1‑140 (MQ=255)
                            ttCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGc                                                                                                              >  1:555945/1‑140 (MQ=255)
                                   cATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTttctt                                                                                                       <  1:315067/140‑1 (MQ=255)
                                    gtAACGAGTAGAAATTTTTTATTTTCATTAAAGCCTGTTAATTGCTAACCATTATGTGCATTACCATTTTTTAAATCTTGTCGAATTGTTGCTCCAATAAAATCAGCTTTCTTGTTAGGTAAGTAGTTTAGCGTttcttc                                                                                                      <  2:15351/139‑1 (MQ=255)
                                    aTAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTTTTTTACATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTttcttc                                                                                                      <  2:357980/140‑1 (MQ=255)
                                     tAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCa                                                                                                     <  1:323683/140‑1 (MQ=255)
                                     tAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTGTTTGAATCGTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCa                                                                                                     <  1:588276/140‑1 (MQ=255)
                                        cGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAAACATTATGTGCATTACCATTTTTTAAATATTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAAt                                                                                                  >  1:534792/1‑140 (MQ=255)
                                          aGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAAtgt                                                                                                <  2:333918/140‑1 (MQ=255)
                                           gTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAAtgtt                                                                                               <  1:189901/140‑1 (MQ=255)
                                              aaaCTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGta                                                                                            <  1:535122/140‑1 (MQ=255)
                                                aCTTTTTTATTTTCATTAAACCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCACTCAAATCAGCTCTCTCTTTAGGTACGTGGTTTAGCGTTTCTTCAACACTTGtatc                                                                                          >  2:223840/1‑139 (MQ=255)
                                                 cTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGtatat                                                                                         <  1:585653/140‑1 (MQ=255)
                                                       tATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTc                                                                                   <  1:445535/140‑1 (MQ=255)
                                                         ttttCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCtt                                                                                 <  1:610677/140‑1 (MQ=255)
                                                           ttCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCATCTTTCTCTTTAGGTAAGTATTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTgg                                                                               <  1:443012/140‑1 (MQ=255)
                                                           ttCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTgg                                                                               >  2:345918/1‑140 (MQ=255)
                                                           ttCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTgg                                                                               <  1:543187/140‑1 (MQ=255)
                                                            tCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTCGTATATATTTTCTTGGc                                                                              >  1:131276/1‑140 (MQ=255)
                                                                  aaGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAAttt                                                                        <  2:95326/140‑1 (MQ=255)
                                                                          tAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAACCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTa                                                                >  2:418442/1‑140 (MQ=255)
                                                                          tAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAACCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTa                                                                >  2:418441/1‑140 (MQ=255)
                                                                            aTTGATAACCATTATGTGCATTACGATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATc                                                              <  2:783225/140‑1 (MQ=255)
                                                                            aTTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCACTAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTCGGCAAATTTAAGTGCTATc                                                              >  2:741131/1‑140 (MQ=255)
                                                                            aTTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATc                                                              <  2:759417/140‑1 (MQ=255)
                                                                               gATAACCATTATGTGCATTACCATTTTTTATATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTa                                                           <  2:662567/140‑1 (MQ=255)
                                                                                     cATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCt                                                     >  2:701998/1‑140 (MQ=255)
                                                                                     cATTATGTGCATTACCATTTTTTAAAGCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTTGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCt                                                     <  1:301953/140‑1 (MQ=255)
                                                                                       ttATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTaa                                                   >  1:538881/1‑140 (MQ=255)
                                                                                       ttATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTaa                                                   >  1:124116/1‑140 (MQ=255)
                                                                                         aTGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATc                                                 >  1:516686/1‑140 (MQ=255)
                                                                                          tgtgCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTTATcc                                                >  2:294247/1‑140 (MQ=255)
                                                                                             gCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTGGTTTGGCCAATCGTCCAATGTTGTTTGTGTTTGCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTgg                                             <  1:187787/140‑1 (MQ=255)
                                                                                             gCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTgg                                             <  1:417362/140‑1 (MQ=255)
                                                                                             gCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTgg                                             <  1:726192/140‑1 (MQ=255)
                                                                                              cATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGgt                                            <  2:497342/140‑1 (MQ=255)
                                                                                              cATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGgt                                            <  2:198979/140‑1 (MQ=255)
                                                                                                 tACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTGCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGtt                                         >  1:697880/1‑140 (MQ=255)
                                                                                                 tACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGATTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTCATTGGCAAATGTAAGTGCTATCTTATTCGCTGATCCTgttgtt                                         >  1:275266/1‑140 (MQ=255)
                                                                                                    cATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAACATCAGATTTCTCTTTAGGTAAGTAGTTTAGAGTTTATTCATATGTTGTATATATTTTCTTGGCAAATATAAGTGTTGTCTTTATCGCTAATCCTGGTGTTAAg                                      <  2:534792/140‑1 (MQ=255)
                                                                                                    cATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAg                                      <  1:354490/140‑1 (MQ=255)
                                                                                                    cATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAg                                      <  1:25511/140‑1 (MQ=255)
                                                                                                    cATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAg                                      >  2:457610/1‑140 (MQ=255)
                                                                                                     aTTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGATACCATAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGt                                     <  2:363725/140‑1 (MQ=255)
                                                                                                      ttttttAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTc                                    <  1:749696/140‑1 (MQ=255)
                                                                                                      ttttttAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGCATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTc                                    >  2:664799/1‑140 (MQ=255)
                                                                                                        ttttAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAg                                  <  2:473382/140‑1 (MQ=255)
                                                                                                         tttAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGa                                 <  1:382564/140‑1 (MQ=255)
                                                                                                         tttAAATCTTCTCGAATTGTTGCTCCAATAAAACCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGa                                 <  1:418442/140‑1 (MQ=255)
                                                                                                         tttAAATCTTCTCGAATTGTTGCTCCAATAAAACCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGa                                 <  1:418441/140‑1 (MQ=255)
                                                                                                           tAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGAtt                               <  1:161207/140‑1 (MQ=255)
                                                                                                           tAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGAtt                               <  1:82239/140‑1 (MQ=255)
                                                                                                               tcttctCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTTTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATg                           >  2:296017/1‑140 (MQ=255)
                                                                                                                   ctcGAATTGTTGCTGCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATa                       <  2:277559/140‑1 (MQ=255)
                                                                                                                   ctcGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATa                       <  1:701998/140‑1 (MQ=255)
                                                                                                                   ctcGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATa                       <  1:60689/140‑1 (MQ=255)
                                                                                                                         ttgttgCTCCAATAAAATCAGCTTTCTCTTTATGTAAGTAGTTTAGCGTTTCTTCAGATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATAAGAGtt                 <  2:632245/140‑1 (MQ=255)
                                                                                                                            ttgCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATAAGAGTTGGt              >  2:478677/1‑140 (MQ=255)
                                                                                                                            ttgCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATAAGAGTTGGt              >  1:334003/1‑140 (MQ=255)
                                                                                                                                  cAATAAAATCAGCTTTCTCTTTAGGTACGTAGTTTAGCGATTCTTCAAATGTTGTATATATTTTATTGGCAAATTTAAGTGCTGTCTTATTCTCTAATCTTGGTGTTAAGACCGTTTCAAGACTAAGATATGGTATCttt        >  2:289764/1‑140 (MQ=255)
                                                                                                                                        aaTCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATAAGAGTTGGTATATTTAATGAt  >  2:613271/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
ATGCTATTTAATTTTTTACTTCCTAAGCTTCCACCCATAACGAGTAAAACTTTTTTATTTTCATTAAAGCCTGTTAATTGATAACCATTATGTGCATTACCATTTTTTAAATCTTCTCGAATTGTTGCTCCAATAAAATCAGCTTTCTCTTTAGGTAAGTAGTTTAGCGTTTCTTCAAATGTTGTATATATTTTCTTGGCAAATTTAAGTGCTATCTTATTCGCTAATCCTGGTGTTAAGTCAGATTCATGAATAAGAGTTGGTATATTTAATGAT  >  USA300TCH1516_ALE/1458834‑1459109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: