Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 452,493 C→T 14.6% S116L (TCA→TTA)  USA300HOU_RS02140 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE452,4930CT14.6% 166.9 / 16.7 75S116L (TCA→TTA) USA300HOU_RS02140hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (27/35);  new base T (8/3);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGTTTGGAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAATCTCGTTAAA  >  USA300TCH1516_ALE/452357‑452631
                                                                                                                                        |                                                                                                                                          
tatGTTTGGAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGATAATGGAAGACAATATTGAAt                                                                                                                                         >  2:472508/1‑140 (MQ=255)
 atGTTTGGAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAAtt                                                                                                                                        <  1:515899/140‑1 (MQ=255)
 aggTTGGGAATGATTGAAAATAGTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCTAGGTGAGCATGATAAGCAAGAGGCATTTTTAGTCATCGAGGAGGTAACTGTTAATGGAAGACAATATTTAAtt                                                                                                                                        <  2:716024/138‑1 (MQ=255)
    tttGGAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGATACTGTTAATGGAAGACAATATTCAATtggt                                                                                                                                     <  1:154388/140‑1 (MQ=255)
    tttGGAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATtggt                                                                                                                                     <  2:40190/140‑1 (MQ=255)
    tttGGAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATtggt                                                                                                                                     <  1:380207/140‑1 (MQ=255)
    tgtGGAACAATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATtggt                                                                                                                                     <  1:707703/138‑1 (MQ=255)
       ggAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTggtggt                                                                                                                                  >  2:37441/1‑140 (MQ=255)
        gAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGtt                                                                                                                                 <  1:37968/140‑1 (MQ=255)
          aGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATAATTAATTGGAGGTTta                                                                                                                               >  2:656476/1‑140 (MQ=255)
           ggATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACTCTCAAGTTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTGtag                                                                                                                              >  2:203015/1‑139 (MQ=255)
           atgtCGAAAAGACTATTTTCTTGGCGTGCTTAGTAATGATATTGAAACATACCCTCAAGGTGAGCATGATAAGCAAGCTGCATTTTTAGTCATCGAGGAGGACACTGTTAATGGAAGACAATATTCAATTGGTGGTTtaa                                                                                                                              <  2:187611/137‑1 (MQ=255)
            gATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTtaag                                                                                                                             >  1:759501/1‑140 (MQ=255)
            gATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATAGGTGGCTcaag                                                                                                                             >  2:546327/1‑140 (MQ=255)
               tgAAAAGATTATTTTGTTGGCGTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTtaagtaa                                                                                                                          <  2:457918/139‑1 (MQ=255)
               cGAAAAGTGTATGTTGTTGTCTAGCTTGGTAAAGATATTCAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCAGTTGTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTtaagtaa                                                                                                                          <  2:462066/140‑1 (MQ=255)
               cGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTtaagtaa                                                                                                                          <  1:702694/140‑1 (MQ=255)
                gAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGCAGACAATATTCAATTGGTGGCTtaagtaag                                                                                                                         >  2:151920/1‑140 (MQ=255)
                 aaaaGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGTGGACAATATTCAATTGGTGGTTTAAGTAAGa                                                                                                                        >  1:146967/1‑140 (MQ=255)
                 aaaaGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGa                                                                                                                        >  1:416755/1‑140 (MQ=255)
                   aaGATTATGTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACa                                                                                                                      <  1:230603/140‑1 (MQ=255)
                         attTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGt                                                                                                                <  1:251204/140‑1 (MQ=255)
                          ttttGTTGGCTTGCTTCGCAAACATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGACGCATTTTTAGTCATCGAGGAGGAAACTGGTTAAGGCGTACAATATTCAATTGCTGGTTTAATTAAGACAAATAGTa                                                                                                               <  1:722987/140‑1 (MQ=255)
                               tcggCTGGCGTGGACAAGATATCGAAACAGACCCACACGGGGAGCATGCTAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATCCCATTGGTGGTTTAAGTAAGACAAATAGTAAAGaa                                                                                                          <  1:163642/138‑1 (MQ=255)
                                  gcttgcttGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAAttt                                                                                                       <  1:431757/140‑1 (MQ=255)
                                   cttgcttgGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTa                                                                                                      >  1:740976/1‑140 (MQ=255)
                                      gcttgGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATAATAAGCAAGAAGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTa                                                                                                   <  1:610812/140‑1 (MQ=255)
                                             aaaGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGt                                                                                            >  2:149758/1‑140 (MQ=255)
                                              aaGATATTGAAAAGTACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTc                                                                                           <  2:546862/140‑1 (MQ=255)
                                              aaGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTc                                                                                           <  1:566856/140‑1 (MQ=255)
                                              aaGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTc                                                                                           <  2:176794/140‑1 (MQ=255)
                                              aaGATATTGAAAAAGACCGTGAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTc                                                                                           <  1:31883/140‑1 (MQ=255)
                                              aaGATAGTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTc                                                                                           <  2:622991/140‑1 (MQ=255)
                                                gATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGa                                                                                         >  2:731547/1‑140 (MQ=255)
                                                 atatTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGAt                                                                                        >  1:783315/1‑140 (MQ=255)
                                                   atTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGt                                                                                      <  1:747991/140‑1 (MQ=255)
                                                   atTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTACAGAAGTCGATGt                                                                                      >  1:626633/1‑140 (MQ=255)
                                                        aaaaTACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAAACGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAg                                                                                 >  1:507219/1‑140 (MQ=255)
                                                         aaaTACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGCATAGATTAAAGAAGTCGATGTTAAAGt                                                                                >  2:3308/1‑140 (MQ=255)
                                                         aaaTACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGt                                                                                >  2:62260/1‑140 (MQ=255)
                                                              cccTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACaa                                                                           >  1:291851/1‑140 (MQ=255)
                                                               ccTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAg                                                                          >  2:259524/1‑140 (MQ=255)
                                                               ccTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAg                                                                          >  2:151160/1‑140 (MQ=255)
                                                                cTCAAGGTGAGTATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGa                                                                         <  2:780183/140‑1 (MQ=255)
                                                                cTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATGCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTCAGTAAAGAAGTCGATGTTAAAGTAACAAGa                                                                         >  1:407093/1‑140 (MQ=255)
                                                                atctatgTGAGCATGATAATCTAGATTCATTTTTAGTTATCGAGGATGAAACTGTTAATGGAATACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGa                                                                         <  2:645618/134‑1 (MQ=255)
                                                                 tCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGaa                                                                        >  2:278818/1‑140 (MQ=255)
                                                                  cAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTCAAGAAGTCGATGTTAAAGTAACAAGaaa                                                                       >  1:165777/1‑140 (MQ=255)
                                                                  cAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGaaa                                                                       >  1:159847/1‑140 (MQ=255)
                                                                         aGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATtgat                                                                <  2:715933/140‑1 (MQ=255)
                                                                           cgtGATAAGCGAGATGGATTTCTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATtgatga                                                              <  2:450033/138‑1 (MQ=255)
                                                                                         tGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGt                                                <  1:601215/140‑1 (MQ=255)
                                                                                          gCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTc                                               >  2:234785/1‑140 (MQ=255)
                                                                                          gCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATGTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAGAGtg                                               >  2:635384/1‑139 (MQ=255)
                                                                                           cATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCt                                              >  2:587908/1‑140 (MQ=255)
                                                                                           cATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATAGGAAAAgcat                                              >  2:617117/1‑137 (MQ=255)
                                                                                             tttttAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTaa                                            <  1:37441/140‑1 (MQ=255)
                                                                                               tttAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAg                                          <  1:292412/140‑1 (MQ=255)
                                                                                                      aTCGAGGAGGAAACTGTTAATGGAAGACAACATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTaa                                   <  2:110021/140‑1 (MQ=255)
                                                                                                          aggaggAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAAttt                               >  2:697844/1‑140 (MQ=255)
                                                                                                            gaggaAACTGTTAATGGAAGACAATATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTaa                             <  2:603025/140‑1 (MQ=255)
                                                                                                            gaggaAACTGTAAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGAAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTaa                             <  2:199863/140‑1 (MQ=255)
                                                                                                            gaggaAACGTTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGAAAAATAGTAAAGAATGTAGAAAAGGAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTaa                             <  2:199792/140‑1 (MQ=255)
                                                                                                             gggaAACTGTTGATGGAGGAGAAGATTGAATTGGGGGTTTGAGGAAGATAAAGAGTAAAGAATGTAGTAAAGAAGGGGATGTTAAAGTAATAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTaaa                            <  2:225342/139‑1 (MQ=255)
                                                                                                               gaAACTGTTAATGGAAGACAATATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAAt                          >  2:611269/1‑140 (MQ=255)
                                                                                                               gaAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAAt                          >  2:52817/1‑140 (MQ=255)
                                                                                                               gaAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAAt                          >  2:142055/1‑140 (MQ=255)
                                                                                                                             aaGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGaaa            >  2:754651/1‑140 (MQ=255)
                                                                                                                               gACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAAtc          <  1:133782/140‑1 (MQ=255)
                                                                                                                                  aaTATTTAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAATCTCg       >  1:706389/1‑140 (MQ=255)
                                                                                                                                    tatTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAATCTCGtt     >  1:108352/1‑140 (MQ=255)
                                                                                                                                    tatTCAATTGGTGGTTTAAGTAAGACAAAAAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAATCTCGtt     <  1:539430/140‑1 (MQ=255)
                                                                                                                                    tatTCAATTGGCGGTTTAAGCAAGACAAATAGTAAAGAATTGAGTAAAGAAGTCGATGATAAAGTAACAAGAAAAATTGATGAATCATCGGAGAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAATCTCGtt     >  2:436116/1‑140 (MQ=255)
                                                                                                                                     atTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAATCTCGTTa    <  2:108352/140‑1 (MQ=255)
                                                                                                                                       tCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAATCTCGTTaaa  <  1:611/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TATGTTTGGAAGGATCGAAAAGATTATTTTGTTGGCTTGCTTGGTAAAGATATTGAAAAATACCCTCAAGGTGAGCATGATAAGCAAGATGCATTTTTAGTCATCGAGGAGGAAACTGTTAATGGAAGACAATATTCAATTGGTGGTTTAAGTAAGACAAATAGTAAAGAATTTAGTAAAGAAGTCGATGTTAAAGTAACAAGAAAAATTGATGAATCATCGGAAAAGTCTAAAGATAGTAAATTTAAAATTACTAAAGAAGAAATCTCGTTAAA  >  USA300TCH1516_ALE/452357‑452631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑